Effect of castration and days on feed on body composition, glucose tolerance, and muscle gene expression in Nellore cattle
ABSTRACT The objective of this study was to evaluate the effect of castration and days of feed (DOF) on glucose sensitivity and muscle expression of genes related to glucose metabolism, muscle growth, and fat deposition. Thirty-six Nellore bull calves were either surgically castrated or left intact,...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sociedade Brasileira de Zootecnia
2025-08-01
|
| Series: | Revista Brasileira de Zootecnia |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982025000101106&lng=en&tlng=en |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849335853591035904 |
|---|---|
| author | Luiz Henrique Pereira Silva Germán Darío Ramírez-Zamudio Walmir da Silva Débora Evelyn de Freitas Assis Gutierrez José de Freitas Assis Mauricio Miguel Estrada Simone Eliza Facioni Guimarães Marcio de Souza Duarte Carl Robertson Dahlen Mario Luiz Chizzotti |
| author_facet | Luiz Henrique Pereira Silva Germán Darío Ramírez-Zamudio Walmir da Silva Débora Evelyn de Freitas Assis Gutierrez José de Freitas Assis Mauricio Miguel Estrada Simone Eliza Facioni Guimarães Marcio de Souza Duarte Carl Robertson Dahlen Mario Luiz Chizzotti |
| author_sort | Luiz Henrique Pereira Silva |
| collection | DOAJ |
| description | ABSTRACT The objective of this study was to evaluate the effect of castration and days of feed (DOF) on glucose sensitivity and muscle expression of genes related to glucose metabolism, muscle growth, and fat deposition. Thirty-six Nellore bull calves were either surgically castrated or left intact, then harvested at 0, 100, or 200 DOF. Glucose tolerance tests were performed at 100 and 200 DOF. Longissimus dorsi (LD) samples were taken just after stunning for gene expression. Data were analyzed as a complete randomized design following a two (sexual condition) × three (DOF) factorial arrangement of treatments. An interaction effect was found for carcass fat gain (P = 0.01), showing that steers gained more fat than bulls from 100 to 200 DOF, but both sexual classes had a similar fat gain from 0 to 100 DOF. Basal blood glucose and the area under the curve post-infusion were not affected by castration or DOF (P>0.05). Castration tended (P = 0.08) to upregulate LD expression of Acetyl-CoA carboxylase alpha (ACACA). Interaction effects (P<0.05) were found for LD expression of IGF-1 receptor (IGF1R) and F-box protein 32 (FBXO32) with steers having greater relative abundance for both genes at 100 DOF, while bulls had greater relative abundance at 200 DOF. Despite the greater carcass fattening enhancement by castration and longer DOF, whole-body sensitivity to glucose does not change. |
| format | Article |
| id | doaj-art-84fb6e1a8b3d4dec94ccf2310dc9461a |
| institution | Kabale University |
| issn | 1806-9290 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Sociedade Brasileira de Zootecnia |
| record_format | Article |
| series | Revista Brasileira de Zootecnia |
| spelling | doaj-art-84fb6e1a8b3d4dec94ccf2310dc9461a2025-08-20T03:45:08ZengSociedade Brasileira de ZootecniaRevista Brasileira de Zootecnia1806-92902025-08-015410.37496/rbz5420250001Effect of castration and days on feed on body composition, glucose tolerance, and muscle gene expression in Nellore cattleLuiz Henrique Pereira Silvahttps://orcid.org/0000-0003-1447-1674Germán Darío Ramírez-Zamudiohttps://orcid.org/0000-0002-4843-7653Walmir da Silvahttps://orcid.org/0000-0003-3188-8076Débora Evelyn de Freitas Assishttps://orcid.org/0009-0000-1663-0590Gutierrez José de Freitas Assishttps://orcid.org/0000-0002-0156-9376Mauricio Miguel Estradahttps://orcid.org/0000-0002-6830-4399Simone Eliza Facioni Guimarãeshttps://orcid.org/0000-0003-3704-8131Marcio de Souza Duartehttps://orcid.org/0000-0002-5795-6420Carl Robertson Dahlenhttps://orcid.org/0000-0002-4556-4315Mario Luiz Chizzottihttps://orcid.org/0000-0001-9770-6891ABSTRACT The objective of this study was to evaluate the effect of castration and days of feed (DOF) on glucose sensitivity and muscle expression of genes related to glucose metabolism, muscle