Artesunate improves cryopreserved yak sperm quality by activating SOD1 antioxidant pathway
IntroductionSperm damage from oxidative stress is common in semen cryopreservation. Natural antioxidants for frozen semen are largely unexplored. Artesunate (ART), derived from Artemisia annua and used for malaria treatment, shows potential as a frozen semen antioxidant.MethodsWe investigated the ef...
Saved in:
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-06-01
|
| Series: | Frontiers in Veterinary Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2025.1613506/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849420008359198720 |
|---|---|
| author | Yilin Fan Yilin Fan Weibing Lv Weibing Lv Xiaowei Li Geri Wanma Geri Wanma Jian Li Jian Li Xianrong Xiong Xianrong Xiong Xiaoqiang He Yanwen Wang Shi Yin Shi Yin Wei Fu Wei Fu Honghong He Honghong He Yaqiu Lin Yaqiu Lin Daoliang Lan Daoliang Lan Yan Xiong Yan Xiong |
| author_facet | Yilin Fan Yilin Fan Weibing Lv Weibing Lv Xiaowei Li Geri Wanma Geri Wanma Jian Li Jian Li Xianrong Xiong Xianrong Xiong Xiaoqiang He Yanwen Wang Shi Yin Shi Yin Wei Fu Wei Fu Honghong He Honghong He Yaqiu Lin Yaqiu Lin Daoliang Lan Daoliang Lan Yan Xiong Yan Xiong |
| author_sort | Yilin Fan |
| collection | DOAJ |
| description | IntroductionSperm damage from oxidative stress is common in semen cryopreservation. Natural antioxidants for frozen semen are largely unexplored. Artesunate (ART), derived from Artemisia annua and used for malaria treatment, shows potential as a frozen semen antioxidant.MethodsWe investigated the effects of various ART concentrations (0.2, 0.5, and 1.0 mM) on yak semen cryopreservation and examined the molecular mechanisms involved.ResultsPost-freezing, sperm quality markedly declined. However, 0.2 mM ART significantly improved sperm motility (both total and progressive) and reduced membrane damage. ART treatment effectively alleviated oxidative stress by reducing ROS levels and MDA content in the sperm while selectively upregulating SOD1 activity and protein levels, which are involved in maintaining DNA integrity and mitochondrial function.ConclusionThese findings support the hypothesis that ART exerts antioxidant effects via a SOD1-mediated mechanism, highlighting its potential to improve frozen semen quality and offering a novel strategy for cryopreserving sperm in other species. |
| format | Article |
| id | doaj-art-84f76db14ba54301bda7764dea81aabc |
| institution | Kabale University |
| issn | 2297-1769 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj-art-84f76db14ba54301bda7764dea81aabc2025-08-20T03:31:53ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-06-011210.3389/fvets.2025.16135061613506Artesunate improves cryopreserved yak sperm quality by activating SOD1 antioxidant pathwayYilin Fan0Yilin Fan1Weibing Lv2Weibing Lv3Xiaowei Li4Geri Wanma5Geri Wanma6Jian Li7Jian Li8Xianrong Xiong9Xianrong Xiong10Xiaoqiang He11Yanwen Wang12Shi Yin13Shi Yin14Wei Fu15Wei Fu16Honghong He17Honghong He18Yaqiu Lin19Yaqiu Lin20Daoliang Lan21Daoliang Lan22Yan Xiong23Yan Xiong24Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Reservation and Utilization, Ministry of Education and Sichuan Province, Southwest Minzu University, Chengdu, ChinaKey Laboratory of Animal Science of National Ethnic Affairs Commission of China, Southwest Minzu University, Chengdu, ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Reservation and Utilization, Ministry of Education and Sichuan Province, Southwest Minzu University, Chengdu, ChinaKey Laboratory of Animal Science of National Ethnic Affairs Commission of China, Southwest Minzu University, Chengdu, ChinaLongri Breeding Stock Farm of Sichuan Province, Dujiangyan, ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Reservation