Bioflocculant production from molasses by Photorhabdus luminescens, flocculation condition optimization and its application in heavy metal removal

A novel biopolymer flocculant was produced from molasses by Photorhabdus luminescens fermentation, and the medium was optimized to increase the yield of biomolecular flocculant by single factor test. Using flocculation rate as the response value, the effect of flocculant on the flocculation effect o...

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Main Author: LIN Jingwen, LIU Zehong, PAN Xieting, LI Mengping, HUANG Shanxin, CHEN Yingqiu, HUANG Dan, YE Yun, LI Xiaolong
Format: Article
Language:English
Published: Editorial Department of China Brewing 2025-01-01
Series:Zhongguo niangzao
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Online Access:https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2025-44-1-247.pdf
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author LIN Jingwen, LIU Zehong, PAN Xieting, LI Mengping, HUANG Shanxin, CHEN Yingqiu, HUANG Dan, YE Yun, LI Xiaolong
author_facet LIN Jingwen, LIU Zehong, PAN Xieting, LI Mengping, HUANG Shanxin, CHEN Yingqiu, HUANG Dan, YE Yun, LI Xiaolong
author_sort LIN Jingwen, LIU Zehong, PAN Xieting, LI Mengping, HUANG Shanxin, CHEN Yingqiu, HUANG Dan, YE Yun, LI Xiaolong
collection DOAJ
description A novel biopolymer flocculant was produced from molasses by Photorhabdus luminescens fermentation, and the medium was optimized to increase the yield of biomolecular flocculant by single factor test. Using flocculation rate as the response value, the effect of flocculant on the flocculation effect of kaolin suspension was studied by single factor test and response surface methodology. The results showed that the optimal medium composition was molasses 5 g/L, pepton 10 g/L, NaCl 5 g/L. Under these conditions, the flocculant yield reached 4.53 g/L. The optimal flocculation conditions were pH 7.0, biological flocculant 200 mg/L, cationic inducer (FeCl<sub>3</sub>) 50 mg/L and stirring rate 240 r/min. Under these conditions, the flocculation rate of flocculant on kaolin reached 93.2%. The removal rates of Cr, Pb, Ni, Cu and Cd were 61.32%, 58.22%, 85.90%, 78.18% and 80.71%, respectively. Molasses was used as carbon source for cheap medium of bioflocculant, which could reduce the culture cost and realize the reuse of waste residue.
format Article
id doaj-art-84d90a3cfac54403b4cf4cf366c58366
institution DOAJ
issn 0254-5071
language English
publishDate 2025-01-01
publisher Editorial Department of China Brewing
record_format Article
series Zhongguo niangzao
spelling doaj-art-84d90a3cfac54403b4cf4cf366c583662025-08-20T02:58:19ZengEditorial Department of China BrewingZhongguo niangzao0254-50712025-01-0144124725210.11882/j.issn.0254-5071.2025.01.036Bioflocculant production from molasses by Photorhabdus luminescens, flocculation condition optimization and its application in heavy metal removalLIN Jingwen, LIU Zehong, PAN Xieting, LI Mengping, HUANG Shanxin, CHEN Yingqiu, HUANG Dan, YE Yun, LI Xiaolong01. College of Biological and Chemical Engineering, Guangxi University of Science and Technology, Liuzhou 545000, China; ;2. School of Food and Bioengineering, Hezhou University, Hezhou 542800, ChinaA novel biopolymer flocculant was produced from molasses by Photorhabdus luminescens fermentation, and the medium was optimized to increase the yield of biomolecular flocculant by single factor test. Using flocculation rate as the response value, the effect of flocculant on the flocculation effect of kaolin suspension was studied by single factor test and response surface methodology. The results showed that the optimal medium composition was molasses 5 g/L, pepton 10 g/L, NaCl 5 g/L. Under these conditions, the flocculant yield reached 4.53 g/L. The optimal flocculation conditions were pH 7.0, biological flocculant 200 mg/L, cationic inducer (FeCl<sub>3</sub>) 50 mg/L and stirring rate 240 r/min. Under these conditions, the flocculation rate of flocculant on kaolin reached 93.2%. The removal rates of Cr, Pb, Ni, Cu and Cd were 61.32%, 58.22%, 85.90%, 78.18% and 80.71%, respectively. Molasses was used as carbon source for cheap medium of bioflocculant, which could reduce the culture cost and realize the reuse of waste residue.https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2025-44-1-247.pdfbioflocculant|<i>photorhabdus luminescens</i>|flocculation condition|response surface methodology|flocculation rate|heavy metal removal
spellingShingle LIN Jingwen, LIU Zehong, PAN Xieting, LI Mengping, HUANG Shanxin, CHEN Yingqiu, HUANG Dan, YE Yun, LI Xiaolong
Bioflocculant production from molasses by Photorhabdus luminescens, flocculation condition optimization and its application in heavy metal removal
Zhongguo niangzao
bioflocculant|<i>photorhabdus luminescens</i>|flocculation condition|response surface methodology|flocculation rate|heavy metal removal
title Bioflocculant production from molasses by Photorhabdus luminescens, flocculation condition optimization and its application in heavy metal removal
title_full Bioflocculant production from molasses by Photorhabdus luminescens, flocculation condition optimization and its application in heavy metal removal
title_fullStr Bioflocculant production from molasses by Photorhabdus luminescens, flocculation condition optimization and its application in heavy metal removal
title_full_unstemmed Bioflocculant production from molasses by Photorhabdus luminescens, flocculation condition optimization and its application in heavy metal removal
title_short Bioflocculant production from molasses by Photorhabdus luminescens, flocculation condition optimization and its application in heavy metal removal
title_sort bioflocculant production from molasses by photorhabdus luminescens flocculation condition optimization and its application in heavy metal removal
topic bioflocculant|<i>photorhabdus luminescens</i>|flocculation condition|response surface methodology|flocculation rate|heavy metal removal
url https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2025-44-1-247.pdf
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