Immunohistochemical Staining Properties of Osteopontin and Ki-67 in Feline Meningiomas
The high recurrence rate of feline meningioma despite the generally benign histomorphology warrants additional markers of clinical aggressiveness. The Ki-67 index is commonly used as prognostic marker for meningioma recurrence in people. Osteopontin (OPN) is a protein involved in tumor progression a...
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2024-11-01
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| author | Gabriele Gradner Janina Janssen Anna Oevermann Alexander Tichy Stefan Kummer Stefanie Burger Ingrid Walter |
| author_facet | Gabriele Gradner Janina Janssen Anna Oevermann Alexander Tichy Stefan Kummer Stefanie Burger Ingrid Walter |
| author_sort | Gabriele Gradner |
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| description | The high recurrence rate of feline meningioma despite the generally benign histomorphology warrants additional markers of clinical aggressiveness. The Ki-67 index is commonly used as prognostic marker for meningioma recurrence in people. Osteopontin (OPN) is a protein involved in tumor progression and may be a potential malignancy marker. To date, osteopontin expression has not been investigated in feline meningioma. The aim of this study was to evaluate the extent of Ki-67 and osteopontin immunostaining of feline meningioma and to find possible associations with WHO (World Health Organization) grades and subtypes. Fifty-three feline meningioma samples were graded according to the human WHO classification and underwent immunohistochemical examination for Ki-67 and OPN. Fifty samples were classified as WHO grade I and three as WHO grade II. The mean Ki-67 ratio was 9.19 ± 9.47. Osteopontin expression was correspondingly high with a mean OPN IHC score of 150.17 (0–242.8), and a median Allred score of 7 (0–8). There was no significant correlation with Ki-67 index, osteopontin expression, WHO grades, or subtypes. The overall high expressions of osteopontin and Ki-67 may help explain the tendency for recurrence of feline meningioma. The human WHO grading system may not be sufficient to accurately estimate the clinical behavior of meningioma in this species. |
| format | Article |
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| language | English |
| publishDate | 2024-11-01 |
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| spelling | doaj-art-84fc1becc9df4a9d81e36296b9cc22472025-08-20T02:38:38ZengMDPI AGAnimals2076-26152024-11-011423340410.3390/ani14233404Immunohistochemical Staining Properties of Osteopontin and Ki-67 in Feline MeningiomasGabriele Gradner0Janina Janssen1Anna Oevermann2Alexander Tichy3Stefan Kummer4Stefanie Burger5Ingrid Walter6Small Animal Surgery Unit, University of Veterinary Medicine, 1210 Veterinaerplatz 1, 1210 Vienna, AustriaSmall Animal Surgery Unit, University of Veterinary Medicine, 1210 Veterinaerplatz 1, 1210 Vienna, AustriaDepartment of Clinical Research and Veterinary Public Health, University of Veterinary Medicine, 3001 Bremgartenstrasse 109 a, 3014 Bern, SwitzerlandPlatform Bioinformatic and Biostatistics, University of Veterinary Medicine, 1210 Veterinaerplatz 1, 1210 Vienna, AustriaVetCore Facility for Research, University of Veterinary Medicine, 1210 Veterinaerplatz 1, 1210 Vienna, AustriaVetCore Facility for Research, University of Veterinary Medicine, 1210 Veterinaerplatz 1, 1210 Vienna, AustriaVetCore Facility for Research, University of Veterinary Medicine, 1210 Veterinaerplatz 1, 1210 Vienna, AustriaThe high recurrence rate of feline meningioma despite the generally benign histomorphology warrants additional markers of clinical aggressiveness. The Ki-67 index is commonly used as prognostic marker for meningioma recurrence in people. Osteopontin (OPN) is a protein involved in tumor progression and may be a potential malignancy marker. To date, osteopontin expression has not been investigated in feline meningioma. The aim of this study was to evaluate the extent of Ki-67 and osteopontin immunostaining of feline meningioma and to find possible associations with WHO (World Health Organization) grades and subtypes. Fifty-three feline meningioma samples were graded according to the human WHO classification and underwent immunohistochemical examination for Ki-67 and OPN. Fifty samples were classified as WHO grade I and three as WHO grade II. The mean Ki-67 ratio was 9.19 ± 9.47. Osteopontin expression was correspondingly high with a mean OPN IHC score of 150.17 (0–242.8), and a median Allred score of 7 (0–8). There was no significant correlation with Ki-67 index, osteopontin expression, WHO grades, or subtypes. The overall high expressions of osteopontin and Ki-67 may help explain the tendency for recurrence of feline meningioma. The human WHO grading system may not be sufficient to accurately estimate the clinical behavior of meningioma in this species.https://www.mdpi.com/2076-2615/14/23/3404osteopontinKi-67immunohistochemistrycatsmeningiomaWHO |
| spellingShingle | Gabriele Gradner Janina Janssen Anna Oevermann Alexander Tichy Stefan Kummer Stefanie Burger Ingrid Walter Immunohistochemical Staining Properties of Osteopontin and Ki-67 in Feline Meningiomas Animals osteopontin Ki-67 immunohistochemistry cats meningioma WHO |
| title | Immunohistochemical Staining Properties of Osteopontin and Ki-67 in Feline Meningiomas |
| title_full | Immunohistochemical Staining Properties of Osteopontin and Ki-67 in Feline Meningiomas |
| title_fullStr | Immunohistochemical Staining Properties of Osteopontin and Ki-67 in Feline Meningiomas |
| title_full_unstemmed | Immunohistochemical Staining Properties of Osteopontin and Ki-67 in Feline Meningiomas |
| title_short | Immunohistochemical Staining Properties of Osteopontin and Ki-67 in Feline Meningiomas |
| title_sort | immunohistochemical staining properties of osteopontin and ki 67 in feline meningiomas |
| topic | osteopontin Ki-67 immunohistochemistry cats meningioma WHO |
| url | https://www.mdpi.com/2076-2615/14/23/3404 |
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