growth, and fat deposition. Thirty-six Nellore bull calves were either surgically castrated or left intact, then harvested at 0, 100, or 200 DOF. Glucose tolerance tests were performed at 100 and 200 DOF. Longissimus dorsi (LD) samples were taken just after stunning for gene expression. Data were analyzed as a complete randomized design following a two (sexual condition) × three (DOF) factorial arrangement of treatments. An interaction effect was found for carcass fat gain (P = 0.01), showing that steers gained more fat than bulls from 100 to 200 DOF, but both sexual classes had a similar fat gain from 0 to 100 DOF. Basal blood glucose and the area under the curve post-infusion were not affected by castration or DOF (P>0.05). Castration tended (P = 0.08) to upregulate LD expression of Acetyl-CoA carboxylase alpha (ACACA). Interaction effects (P<0.05) were found for LD expression of IGF-1 receptor (IGF1R) and F-box protein 32 (FBXO32) with steers having greater relative abundance for both genes at 100 DOF, while bulls had greater relative abundance at 200 DOF. Despite the greater carcass fattening enhancement by castration and longer DOF, whole-body sensitivity to glucose does not change.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982025000101106&lng=en&tlng=encarcass compositioncastrationcattleglucose tolerance test |
| spellingShingle | Luiz Henrique Pereira Silva Germán Darío Ramírez-Zamudio Walmir da Silva Débora Evelyn de Freitas Assis Gutierrez José de Freitas Assis Mauricio Miguel Estrada Simone Eliza Facioni Guimarães Marcio de Souza Duarte Carl Robertson Dahlen Mario Luiz Chizzotti Effect of castration and days on feed on body composition, glucose tolerance, and muscle gene expression in Nellore cattle Revista Brasileira de Zootecnia carcass composition castration cattle glucose tolerance test |
| title | Effect of castration and days on feed on body composition, glucose tolerance, and muscle gene expression in Nellore cattle |
| title_full | Effect of castration and days on feed on body composition, glucose tolerance, and muscle gene expression in Nellore cattle |
| title_fullStr | Effect of castration and days on feed on body composition, glucose tolerance, and muscle gene expression in Nellore cattle |
| title_full_unstemmed | Effect of castration and days on feed on body composition, glucose tolerance, and muscle gene expression in Nellore cattle |
| title_short | Effect of castration and days on feed on body composition, glucose tolerance, and muscle gene expression in Nellore cattle |
| title_sort | effect of castration and days on feed on body composition glucose tolerance and muscle gene expression in nellore cattle |
| topic | carcass composition castration cattle glucose tolerance test |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982025000101106&lng=en&tlng=en |
| work_keys_str_mv | AT luizhenriquepereirasilva effectofcastrationanddaysonfeedonbodycompositionglucosetoleranceandmusclegeneexpressioninnellorecattle AT germandarioramirezzamudio effectofcastrationanddaysonfeedonbodycompositionglucosetoleranceandmusclegeneexpressioninnellorecattle AT walmirdasilva effectofcastrationanddaysonfeedonbodycompositionglucosetoleranceandmusclegeneexpressioninnellorecattle AT deboraevelyndefreitasassis effectofcastrationanddaysonfeedonbodycompositionglucosetoleranceandmusclegeneexpressioninnellorecattle AT gutierrezjosedefreitasassis effectofcastrationanddaysonfeedonbodycompositionglucosetoleranceandmusclegeneexpressioninnellorecattle AT mauriciomiguelestrada effectofcastrationanddaysonfeedonbodycompositionglucosetoleranceandmusclegeneexpressioninnellorecattle AT simoneelizafacioniguimaraes effectofcastrationanddaysonfeedonbodycompositionglucosetoleranceandmusclegeneexpressioninnellorecattle AT marciodesouzaduarte effectofcastrationanddaysonfeedonbodycompositionglucosetoleranceandmusclegeneexpressioninnellorecattle AT carlrobertsondahlen effectofcastrationanddaysonfeedonbodycompositionglucosetoleranceandmusclegeneexpressioninnellorecattle AT marioluizchizzotti effectofcastrationanddaysonfeedonbodycompositionglucosetoleranceandmusclegeneexpressioninnellorecattle |