Effect of Alkali Lignin on Laccase Activity and Mycelial Biomass of Flammulina velutipes
The effect of alkali lignin on laccase activity and mycelial biomass of Flammulina velutipes was investigated under different fermentation temperature. The secretion of laccase by F. velutipes has certain specificity. Specifically, laccase activity could not be detected by F. velutipes CCMSSC 00103...
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North Carolina State University
2025-01-01
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| author | Mei-Ling Han Yi-Lin Zhao Tian-Yu Liu Lu-Sen Bian Zi-Han Li Ming-Xue Li Jia Li Xiao-Qi Yang Qi An |
| author_facet | Mei-Ling Han Yi-Lin Zhao Tian-Yu Liu Lu-Sen Bian Zi-Han Li Ming-Xue Li Jia Li Xiao-Qi Yang Qi An |
| author_sort | Mei-Ling Han |
| collection | DOAJ |
| description | The effect of alkali lignin on laccase activity and mycelial biomass of Flammulina velutipes was investigated under different fermentation temperature. The secretion of laccase by F. velutipes has certain specificity. Specifically, laccase activity could not be detected by F. velutipes CCMSSC 00103 under most fermentation medium, but could be detected by F. velutipes FL 19 under more fermentation medium. Among the three strains of F. velutipes, the laccase secretion capacity of F. velutipes FL 19 was obviously better than that of F. velutipes CCMSSC 05331 and F. velutipes CCMSSC 00103. Maximum laccase activity with the value of 73.42 ± 4.74 U/L was secreted by F. velutipes FL 19 in fermentation medium 2 appeared at 2th day. The presence of alkali lignin was useful for improving laccase activity secreted by F. velutipes and the effect of alkali lignin on improving laccase activity exceeded that of Populus beijingensis. F. velutipes were more suitable for secreting laccase at 26 ℃ due to the maximum laccase activity measured at 26 ℃ (73.42 ± 4.74 U/L) higher than that measured at 33 ℃ (70.71 ± 6.89 U/L). The results were useful for selecting suitable medium and temperature of F. velutipes strains to produce low-cost laccase. |
| format | Article |
| id | doaj-art-84c8c3c956ea426e948689989dd5625b |
| institution | Kabale University |
| issn | 1930-2126 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | North Carolina State University |
| record_format | Article |
| series | BioResources |
| spelling | doaj-art-84c8c3c956ea426e948689989dd5625b2025-02-11T00:00:29ZengNorth Carolina State UniversityBioResources1930-21262025-01-01201224322582341Effect of Alkali Lignin on Laccase Activity and Mycelial Biomass of Flammulina velutipesMei-Ling Han0https://orcid.org/0000-0003-4936-3083Yi-Lin Zhao1Tian-Yu Liu2Lu-Sen Bian3https://orcid.org/0000-0003-3660-9056Zi-Han Li4Ming-Xue Li5Jia Li6Xiao-Qi Yang7Qi An8https://orcid.org/0000-0001-5917-2497 College of Life Science, Langfang Normal University, Langfang 065000, Hebei, China; Technical Innovation Center for Utilization of Edible and Medicinal Fungi in Hebei Province, Langfang 065000, Hebei, China; Edible and Medicinal Fungi Research and Development Center of Universities/Colleges in Hebei Province, Langfang 065000, Hebei, ChinaCollege of Life Science, Langfang Normal University, Langfang 065000, Hebei, ChinaCollege of Life Science, Langfang Normal University, Langfang 065000, Hebei, ChinaExperimental Centre of Forestry in North China, Warm Temperate Zone Forestry Jiulong Mountain National Permanent Scientific Research Base, Chinese Academy of Forestry, Beijing 102300, ChinaCollege of Life Science, Langfang Normal University, Langfang 065000, Hebei, ChinaCollege of Life Science, Langfang Normal University, Langfang 065000, Hebei, ChinaCollege of Life Science, Langfang Normal University, Langfang 065000, Hebei, ChinaCollege of Life Science, Langfang Normal University, Langfang 065000, Hebei, ChinaCollege of Life Science, Langfang Normal University, Langfang 065000, Hebei, China; Technical Innovation Center for Utilization of Edible and Medicinal Fungi in Hebei Province, Langfang 065000, Hebei, ChinaThe effect of alkali lignin on laccase activity and mycelial biomass of Flammulina velutipes was investigated under different fermentation temperature. The secretion of laccase by F. velutipes has certain specificity. Specifically, laccase activity could not be detected by F. velutipes CCMSSC 00103 under most fermentation medium, but could be detected by F. velutipes FL 19 under more fermentation medium. Among the three strains of F. velutipes, the laccase secretion capacity of F. velutipes FL 19 was obviously better than that of F. velutipes CCMSSC 05331 and F. velutipes CCMSSC 00103. Maximum laccase activity with the value of 73.42 ± 4.74 U/L was secreted by F. velutipes FL 19 in fermentation medium 2 appeared at 2th day. The presence of alkali lignin was useful for improving laccase activity secreted by F. velutipes and the effect of alkali lignin on improving laccase activity exceeded that of Populus beijingensis. F. velutipes were more suitable for secreting laccase at 26 ℃ due to the maximum laccase activity measured at 26 ℃ (73.42 ± 4.74 U/L) higher than that measured at 33 ℃ (70.71 ± 6.89 U/L). The results were useful for selecting suitable medium and temperature of F. velutipes strains to produce low-cost laccase.https://ojs.bioresources.com/index.php/BRJ/article/view/24104flammulina velutipeslaccasealkali ligninpopulus beijingensismycelial biomass |
| spellingShingle | Mei-Ling Han Yi-Lin Zhao Tian-Yu Liu Lu-Sen Bian Zi-Han Li Ming-Xue Li Jia Li Xiao-Qi Yang Qi An Effect of Alkali Lignin on Laccase Activity and Mycelial Biomass of Flammulina velutipes BioResources flammulina velutipes laccase alkali lignin populus beijingensis mycelial biomass |
| title | Effect of Alkali Lignin on Laccase Activity and Mycelial Biomass of Flammulina velutipes |
| title_full | Effect of Alkali Lignin on Laccase Activity and Mycelial Biomass of Flammulina velutipes |
| title_fullStr | Effect of Alkali Lignin on Laccase Activity and Mycelial Biomass of Flammulina velutipes |
| title_full_unstemmed | Effect of Alkali Lignin on Laccase Activity and Mycelial Biomass of Flammulina velutipes |
| title_short | Effect of Alkali Lignin on Laccase Activity and Mycelial Biomass of Flammulina velutipes |
| title_sort | effect of alkali lignin on laccase activity and mycelial biomass of flammulina velutipes |
| topic | flammulina velutipes laccase alkali lignin populus beijingensis mycelial biomass |
| url | https://ojs.bioresources.com/index.php/BRJ/article/view/24104 |
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