Study on the persistence of ciprofloxacin and sulfamethoxazole in simulated drinking water systems

Abstract The antibiotics ciprofloxacin and sulfamethoxazole are well-known to be persistent in drinking water, as they have been detected at the highest concentration and frequency, respectively. These antibiotics persist despite their residence time, water treatment, and environmental conditions en...

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Main Authors: Victoria Rilstone, Yves Filion, Pascale Champagne
Format: Article
Language:English
Published: SpringerOpen 2025-04-01
Series:Environmental Systems Research
Online Access:https://doi.org/10.1186/s40068-025-00396-5
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author Victoria Rilstone
Yves Filion
Pascale Champagne
author_facet Victoria Rilstone
Yves Filion
Pascale Champagne
author_sort Victoria Rilstone
collection DOAJ
description Abstract The antibiotics ciprofloxacin and sulfamethoxazole are well-known to be persistent in drinking water, as they have been detected at the highest concentration and frequency, respectively. These antibiotics persist despite their residence time, water treatment, and environmental conditions encountered in drinking water distribution systems. To better understand this phenomenon, the objectives of this study were to determine their degradation kinetics at a residual, sub-minimum inhibitory concentration while exposed to multi-species biofilms in polyvinyl chloride (PVC) pipe, as well as examine their effect on total cell count (TCC). The results revealed that both antibiotics continued to be detected after the experimental period of 12 days. Ciprofloxacin concentrations decreased by 31.1% (± 3.9%) and 27.4% (± 7.7%) during exposure to the biofilm and PVC-only control respectively, whereas sulfamethoxazole concentrations decreased by 87.2% (± 15.8%) and 3.6% (± 8.6%) during exposure to the biofilm and PVC-only control, respectively. Biofilm TCC increased significantly when exposed to ciprofloxacin (p-value < 0.005), but showed no significant change when exposed to sulfamethoxazole (p-value > 0.05), which suggested that ciprofloxacin enhanced biofilm formation. These results address the gap in antibiotic persistence research by tracing their concentrations, elucidating the mechanisms of sorption and degradation, and discussing their relative implications. As antibiotics continue to persist in drinking water, their interaction with biofilms may contribute to the long-term selection of antibiotic-resistant bacteria, posing potential risks to drinking water safety and public health.
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spelling doaj-art-8533bc79e1e044f68465114f397771c32025-08-20T03:15:12ZengSpringerOpenEnvironmental Systems Research2193-26972025-04-0114111610.1186/s40068-025-00396-5Study on the persistence of ciprofloxacin and sulfamethoxazole in simulated drinking water systemsVictoria Rilstone0Yves Filion1Pascale Champagne2Beaty Water Research Centre, Department of Civil Engineering, Queen’s UniversityBeaty Water Research Centre, Department of Civil Engineering, Queen’s UniversityBeaty Water Research Centre, Department of Civil Engineering, Queen’s UniversityAbstract The antibiotics ciprofloxacin and sulfamethoxazole are well-known to be persistent in drinking water, as they have been detected at the highest concentration and frequency, respectively. These antibiotics persist despite their residence time, water treatment, and environmental conditions encountered in drinking water distribution systems. To better understand this phenomenon, the objectives of this study were to determine their degradation kinetics at a residual, sub-minimum inhibitory concentration while exposed to multi-species biofilms in polyvinyl chloride (PVC) pipe, as well as examine their effect on total cell count (TCC). The results revealed that both antibiotics continued to be detected after the experimental period of 12 days. Ciprofloxacin concentrations decreased by 31.1% (± 3.9%) and 27.4% (± 7.7%) during exposure to the biofilm and PVC-only control respectively, whereas sulfamethoxazole concentrations decreased by 87.2% (± 15.8%) and 3.6% (± 8.6%) during exposure to the biofilm and PVC-only control, respectively. Biofilm TCC increased significantly when exposed to ciprofloxacin (p-value < 0.005), but showed no significant change when exposed to sulfamethoxazole (p-value > 0.05), which suggested that ciprofloxacin enhanced biofilm formation. These results address the gap in antibiotic persistence research by tracing their concentrations, elucidating the mechanisms of sorption and degradation, and discussing their relative implications. As antibiotics continue to persist in drinking water, their interaction with biofilms may contribute to the long-term selection of antibiotic-resistant bacteria, posing potential risks to drinking water safety and public health.https://doi.org/10.1186/s40068-025-00396-5
spellingShingle Victoria Rilstone
Yves Filion
Pascale Champagne
Study on the persistence of ciprofloxacin and sulfamethoxazole in simulated drinking water systems
Environmental Systems Research
title Study on the persistence of ciprofloxacin and sulfamethoxazole in simulated drinking water systems
title_full Study on the persistence of ciprofloxacin and sulfamethoxazole in simulated drinking water systems
title_fullStr Study on the persistence of ciprofloxacin and sulfamethoxazole in simulated drinking water systems
title_full_unstemmed Study on the persistence of ciprofloxacin and sulfamethoxazole in simulated drinking water systems
title_short Study on the persistence of ciprofloxacin and sulfamethoxazole in simulated drinking water systems
title_sort study on the persistence of ciprofloxacin and sulfamethoxazole in simulated drinking water systems
url https://doi.org/10.1186/s40068-025-00396-5
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AT yvesfilion studyonthepersistenceofciprofloxacinandsulfamethoxazoleinsimulateddrinkingwatersystems
AT pascalechampagne studyonthepersistenceofciprofloxacinandsulfamethoxazoleinsimulateddrinkingwatersystems