Lactobacillus plantarum JCM 1149 Growth Enhancement by using Chlorella sp. KLSc61-pretreated Cells
Dry microalgal biomass was previously tested as prebiotic to enhance the growth of probiotic bacteria. However, the drying process could be ineffective for scaling up probiotic production. This study aimed to investigate the use of fresh, pretreated microalgal biomass to promote the growth of Lactob...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Journal of Pure and Applied Microbiology
2025-06-01
|
| Series: | Journal of Pure and Applied Microbiology |
| Subjects: | |
| Online Access: | https://microbiologyjournal.org/lactobacillus-plantarum-jcm-1149-growth-enhancement-by-using-chlorella-sp-klsc61-pretreated-cells/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849692022394322944 |
|---|---|
| author | Lalita Khanrin Phonwimon Boonyakorn Karn Wongsariya Chokchai Kittiwongwattana Cherdsak Maneeruttanarungroj Kanogkan Leerojanaprapa Wipawee Dejtisakdi |
| author_facet | Lalita Khanrin Phonwimon Boonyakorn Karn Wongsariya Chokchai Kittiwongwattana Cherdsak Maneeruttanarungroj Kanogkan Leerojanaprapa Wipawee Dejtisakdi |
| author_sort | Lalita Khanrin |
| collection | DOAJ |
| description | Dry microalgal biomass was previously tested as prebiotic to enhance the growth of probiotic bacteria. However, the drying process could be ineffective for scaling up probiotic production. This study aimed to investigate the use of fresh, pretreated microalgal biomass to promote the growth of Lactobacillus plantarum JCM 1149. Chlorella sp. KLSc61 cells were pretreated by three different methods: physical treatment with microwave radiation at power levels of 300, 500, and 700 W; chemical treatment with 0.1 M citric acid and 0.5 M sodium hydroxide; and biological treatment with cellulase enzyme. The 2.5% pretreated Chlorella cells were then added to L. plantarum JCM 1149 culture, and the growth was observed at 37 °C for 24 h of incubation. The results showed that, during the log phase (6-10 h), Chlorella cells pretreated with microwave radiation at 700 W were the most effective in promoting L. plantarum JCM 1149 growth, which was 1.4- and 1.5-fold of L. plantarum JCM 1149 without adding Chlorella and with untreated cells, respectively. Extension of the pretreatment time by microwave radiation at 700 W from up to 2 min increased the growth of L. plantarum JCM 1149 up to 1.8-fold of pretreatment time by microwave radiation at 700 W 1 min, compared to the control groups. Additionally, increasing the amount of Chlorella biomass up to 5% (w/v) extended the log phase of L. plantarum JCM 1149 and increased cell accumulation during the stationary phase. Unlike dry microalgal biomass, the simplicity of fresh, pretreated Chlorella biomass shown in this study may facilitate large-scale, commercial production of L. plantarum strains as probiotics. |
| format | Article |
| id | doaj-art-8540a030fc2a4893abf68de2dce424a0 |
| institution | DOAJ |
| issn | 0973-7510 2581-690X |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj-art-8540a030fc2a4893abf68de2dce424a02025-08-20T03:20:51ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2025-06-0119210711084https://doi.org/10.22207/JPAM.19.2.10Lactobacillus plantarum JCM 1149 Growth Enhancement by using Chlorella sp. KLSc61-pretreated CellsLalita Khanrinhttps://orcid.org/0009-0004-8340-0671Phonwimon BoonyakornKarn Wongsariyahttps://orcid.org/0000-0002-8253-5820Chokchai Kittiwongwattanahttps://orcid.org/0000-0002-5574-8580Cherdsak Maneeruttanarungrojhttps://orcid.org/0000-0002-7046-7736Kanogkan Leerojanaprapahttps://orcid.org/0000-0002-9420-9010Wipawee Dejtisakdihttps://orcid.org/0000-0002-0397-2919Dry microalgal biomass was previously tested as prebiotic to enhance the growth of probiotic bacteria. However, the drying process could be ineffective