Transcriptomics analysis identifies critical genes involved in the infection of crandell-reese feline kidney cell lines by feline panleukopenia virus
Feline panleukopenia virus (FPV) is an extremely contagious pathogen that induces severe vomiting, diarrhea, and dehydration in cats, often resulting in high mortality rates and substantial economic losses. Host responses are crucial for viral entry, replication, assembly, and disease progression, d...
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Kafkas University, Faculty of Veterinary Medicine
2025-05-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
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| Online Access: | https://vetdergikafkas.org/pdf.php?id=3194 |
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| author | Shengnan SONG Pan CHEN Chuangfu CHEN Jia GUO |
| author_facet | Shengnan SONG Pan CHEN Chuangfu CHEN Jia GUO |
| author_sort | Shengnan SONG |
| collection | DOAJ |
| description | Feline panleukopenia virus (FPV) is an extremely contagious pathogen that induces
severe vomiting, diarrhea, and dehydration in cats, often resulting in high mortality rates
and substantial economic losses. Host responses are crucial for viral entry, replication,
assembly, and disease progression, despite so much is not known, especially regarding
the interactions between hosts and viruses. Herein, we employed the FPV strain FPVXJ-
04, which was previously isolated and characterized by our team in Xinjiang, China.
To perform a comparative transcriptomic analysis of the gene expression profile in
Crandell-Reese feline kidney (CRFK) cells following infection with the FPV-XJ-04
strain. Following infection of CRFK cells with the FPV-XJ-04 strain, the differentially
expressed genes in CRFK cells were predicted to be involved in several key signaling
pathways, including the TNF signaling pathway, IL-17 signaling pathway, cytokinecytokine
receptor interaction, Toll-like receptor signaling pathway, MAPK signaling
pathway, and RIG-I-like receptor signaling pathway. In addition, the immune response
was significantly enriched in the Gene Ontology (GO) and Kyoto Encyclopedia of Genes
and Genomes (KEGG) pathways. Furthermore, we highlight 11 key genes in the TNF
signaling pathway that are associated with host defense response to the invasion of
FPV-XJ-04 strain. This research provides valuable insights into the gene transcription
processes within immune cells, elucidating the pathways critical to the early stages of
infection pathogenesis. |
| format | Article |
| id | doaj-art-8570ff05929a4c38ad66129ad29cd233 |
| institution | Kabale University |
| issn | 1309-2251 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj-art-8570ff05929a4c38ad66129ad29cd2332025-08-20T03:26:34ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512025-05-0131341141710.9775/kvfd.2025.336983194Transcriptomics analysis identifies critical genes involved in the infection of crandell-reese feline kidney cell lines by feline panleukopenia virusShengnan SONG0Pan CHEN1Chuangfu CHEN2Jia GUO3State International Joint Research Center for Animal Health Breeding, College of Animal Science and Technology, Shihezi University, Shihezi 832003, CHINAState International Joint Research Center for Animal Health Breeding, College of Animal Science and Technology, Shihezi University, Shihezi 832003, CHINAState International Joint Research Center for Animal Health Breeding, College of Animal Science and Technology, Shihezi University, Shihezi 832003, CHINAState International Joint Research Center for Animal Health Breeding, College of Animal Science and Technology, Shihezi University, Shihezi 832003, CHINAFeline panleukopenia virus (FPV) is an extremely contagious pathogen that induces severe vomiting, diarrhea, and dehydration in cats, often resulting in high mortality rates and substantial economic losses. Host responses are crucial for viral entry, replication, assembly, and disease progression, despite so much is not known, especially regarding the interactions between hosts and viruses. Herein, we employed the FPV strain FPVXJ- 04, which was previously isolated and characterized by our team in Xinjiang, China. To perform a comparative transcriptomic analysis of the gene expression profile in Crandell-Reese feline kidney (CRFK) cells following infection with the FPV-XJ-04 strain. Following infection of CRFK cells with the FPV-XJ-04 strain, the differentially expressed genes in CRFK cells were predicted to be involved in several key signaling pathways, including the TNF signaling pathway, IL-17 signaling pathway, cytokinecytokine receptor interaction, Toll-like receptor signaling pathway, MAPK signaling pathway, and RIG-I-like receptor signaling pathway. In addition, the immune response was significantly enriched in the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Furthermore, we highlight 11 key genes in the TNF signaling pathway that are associated with host defense response to the invasion of FPV-XJ-04 strain. This research provides valuable insights into the gene transcription processes within immune cells, elucidating the pathways critical to the early stages of infection pathogenesis.https://vetdergikafkas.org/pdf.php?id=3194feline panleukopenia virushost-virus interactionsimmune responsecomparative transcriptomic analysistnf signaling pathway |
| spellingShingle | Shengnan SONG Pan CHEN Chuangfu CHEN Jia GUO Transcriptomics analysis identifies critical genes involved in the infection of crandell-reese feline kidney cell lines by feline panleukopenia virus Kafkas Universitesi Veteriner Fakültesi Dergisi feline panleukopenia virus host-virus interactions immune response comparative transcriptomic analysis tnf signaling pathway |
| title | Transcriptomics analysis identifies critical genes involved in the infection of crandell-reese feline kidney cell lines by feline panleukopenia virus |
| title_full | Transcriptomics analysis identifies critical genes involved in the infection of crandell-reese feline kidney cell lines by feline panleukopenia virus |
| title_fullStr | Transcriptomics analysis identifies critical genes involved in the infection of crandell-reese feline kidney cell lines by feline panleukopenia virus |
| title_full_unstemmed | Transcriptomics analysis identifies critical genes involved in the infection of crandell-reese feline kidney cell lines by feline panleukopenia virus |
| title_short | Transcriptomics analysis identifies critical genes involved in the infection of crandell-reese feline kidney cell lines by feline panleukopenia virus |
| title_sort | transcriptomics analysis identifies critical genes involved in the infection of crandell reese feline kidney cell lines by feline panleukopenia virus |
| topic | feline panleukopenia virus host-virus interactions immune response comparative transcriptomic analysis tnf signaling pathway |
| url | https://vetdergikafkas.org/pdf.php?id=3194 |
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