Effectiveness of katuk leaf chlorophyll (Sauropus androgynus (L) Merr) with blue and red laser a ctivation to reduce Aggregatibacter actinomycetemcomitans and Enterococcus faecalis biofilm
In this study, the efficacy of using Sauropus androgynus (L) Merr, a katuk leaf chlorophyll photosensitizer, to reduce Aggregatibacter actinomycetemcomitans and Enterococcus faecalis biofilm was investigated. A red and blue diode laser is used as the light source. The sample was split into four grou...
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| Format: | Article |
| Language: | Russian |
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Non-profit partnership for development of domestic photodynamic therapy and photodiagnosis "Russian Photodynamic Association"
2023-05-01
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| Series: | Biomedical Photonics |
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| Online Access: | https://www.pdt-journal.com/jour/article/view/579 |
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| author | P. A. D. Permatasari S. D. Astuti A. K. Yaqubi E. A. W. Paisei . Pujiyanto Nasrul Anuar |
| author_facet | P. A. D. Permatasari S. D. Astuti A. K. Yaqubi E. A. W. Paisei . Pujiyanto Nasrul Anuar |
| author_sort | P. A. D. Permatasari |
| collection | DOAJ |
| description | In this study, the efficacy of using Sauropus androgynus (L) Merr, a katuk leaf chlorophyll photosensitizer, to reduce Aggregatibacter actinomycetemcomitans and Enterococcus faecalis biofilm was investigated. A red and blue diode laser is used as the light source. The sample was split into four groups: a negative control group, a positive control group, a blue laser treatment group (B), and a red laser treatment group (R), both with and without the addition of katuk leaf chlorophyll 1.6 mg/ml, and with varying densities of laser energy exposure of 2.5 J/cm2, 5 J/cm2, 7.5 J/cm2, and 10 J/cm2. Laser exposure and chlorophyll photosensitizer were tested using ELISA and ANOVA. At an energy density of 10 J/cm2, the optimal bacterial mortality rate was obtained in each treatment group. Namely, in the Aggregatibacter actinomycetemcomitans biofilm, the negative group, the number of deaths was 73.30% using a blue diode laser and 63.25% using a red diode laser. In the positive group, the number of deaths was 86.12% using a blue diode laser and 83.29% using a red diode laser. In the Enterococcus faecalis biofilm, in the negative group, the number of deaths was 67.78% using the blue diode laser and 75.33% using the red diode laser, and in the positive group, the number of deaths was 71.71% using the blue diode laser and 86.41 using a red diode laser. Exposure to blue and red diode lasers activates chlorophyll in katuk leaves, killing bacteria and reducing biofilms. |
| format | Article |
| id | doaj-art-851cade5c7f84eb4ab966c4a907e835e |
| institution | Kabale University |
| issn | 2413-9432 |
| language | Russian |
| publishDate | 2023-05-01 |
| publisher | Non-profit partnership for development of domestic photodynamic therapy and photodiagnosis "Russian Photodynamic Association" |
| record_format | Article |
| series | Biomedical Photonics |
| spelling | doaj-art-851cade5c7f84eb4ab966c4a907e835e2025-08-20T03:35:29ZrusNon-profit partnership for development of domestic photodynamic therapy and photodiagnosis "Russian Photodynamic Association"Biomedical Photonics2413-94322023-05-01121142110.24931/2413-9432-2023-12-1-14-21268Effectiveness of katuk leaf chlorophyll (Sauropus androgynus (L) Merr) with blue and red laser a ctivation to reduce Aggregatibacter actinomycetemcomitans and Enterococcus faecalis biofilmP. A. D. Permatasari0S. D. Astuti1A. K. Yaqubi2E. A. W. Paisei3. Pujiyanto4Nasrul Anuar5Airlangga