Identification of the gene coding for seed cotyledon albumin SCA in the pea (Pisum L.) genome
Albumins SCA and SAA are short, highly hydrophilic proteins accumulated in large quantities in the cotyledons and seed axes, respectively, of a dry pea (Pisum sativum L.) seed. SCA was earlier shown to have two allelic variants differing in mobility in polyacrylamide gel electrophoresis in acid medi...
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2022-07-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/3385 |
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| author | A. V. Mglinets V. S. Bogdanova O. E. Kosterin |
| author_facet | A. V. Mglinets V. S. Bogdanova O. E. Kosterin |
| author_sort | A. V. Mglinets |
| collection | DOAJ |
| description | Albumins SCA and SAA are short, highly hydrophilic proteins accumulated in large quantities in the cotyledons and seed axes, respectively, of a dry pea (Pisum sativum L.) seed. SCA was earlier shown to have two allelic variants differing in mobility in polyacrylamide gel electrophoresis in acid medium. Using them, the corresponding gene SCA was mapped on Linkage Group V. This protein was used as a useful genetic and phylogeographical marker, which still required electrophoretic analysis of the protein while the DNA sequence of the corresponding SCA gene remained unknown. Based on the length, the positive charge under acidic conditions and the number of lysine residues of SCA and SAA albumins, estimated earlier electrophoretically, the data available in public databases were searched for candidates for the SCA gene among coding sequences residing in the region of the pea genome which, taking into account the synteny of the pea and Medicago truncatula genomes, corresponds to the map position of SCA. Then we sequenced them in a number of pea accessions. Concordance of the earlier electrophoretic data and sequence variation indicated the sequence Psat0s797g0160 of the reference pea genome to be the SCA gene. The sequence Psat0s797g0240 could encode a minor related albumin SA-a2, while a candidate gene for albumin SAA is still missing (as well as electrophoretic variation of both latter albumins). DNA amplification using original primers SCA1_3f and SCA1_3r from genomic DNA and restriction by endonuclease HindII made it possible to distinguish the SCA alleles coding for protein products with different charges without sequencing the gene. Thus, the gene encoding the highly hydrophilic albumin SCA accumulated in pea seeds, the alleles of which are useful for classification of pea wild relatives, has now been identified in the pea genome and a convenient CAPS marker has been developed on its basis. |
| format | Article |
| id | doaj-art-8563c888422546aab4b545c4ff613fe2 |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2022-07-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-8563c888422546aab4b545c4ff613fe22025-02-01T09:58:11ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592022-07-0126435936410.18699/VJGB-22-431268Identification of the gene coding for seed cotyledon albumin SCA in the pea (Pisum L.) genomeA. V. Mglinets0V. S. Bogdanova1O. E. Kosterin2Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of SciencesInstitute of Cytology and Genetics of the Siberian Branch of the Russian Academy of SciencesInstitute of Cytology and Genetics of the Siberian Branch of the Russian Academy of SciencesAlbumins SCA and SAA are short, highly hydrophilic proteins accumulated in large quantities in the cotyledons and seed axes, respectively, of a dry pea (Pisum sativum L.) seed. SCA was earlier shown to have two allelic variants differing in mobility in polyacrylamide gel electrophoresis in acid medium. Using them, the corresponding gene SCA was mapped on Linkage Group V. This protein was used as a useful genetic and phylogeographical marker, which still required electrophoretic analysis of the protein while the DNA sequence of the corresponding SCA gene remained unknown. Based on the length, the positive charge under acidic conditions and the number of lysine residues of SCA and SAA albumins, estimated earlier electrophoretically, the data available in public databases were searched for candidates for the SCA gene among coding sequences residing in the region of the pea genome which, taking into account the synteny of the pea and Medicago truncatula genomes, corresponds to the map position of SCA. Then we sequenced them in a number of pea accessions. Concordance of the earlier electrophoretic data and sequence variation indicated the sequence Psat0s797g0160 of the reference pea genome to be the SCA gene. The sequence Psat0s797g0240 could encode a minor related albumin SA-a2, while a candidate gene for albumin SAA is still missing (as well as electrophoretic variation of both latter albumins). DNA amplification using original primers SCA1_3f and SCA1_3r from genomic DNA and restriction by endonuclease HindII made it possible to distinguish the SCA alleles coding for protein products with different charges without sequencing the gene. Thus, the gene encoding the highly hydrophilic albumin SCA accumulated in pea seeds, the alleles of which are useful for classification of pea wild relatives, has now been identified in the pea genome and a convenient CAPS marker has been developed on its basis.https://vavilov.elpub.ru/jour/article/view/3385late embryogenesis proteinsseed cotyledon albuminpeaspisum sativum l.caps marker |
| spellingShingle | A. V. Mglinets V. S. Bogdanova O. E. Kosterin Identification of the gene coding for seed cotyledon albumin SCA in the pea (Pisum L.) genome Вавиловский журнал генетики и селекции late embryogenesis proteins seed cotyledon albumin peas pisum sativum l. caps marker |
| title | Identification of the gene coding for seed cotyledon albumin SCA in the pea (Pisum L.) genome |
| title_full | Identification of the gene coding for seed cotyledon albumin SCA in the pea (Pisum L.) genome |
| title_fullStr | Identification of the gene coding for seed cotyledon albumin SCA in the pea (Pisum L.) genome |
| title_full_unstemmed | Identification of the gene coding for seed cotyledon albumin SCA in the pea (Pisum L.) genome |
| title_short | Identification of the gene coding for seed cotyledon albumin SCA in the pea (Pisum L.) genome |
| title_sort | identification of the gene coding for seed cotyledon albumin sca in the pea pisum l genome |
| topic | late embryogenesis proteins seed cotyledon albumin peas pisum sativum l. caps marker |
| url | https://vavilov.elpub.ru/jour/article/view/3385 |
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