Spontaneous and Induced Platelet Aggregation during Pregnancy and Labor
Objective: to evaluate changes in characteristics of spontaneous platelet (Pt) aggregation in patients with obstetric complications associated with hereditary thrombophilia.Materials and methods. Blood samples were taken from 52 recently confined women on the first day after labor; at that, ethic re...
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Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia
2017-01-01
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| Series: | Общая реаниматология |
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| Online Access: | https://www.reanimatology.com/rmt/article/view/1550 |
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| author | T. P. Bondar A. Yu. Muratova E. A. Melchenko |
| author_facet | T. P. Bondar A. Yu. Muratova E. A. Melchenko |
| author_sort | T. P. Bondar |
| collection | DOAJ |
| description | Objective: to evaluate changes in characteristics of spontaneous platelet (Pt) aggregation in patients with obstetric complications associated with hereditary thrombophilia.Materials and methods. Blood samples were taken from 52 recently confined women on the first day after labor; at that, ethic regulations for the preanalytical phase were followed. Determination of PlA1/ PlA2 polymorphism enotype was performed by means of amplificationrestriction analysis. Geometrical characteristics of patients' peripheral blood Pt aggregation were studied by means of AFM Integra Prima. The degree of confidence of the parameters under test was determined using the ttest, and the significance level was considered valid at P<0.05.Results. A statistical analysis of the findings demonstrated that the length of Pt aggregates in healthy pregnant women was significantly higher than that in healthy nonpregnant women at all study phases. Patients with the P1A1/P1A2 polymorphism in the GP IIb/IIIa Pt receptor gene demonstrated increased widthm height, and density of Pt aggregates. The changes were most significant during the incubation phase lasting for 15 and 30 minutes. The study of geometric parameters of different exposures demonstrated the following: the longer the incubation period, the greater the difference between geometric parameters of the aggregates (e.g. height, length, and width). Conclusion. The analysis of obtained data demonstrated that the presence of P1A1/P1A2 polymorphism in GP IIb/IIIa Pt gene receptor contributes to the decrease in the platelet response threshold and enhances the spontaneous Pt aggregation. The imaging of aggregates provides strong evidence for the accelerated growth of the aggregates in thrombotic complications of pregnancy. |
| format | Article |
| id | doaj-art-85523e2891484d41bea92ed0eb36f347 |
| institution | DOAJ |
| issn | 1813-9779 2411-7110 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia |
| record_format | Article |
| series | Общая реаниматология |
| spelling | doaj-art-85523e2891484d41bea92ed0eb36f3472025-08-20T02:59:34ZengFederal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, RussiaОбщая реаниматология1813-97792411-71102017-01-01125162210.15360/1813-9779-2016-5-16-221524Spontaneous and Induced Platelet Aggregation during Pregnancy and LaborT. P. Bondar0A. Yu. Muratova1E. A. Melchenko2North Caucasus Federal UniversityNorth Caucasus Federal UniversityNorth Caucasus Federal UniversityObjective: to evaluate changes in characteristics of spontaneous platelet (Pt) aggregation in patients with obstetric complications associated with hereditary thrombophilia.Materials and methods. Blood samples were taken from 52 recently confined women on the first day after labor; at that, ethic regulations for the preanalytical phase were followed. Determination of PlA1/ PlA2 polymorphism enotype was performed by means of amplificationrestriction analysis. Geometrical characteristics of patients' peripheral blood Pt aggregation were studied by means of AFM Integra Prima. The degree of confidence of the parameters under test was determined using the ttest, and the significance level was considered valid at P<0.05.Results. A statistical analysis of the findings demonstrated that the length of Pt aggregates in healthy pregnant women was significantly higher than that in healthy nonpregnant women at all study phases. Patients with the P1A1/P1A2 polymorphism in the GP IIb/IIIa Pt receptor gene demonstrated increased widthm height, and density of Pt aggregates. The changes were most significant during the incubation phase lasting for 15 and 30 minutes. The study of geometric parameters of different exposures demonstrated the following: the longer the incubation period, the greater the difference between geometric parameters of the aggregates (e.g. height, length, and width). Conclusion. The analysis of obtained data demonstrated that the presence of P1A1/P1A2 polymorphism in GP IIb/IIIa Pt gene receptor contributes to the decrease in the platelet response threshold and enhances the spontaneous Pt aggregation. The imaging of aggregates provides strong evidence for the accelerated growth of the aggregates in thrombotic complications of pregnancy.https://www.reanimatology.com/rmt/article/view/1550platelet aggregation activitypregnant womengene polymorphismscanning probe microscopythrombophilia |
| spellingShingle | T. P. Bondar A. Yu. Muratova E. A. Melchenko Spontaneous and Induced Platelet Aggregation during Pregnancy and Labor Общая реаниматология platelet aggregation activity pregnant women gene polymorphism scanning probe microscopy thrombophilia |
| title | Spontaneous and Induced Platelet Aggregation during Pregnancy and Labor |
| title_full | Spontaneous and Induced Platelet Aggregation during Pregnancy and Labor |
| title_fullStr | Spontaneous and Induced Platelet Aggregation during Pregnancy and Labor |
| title_full_unstemmed | Spontaneous and Induced Platelet Aggregation during Pregnancy and Labor |
| title_short | Spontaneous and Induced Platelet Aggregation during Pregnancy and Labor |
| title_sort | spontaneous and induced platelet aggregation during pregnancy and labor |
| topic | platelet aggregation activity pregnant women gene polymorphism scanning probe microscopy thrombophilia |
| url | https://www.reanimatology.com/rmt/article/view/1550 |
| work_keys_str_mv | AT tpbondar spontaneousandinducedplateletaggregationduringpregnancyandlabor AT ayumuratova spontaneousandinducedplateletaggregationduringpregnancyandlabor AT eamelchenko spontaneousandinducedplateletaggregationduringpregnancyandlabor |