and Utilization, Ministry of Education and Sichuan Province, Southwest Minzu University, Chengdu, ChinaKey Laboratory of Animal Science of National Ethnic Affairs Commission of China, Southwest Minzu University, Chengdu, ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Reservation and Utilization, Ministry of Education and Sichuan Province, Southwest Minzu University, Chengdu, ChinaKey Laboratory of Animal Science of National Ethnic Affairs Commission of China, Southwest Minzu University, Chengdu, ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Reservation and Utilization, Ministry of Education and Sichuan Province, Southwest Minzu University, Chengdu, ChinaKey Laboratory of Animal Science of National Ethnic Affairs Commission of China, Southwest Minzu University, Chengdu, ChinaLongri Breeding Stock Farm of Sichuan Province, Dujiangyan, ChinaLongri Breeding Stock Farm of Sichuan Province, Dujiangyan, ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Reservation and Utilization, Ministry of Education and Sichuan Province, Southwest Minzu University, Chengdu, ChinaKey Laboratory of Animal Science of National Ethnic Affairs Commission of China, Southwest Minzu University, Chengdu, ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Reservation and Utilization, Ministry of Education and Sichuan Province, Southwest Minzu University, Chengdu, ChinaKey Laboratory of Animal Science of National Ethnic Affairs Commission of China, Southwest Minzu University, Chengdu, ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Reservation and Utilization, Ministry of Education and Sichuan Province, Southwest Minzu University, Chengdu, ChinaKey Laboratory of Animal Science of National Ethnic Affairs Commission of China, Southwest Minzu University, Chengdu, ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Reservation and Utilization, Ministry of Education and Sichuan Province, Southwest Minzu University, Chengdu, ChinaKey Laboratory of Animal Science of National Ethnic Affairs Commission of China, Southwest Minzu University, Chengdu, ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Reservation and Utilization, Ministry of Education and Sichuan Province, Southwest Minzu University, Chengdu, ChinaKey Laboratory of Animal Science of National Ethnic Affairs Commission of China, Southwest Minzu University, Chengdu, ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Reservation and Utilization, Ministry of Education and Sichuan Province, Southwest Minzu University, Chengdu, ChinaKey Laboratory of Animal Science of National Ethnic Affairs Commission of China, Southwest Minzu University, Chengdu, ChinaIntroductionSperm damage from oxidative stress is common in semen cryopreservation. Natural antioxidants for frozen semen are largely unexplored. Artesunate (ART), derived from Artemisia annua and used for malaria treatment, shows potential as a frozen semen antioxidant.MethodsWe investigated the effects of various ART concentrations (0.2, 0.5, and 1.0 mM) on yak semen cryopreservation and examined the molecular mechanisms involved.ResultsPost-freezing, sperm quality markedly declined. However, 0.2 mM ART significantly improved sperm motility (both total and progressive) and reduced membrane damage. ART treatment effectively alleviated oxidative stress by reducing ROS levels and MDA content in the sperm while selectively upregulating SOD1 activity and protein levels, which are involved in maintaining DNA integrity and mitochondrial function.ConclusionThese findings support the hypothesis that ART exerts antioxidant effects via a SOD1-mediated mechanism, highlighting its potential to improve frozen semen quality and offering a novel strategy for cryopreserving sperm in other species.https://www.frontiersin.org/articles/10.3389/fvets.2025.1613506/fullyak spermcryopreservationartesunateSOD1 proteinantioxidant |
