In Vitro Model for Studying Malignancy Associated Changes
Malignancy associated changes (MAC) can be defined as subtle morphological and physiologic changes that are found in ostensibly normal cells of patients harboring malignant disease. It has been postulated that MAC have a potential to become a useful tool in detection, diagnosis and prognosis of mali...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2003-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2003/238921 |
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| _version_ | 1832555544981798912 |
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| author | Xiao Rong Sun Yonghong Zheng Calum MacAulay Stephen Lam Alexei Doudkine Branko Palcic |
| author_facet | Xiao Rong Sun Yonghong Zheng Calum MacAulay Stephen Lam Alexei Doudkine Branko Palcic |
| author_sort | Xiao Rong Sun |
| collection | DOAJ |
| description | Malignancy associated changes (MAC) can be defined as subtle morphological and physiologic changes that are found in ostensibly normal cells of patients harboring malignant disease. It has been postulated that MAC have a potential to become a useful tool in detection, diagnosis and prognosis of malignant diseases. An in vitro cell culture model system was designed to study interactions between non‐small cell lung cancer (NSCLC) and the normal bronchial epithelium of the human respiratory tract in vivo to see if the MAC‐like phenomenon can be detected in such a system. In this study we examined changes in nuclear features of normal human bronchial epithelial cells (NHBE) when they were co‐cultured with cells derived from a lung cancer cell line NCI‐H460. Using discriminant function analysis, nuclear features were determined which allow maximal discrimination between normal cells incubated with or without cancerous cells. Our results demonstrate that MAC appear to be specific to changes induced by malignancy, and that these changes differ from those induced by growth factors in the serum. This study provides evidence in support to the hypothesis that MAC are induced by a soluble factor(s) released by malignant cells. Colour figure can be viewed on http://www.esacp.org/acp/2003/25‐2/sun.htm. |
| format | Article |
| id | doaj-art-852c990e7fc148668968b44d9b175857 |
| institution | Kabale University |
| issn | 0921-8912 1878-3651 |
| language | English |
| publishDate | 2003-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-852c990e7fc148668968b44d9b1758572025-02-03T05:47:59ZengWileyAnalytical Cellular Pathology0921-89121878-36512003-01-012529510210.1155/2003/238921In Vitro Model for Studying Malignancy Associated ChangesXiao Rong Sun0Yonghong Zheng1Calum MacAulay2Stephen Lam3Alexei Doudkine4Branko Palcic5Cancer Imaging Department, British Columbia Cancer Agency, Vancouver, CanadaCancer Imaging Department, British Columbia Cancer Agency, Vancouver, CanadaCancer Imaging Department, British Columbia Cancer Agency, Vancouver, CanadaCancer Imaging Department, British Columbia Cancer Agency, Vancouver, CanadaCancer Imaging Department, British Columbia Cancer Agency, Vancouver, CanadaCancer Imaging Department, British Columbia Cancer Agency, Vancouver, CanadaMalignancy associated changes (MAC) can be defined as subtle morphological and physiologic changes that are found in ostensibly normal cells of patients harboring malignant disease. It has been postulated that MAC have a potential to become a useful tool in detection, diagnosis and prognosis of malignant diseases. An in vitro cell culture model system was designed to study interactions between non‐small cell lung cancer (NSCLC) and the normal bronchial epithelium of the human respiratory tract in vivo to see if the MAC‐like phenomenon can be detected in such a system. In this study we examined changes in nuclear features of normal human bronchial epithelial cells (NHBE) when they were co‐cultured with cells derived from a lung cancer cell line NCI‐H460. Using discriminant function analysis, nuclear features were determined which allow maximal discrimination between normal cells incubated with or without cancerous cells. Our results demonstrate that MAC appear to be specific to changes induced by malignancy, and that these changes differ from those induced by growth factors in the serum. This study provides evidence in support to the hypothesis that MAC are induced by a soluble factor(s) released by malignant cells. Colour figure can be viewed on http://www.esacp.org/acp/2003/25‐2/sun.htm.http://dx.doi.org/10.1155/2003/238921 |
| spellingShingle | Xiao Rong Sun Yonghong Zheng Calum MacAulay Stephen Lam Alexei Doudkine Branko Palcic In Vitro Model for Studying Malignancy Associated Changes Analytical Cellular Pathology |
| title | In Vitro Model for Studying Malignancy Associated Changes |
| title_full | In Vitro Model for Studying Malignancy Associated Changes |
| title_fullStr | In Vitro Model for Studying Malignancy Associated Changes |
| title_full_unstemmed | In Vitro Model for Studying Malignancy Associated Changes |
| title_short | In Vitro Model for Studying Malignancy Associated Changes |
| title_sort | in vitro model for studying malignancy associated changes |
| url | http://dx.doi.org/10.1155/2003/238921 |
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