Direct Amplification and Cloning of Up to 5-kb Lentivirus Genomes from Serum
To produce large cDNA strands from biological samples containing limited numbers of template molecules, it may be necessary to minimize both nonspecific primer attachment in first-strand synthesis and secondary structure in RNA molecules. Failure to do so could result in the accumulation of shortene...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1996-08-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/96212rr06 |
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