| spellingShingle | Yilin Fan Yilin Fan Weibing Lv Weibing Lv Xiaowei Li Geri Wanma Geri Wanma Jian Li Jian Li Xianrong Xiong Xianrong Xiong Xiaoqiang He Yanwen Wang Shi Yin Shi Yin Wei Fu Wei Fu Honghong He Honghong He Yaqiu Lin Yaqiu Lin Daoliang Lan Daoliang Lan Yan Xiong Yan Xiong Artesunate improves cryopreserved yak sperm quality by activating SOD1 antioxidant pathway Frontiers in Veterinary Science yak sperm cryopreservation artesunate SOD1 protein antioxidant |
| title | Artesunate improves cryopreserved yak sperm quality by activating SOD1 antioxidant pathway |
| title_full | Artesunate improves cryopreserved yak sperm quality by activating SOD1 antioxidant pathway |
| title_fullStr | Artesunate improves cryopreserved yak sperm quality by activating SOD1 antioxidant pathway |
| title_full_unstemmed | Artesunate improves cryopreserved yak sperm quality by activating SOD1 antioxidant pathway |
| title_short | Artesunate improves cryopreserved yak sperm quality by activating SOD1 antioxidant pathway |
| title_sort | artesunate improves cryopreserved yak sperm quality by activating sod1 antioxidant pathway |
| topic | yak sperm cryopreservation artesunate SOD1 protein antioxidant |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2025.1613506/full |
| work_keys_str_mv | AT yilinfan artesunateimprovescryopreservedyakspermqualitybyactivatingsod1antioxidantpathway AT yilinfan artesunateimprovescryopreservedyakspermqualitybyactivatingsod1antioxidantpathway AT weibinglv artesunateimprovescryopreservedyakspermqualitybyactivatingsod1antioxidantpathway AT weibinglv artesunateimprovescryopreservedyakspermqualitybyactivatingsod1antioxidantpathway AT xiaoweili artesunateimprovescryopreservedyakspermqualitybyactivatingsod1antioxidantpathway AT geriwanma artesunateimprovescryopreservedyakspermqualitybyactivatingsod1antioxidantpathway AT geriwanma artesunateimprovescryopreservedyakspermqualitybyactivatingsod1antioxidantpathway AT jianli artesunateimprovescryopreservedyakspermqualitybyactivatingsod1antioxidantpathway AT jianli artesunateimprovescryopreservedyakspermqualitybyactivatingsod1antioxidantpathway AT xianrongxiong artesunateimprovescryopreservedyakspermqualitybyactivatingsod1antioxidantpathway AT xianrongxiong artesunateimprovescryopreservedyakspermqualitybyactivatingsod1antioxidantpathway AT xiaoqianghe artesunateimprovescryopreservedyakspermqualitybyactivatingsod1antioxidantpathway AT yanwenwang artesunateimprovescryopreservedyakspermqualitybyactivatingsod1antioxidantpathway AT shiyin artesunateimprovescryopreservedyakspermqualitybyactivatingsod1antioxidantpathway AT shiyin artesunateimprovescryopreservedyakspermqualitybyactivatingsod1antioxidantpathway AT weifu artesunateimprovescryopreservedyakspermqualitybyactivatingsod1antioxidantpathway AT weifu artesunateimprovescryopreservedyakspermqualitybyactivatingsod1antioxidantpathway AT honghonghe artesunateimprovescryopreservedyakspermqualitybyactivatingsod1antioxidantpathway AT honghonghe artesunateimprovescryopreservedyakspermqualitybyactivatingsod1antioxidantpathway AT yaqiulin artesunateimprovescryopreservedyakspermqualitybyactivatingsod1antioxidantpathway AT yaqiulin artesunateimprovescryopreservedyakspermqualitybyactivatingsod1antioxidantpathway AT daolianglan artesunateimprovescryopreservedyakspermqualitybyactivatingsod1antioxidantpathway AT daolianglan artesunateimprovescryopreservedyakspermqualitybyactivatingsod1antioxidantpathway AT yanxiong artesunateimprovescryopreservedyakspermqualitybyactivatingsod1antioxidantpathway AT yanxiong artesunateimprovescryopreservedyakspermqualitybyactivatingsod1antioxidantpathway |