for scaling up probiotic production. This study aimed to investigate the use of fresh, pretreated microalgal biomass to promote the growth of Lactobacillus plantarum JCM 1149. Chlorella sp. KLSc61 cells were pretreated by three different methods: physical treatment with microwave radiation at power levels of 300, 500, and 700 W; chemical treatment with 0.1 M citric acid and 0.5 M sodium hydroxide; and biological treatment with cellulase enzyme. The 2.5% pretreated Chlorella cells were then added to L. plantarum JCM 1149 culture, and the growth was observed at 37 °C for 24 h of incubation. The results showed that, during the log phase (6-10 h), Chlorella cells pretreated with microwave radiation at 700 W were the most effective in promoting L. plantarum JCM 1149 growth, which was 1.4- and 1.5-fold of L. plantarum JCM 1149 without adding Chlorella and with untreated cells, respectively. Extension of the pretreatment time by microwave radiation at 700 W from up to 2 min increased the growth of L. plantarum JCM 1149 up to 1.8-fold of pretreatment time by microwave radiation at 700 W 1 min, compared to the control groups. Additionally, increasing the amount of Chlorella biomass up to 5% (w/v) extended the log phase of L. plantarum JCM 1149 and increased cell accumulation during the stationary phase. Unlike dry microalgal biomass, the simplicity of fresh, pretreated Chlorella biomass shown in this study may facilitate large-scale, commercial production of L. plantarum strains as probiotics.https://microbiologyjournal.org/lactobacillus-plantarum-jcm-1149-growth-enhancement-by-using-chlorella-sp-klsc61-pretreated-cells/chlorella sp. klsc61cell pretreatmentlactobacillus plantarummicroalgae biomass |
| spellingShingle | Lalita Khanrin Phonwimon Boonyakorn Karn Wongsariya Chokchai Kittiwongwattana Cherdsak Maneeruttanarungroj Kanogkan Leerojanaprapa Wipawee Dejtisakdi Lactobacillus plantarum JCM 1149 Growth Enhancement by using Chlorella sp. KLSc61-pretreated Cells Journal of Pure and Applied Microbiology chlorella sp. klsc61 cell pretreatment lactobacillus plantarum microalgae biomass |
| title | Lactobacillus plantarum JCM 1149 Growth Enhancement by using Chlorella sp. KLSc61-pretreated Cells |
| title_full | Lactobacillus plantarum JCM 1149 Growth Enhancement by using Chlorella sp. KLSc61-pretreated Cells |
| title_fullStr | Lactobacillus plantarum JCM 1149 Growth Enhancement by using Chlorella sp. KLSc61-pretreated Cells |
| title_full_unstemmed | Lactobacillus plantarum JCM 1149 Growth Enhancement by using Chlorella sp. KLSc61-pretreated Cells |
| title_short | Lactobacillus plantarum JCM 1149 Growth Enhancement by using Chlorella sp. KLSc61-pretreated Cells |
| title_sort | lactobacillus plantarum jcm 1149 growth enhancement by using chlorella sp klsc61 pretreated cells |
| topic | chlorella sp. klsc61 cell pretreatment lactobacillus plantarum microalgae biomass |
| url | https://microbiologyjournal.org/lactobacillus-plantarum-jcm-1149-growth-enhancement-by-using-chlorella-sp-klsc61-pretreated-cells/ |
| work_keys_str_mv | AT lalitakhanrin lactobacillusplantarumjcm1149growthenhancementbyusingchlorellaspklsc61pretreatedcells AT phonwimonboonyakorn lactobacillusplantarumjcm1149growthenhancementbyusingchlorellaspklsc61pretreatedcells AT karnwongsariya lactobacillusplantarumjcm1149growthenhancementbyusingchlorellaspklsc61pretreatedcells AT chokchaikittiwongwattana lactobacillusplantarumjcm1149growthenhancementbyusingchlorellaspklsc61pretreatedcells AT cherdsakmaneeruttanarungroj lactobacillusplantarumjcm1149growthenhancementbyusingchlorellaspklsc61pretreatedcells AT kanogkanleerojanaprapa lactobacillusplantarumjcm1149growthenhancementbyusingchlorellaspklsc61pretreatedcells AT wipaweedejtisakdi lactobacillusplantarumjcm1149growthenhancementbyusingchlorellaspklsc61pretreatedcells |