Anti-Aging Effects and Mechanisms of Cod Collagen Peptides (CCPs) in <i>Caenorhabditis elegans</i>
Given the growing interest in natural compounds for promoting healthy aging, this study aimed to investigate the potential of cod collagen peptides (CCPs), a readily available marine resource, to extend lifespan and improve health. Lifespan assays were performed on <i>C. elegans</i> trea...
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2025-04-01
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| Series: | Journal of Functional Biomaterials |
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| author | Jiale Wei Junjie Zhang Nan Ding Yu Liu Yuzhen Wu Rui Duan |
| author_facet | Jiale Wei Junjie Zhang Nan Ding Yu Liu Yuzhen Wu Rui Duan |
| author_sort | Jiale Wei |
| collection | DOAJ |
| description | Given the growing interest in natural compounds for promoting healthy aging, this study aimed to investigate the potential of cod collagen peptides (CCPs), a readily available marine resource, to extend lifespan and improve health. Lifespan assays were performed on <i>C. elegans</i> treated with different concentrations of CCPs. Furthermore, various stress resistance assays, including those evaluating oxidative and thermal stress, were conducted. To elucidate the underlying mechanisms, gene expression analysis of key aging-related genes was performed. The results demonstrated that treatment with 25 mg/mL of CCPs extended the lifespan of <i>C. elegans</i> by 13.2%, increased body length and width by 14.8% and 20.6%, respectively, and enhanced head-swing and body-bending frequencies by 66.9% and 80.4%. Lipofuscin content and apoptosis were reduced by 45.9% and 34.1%, respectively. <i>C. elegans</i> treated with 25 mg/mL of CCPs also showed improved stress resistance, a 90.7% increase in glutathione peroxidase (GPX) activity, and a 147.4% increase in glutathione (GSH) content. Transcriptomic analysis showed that CCPs enhanced anti-aging activity by activating the MAPK pathway and inhibiting the IIS pathway, which was associated with protein aggregation. It also reduced lipid synthesis and regulated lipid metabolism through the fat-6 pathway. The results indicated that CCPs could be employed as a valuable ingredient in the food and pharmaceutical fields. |
| format | Article |
| id | doaj-art-84ded8f4edaa42788bbc633477ddf7ca |
| institution | Kabale University |
| issn | 2079-4983 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
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| series | Journal of Functional Biomaterials |
| spelling | doaj-art-84ded8f4edaa42788bbc633477ddf7ca2025-08-20T03:47:57ZengMDPI AGJournal of Functional Biomaterials2079-49832025-04-0116515010.3390/jfb16050150Anti-Aging Effects and Mechanisms of Cod Collagen Peptides (CCPs) in <i>Caenorhabditis elegans</i>Jiale Wei0Junjie Zhang1Nan Ding2Yu Liu3Yuzhen Wu4Rui Duan5Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, 59 Cangwu Road, Lianyungang 222005, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, 59 Cangwu Road, Lianyungang 222005, ChinaCo-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, 59 Cangwu Road, Lianyungang 222005, ChinaSchool of Ocean Food and Biological Engineering, Jiangsu Ocean University, 59 Cangwu Road, Lianyungang 222005, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, 59 Cangwu Road, Lianyungang 222005, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, 59 Cangwu Road, Lianyungang 222005, ChinaGiven the growing interest in natural compounds for promoting healthy aging, this study aimed to investigate the potential of cod collagen peptides (CCPs), a readily available marine resource, to extend lifespan and improve health. Lifespan assays were performed on <i>C. elegans</i> treated with different concentrations of CCPs. Furthermore, various stress resistance assays, including those evaluating oxidative and thermal stress, were conducted. To elucidate the underlying mechanisms, gene expression analysis of key aging-related genes was performed. The results demonstrated that treatment with 25 mg/mL of CCPs extended the lifespan of <i>C. elegans</i> by 13.2%, increased body length and width by 14.8% and 20.6%, respectively, and enhanced head-swing and body-bending frequencies by 66.9% and 80.4%. Lipofuscin content and apoptosis were reduced by 45.9% and 34.1%, respectively. <i>C. elegans</i> treated with 25 mg/mL of CCPs also showed improved stress resistance, a 90.7% increase in glutathione peroxidase (GPX) activity, and a 147.4% increase in glutathione (GSH) content. Transcriptomic analysis showed that CCPs enhanced anti-aging activity by activating the MAPK pathway and inhibiting the IIS pathway, which was associated with protein aggregation. It also reduced lipid synthesis and regulated lipid metabolism through the fat-6 pathway. The results indicated that CCPs could be employed as a valuable ingredient in the food and pharmaceutical fields.https://www.mdpi.com/2079-4983/16/5/150anti-aging<i>Caenorhabditis elegans</i>cod collagen peptidespathway |
| spellingShingle | Jiale Wei Junjie Zhang Nan Ding Yu Liu Yuzhen Wu Rui Duan Anti-Aging Effects and Mechanisms of Cod Collagen Peptides (CCPs) in <i>Caenorhabditis elegans</i> Journal of Functional Biomaterials anti-aging <i>Caenorhabditis elegans</i> cod collagen peptides pathway |
| title | Anti-Aging Effects and Mechanisms of Cod Collagen Peptides (CCPs) in <i>Caenorhabditis elegans</i> |
| title_full | Anti-Aging Effects and Mechanisms of Cod Collagen Peptides (CCPs) in <i>Caenorhabditis elegans</i> |
| title_fullStr | Anti-Aging Effects and Mechanisms of Cod Collagen Peptides (CCPs) in <i>Caenorhabditis elegans</i> |
| title_full_unstemmed | Anti-Aging Effects and Mechanisms of Cod Collagen Peptides (CCPs) in <i>Caenorhabditis elegans</i> |
| title_short | Anti-Aging Effects and Mechanisms of Cod Collagen Peptides (CCPs) in <i>Caenorhabditis elegans</i> |
| title_sort | anti aging effects and mechanisms of cod collagen peptides ccps in i caenorhabditis elegans i |
| topic | anti-aging <i>Caenorhabditis elegans</i> cod collagen peptides pathway |
| url | https://www.mdpi.com/2079-4983/16/5/150 |
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