UniversityAirlangga UniversityAirlangga UniversityAirlangga UniversityAirlangga UniversityUniversiti MalayaIn this study, the efficacy of using Sauropus androgynus (L) Merr, a katuk leaf chlorophyll photosensitizer, to reduce Aggregatibacter actinomycetemcomitans and Enterococcus faecalis biofilm was investigated. A red and blue diode laser is used as the light source. The sample was split into four groups: a negative control group, a positive control group, a blue laser treatment group (B), and a red laser treatment group (R), both with and without the addition of katuk leaf chlorophyll 1.6 mg/ml, and with varying densities of laser energy exposure of 2.5 J/cm2, 5 J/cm2, 7.5 J/cm2, and 10 J/cm2. Laser exposure and chlorophyll photosensitizer were tested using ELISA and ANOVA. At an energy density of 10 J/cm2, the optimal bacterial mortality rate was obtained in each treatment group. Namely, in the Aggregatibacter actinomycetemcomitans biofilm, the negative group, the number of deaths was 73.30% using a blue diode laser and 63.25% using a red diode laser. In the positive group, the number of deaths was 86.12% using a blue diode laser and 83.29% using a red diode laser. In the Enterococcus faecalis biofilm, in the negative group, the number of deaths was 67.78% using the blue diode laser and 75.33% using the red diode laser, and in the positive group, the number of deaths was 71.71% using the blue diode laser and 86.41 using a red diode laser. Exposure to blue and red diode lasers activates chlorophyll in katuk leaves, killing bacteria and reducing biofilms.https://www.pdt-journal.com/jour/article/view/579photoinactivationblue and red diode laserkatuk leaf chlorophyll (sauropus androgynus (l) merr)aggregatibacter actinomycetemcomitansenterococcus faecalis |
| spellingShingle | P. A. D. Permatasari S. D. Astuti A. K. Yaqubi E. A. W. Paisei . Pujiyanto Nasrul Anuar Effectiveness of katuk leaf chlorophyll (Sauropus androgynus (L) Merr) with blue and red laser a ctivation to reduce Aggregatibacter actinomycetemcomitans and Enterococcus faecalis biofilm Biomedical Photonics photoinactivation blue and red diode laser katuk leaf chlorophyll (sauropus androgynus (l) merr) aggregatibacter actinomycetemcomitans enterococcus faecalis |
| title | Effectiveness of katuk leaf chlorophyll (Sauropus androgynus (L) Merr) with blue and red laser a ctivation to reduce Aggregatibacter actinomycetemcomitans and Enterococcus faecalis biofilm |
| title_full | Effectiveness of katuk leaf chlorophyll (Sauropus androgynus (L) Merr) with blue and red laser a ctivation to reduce Aggregatibacter actinomycetemcomitans and Enterococcus faecalis biofilm |
| title_fullStr | Effectiveness of katuk leaf chlorophyll (Sauropus androgynus (L) Merr) with blue and red laser a ctivation to reduce Aggregatibacter actinomycetemcomitans and Enterococcus faecalis biofilm |
| title_full_unstemmed | Effectiveness of katuk leaf chlorophyll (Sauropus androgynus (L) Merr) with blue and red laser a ctivation to reduce Aggregatibacter actinomycetemcomitans and Enterococcus faecalis biofilm |
| title_short | Effectiveness of katuk leaf chlorophyll (Sauropus androgynus (L) Merr) with blue and red laser a ctivation to reduce Aggregatibacter actinomycetemcomitans and Enterococcus faecalis biofilm |
| title_sort | effectiveness of katuk leaf chlorophyll sauropus androgynus l merr with blue and red laser a ctivation to reduce aggregatibacter actinomycetemcomitans and enterococcus faecalis biofilm |
| topic | photoinactivation blue and red diode laser katuk leaf chlorophyll (sauropus androgynus (l) merr) aggregatibacter actinomycetemcomitans enterococcus faecalis |
| url | https://www.pdt-journal.com/jour/article/view/579 |
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