Mitochondrial Transplantation Promotes Remyelination and Long-Term Locomotion Recovery following Cerebral Ischemia
Cerebral ischemia usually leads to axonal degeneration and demyelination in the adjacent white matter. Promoting remyelination still remains a challenging issue in the field. Considering that ischemia deprives energy supply to neural cells and high metabolic activities are required by oligodendrocyt...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2022/1346343 |
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| author | Tao Chen Yuanyuan Zhu Jia Jia Han Meng Chao Xu Panpan Xian Zijie Li Zhengang Tang Yin Wu Yan Liu |
| author_facet | Tao Chen Yuanyuan Zhu Jia Jia Han Meng Chao Xu Panpan Xian Zijie Li Zhengang Tang Yin Wu Yan Liu |
| author_sort | Tao Chen |
| collection | DOAJ |
| description | Cerebral ischemia usually leads to axonal degeneration and demyelination in the adjacent white matter. Promoting remyelination still remains a challenging issue in the field. Considering that ischemia deprives energy supply to neural cells and high metabolic activities are required by oligodendrocyte progenitor cells (OPCs) for myelin formation, we assessed the effects of transplanting exogenous healthy mitochondria on the degenerating process of oligodendrocytes following focal cerebral ischemia in the present study. Our results showed that exogenous mitochondria could efficiently restore the overall mitochondrial function and be effectively internalized by OPCs in the ischemic cortex. In comparison with control cortex, there were significantly less apoptotic and more proliferative OPCs in mitochondria-treated cortex. More importantly, higher levels of myelin basic protein (MBP) and more morphologically normal myelin-wrapped axons were observed in mitochondria-treated cortex at 21 days postinjury, as revealed by light and electron microscope. Behavior assay showed better locomotion recovery in mitochondria-treated mice. Further analysis showed that olig2 and lipid synthesis signaling were significantly increased in mitochondria-treated cortex. In together, our data illustrated an antidegenerating and myelination-promoting effect of exogenous mitochondria, indicating mitochondria transplantation as a potentially valuable treatment for ischemic stroke. |
| format | Article |
| id | doaj-art-8582876f26ad460fb416fd7e6d65bc41 |
| institution | Kabale University |
| issn | 1466-1861 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-8582876f26ad460fb416fd7e6d65bc412025-08-20T03:37:47ZengWileyMediators of Inflammation1466-18612022-01-01202210.1155/2022/1346343Mitochondrial Transplantation Promotes Remyelination and Long-Term Locomotion Recovery following Cerebral IschemiaTao Chen0Yuanyuan Zhu1Jia Jia2Han Meng3Chao Xu4Panpan Xian5Zijie Li6Zhengang Tang7Yin Wu8Yan Liu9Branch of Cerebral Vascular DiseasesDepartment of Neurobiology and Institute of NeurosciencesDepartment of GastroenterologyDepartment of Neurobiology and Institute of NeurosciencesDepartment of Neurobiology and Institute of NeurosciencesDepartment of Neurobiology and Institute of NeurosciencesDepartment of Neurobiology and Institute of NeurosciencesInstitute of NeurologyDepartment of PharmacyBranch of Cerebral Vascular DiseasesCerebral ischemia usually leads to axonal degeneration and demyelination in the adjacent white matter. Promoting remyelination still remains a challenging issue in the field. Considering that ischemia deprives energy supply to neural cells and high metabolic activities are required by oligodendrocyte progenitor cells (OPCs) for myelin formation, we assessed the effects of transplanting exogenous healthy mitochondria on the degenerating process of oligodendrocytes following focal cerebral ischemia in the present study. Our results showed that exogenous mitochondria could efficiently restore the overall mitochondrial function and be effectively internalized by OPCs in the ischemic cortex. In comparison with control cortex, there were significantly less apoptotic and more proliferative OPCs in mitochondria-treated cortex. More importantly, higher levels of myelin basic protein (MBP) and more morphologically normal myelin-wrapped axons were observed in mitochondria-treated cortex at 21 days postinjury, as revealed by light and electron microscope. Behavior assay showed better locomotion recovery in mitochondria-treated mice. Further analysis showed that olig2 and lipid synthesis signaling were significantly increased in mitochondria-treated cortex. In together, our data illustrated an antidegenerating and myelination-promoting effect of exogenous mitochondria, indicating mitochondria transplantation as a potentially valuable treatment for ischemic stroke.http://dx.doi.org/10.1155/2022/1346343 |
| spellingShingle | Tao Chen Yuanyuan Zhu Jia Jia Han Meng Chao Xu Panpan Xian Zijie Li Zhengang Tang Yin Wu Yan Liu Mitochondrial Transplantation Promotes Remyelination and Long-Term Locomotion Recovery following Cerebral Ischemia Mediators of Inflammation |
| title | Mitochondrial Transplantation Promotes Remyelination and Long-Term Locomotion Recovery following Cerebral Ischemia |
| title_full | Mitochondrial Transplantation Promotes Remyelination and Long-Term Locomotion Recovery following Cerebral Ischemia |
| title_fullStr | Mitochondrial Transplantation Promotes Remyelination and Long-Term Locomotion Recovery following Cerebral Ischemia |
| title_full_unstemmed | Mitochondrial Transplantation Promotes Remyelination and Long-Term Locomotion Recovery following Cerebral Ischemia |
| title_short | Mitochondrial Transplantation Promotes Remyelination and Long-Term Locomotion Recovery following Cerebral Ischemia |
| title_sort | mitochondrial transplantation promotes remyelination and long term locomotion recovery following cerebral ischemia |
| url | http://dx.doi.org/10.1155/2022/1346343 |
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