Microbiological activity of bovhyaluronidaze azoximer against microbial biofilms
Objective. To evaluate the ability of bovhyaluronidaze azoximer to destroy biofilms formed by clinical strains of microorganisms. Materials and Methods. The study included 50 clinical strains of Staphylococcus aureus (n = 25), Staphylococcus epidermidis (n = 6), Enterococcus faecalis (n = 8), Es...
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| Format: | Article |
| Language: | Russian |
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Interregional Association for Clinical Microbiology and Antimicrobial Chemotherapy
2024-12-01
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| Series: | Клиническая микробиология и антимикробная химиотерапия |
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| Online Access: | https://cmac-journal.ru/publication/2024/4/cmac-2024-t26-n4-p487/cmac-2024-t26-n4-p487.pdf |
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| author | Kozlov R.S. Tapalskiy D.V. Karpova E.V. Petrovskaya T.A. Kurkova A.A. |
| author_facet | Kozlov R.S. Tapalskiy D.V. Karpova E.V. Petrovskaya T.A. Kurkova A.A. |
| author_sort | Kozlov R.S. |
| collection | DOAJ |
| description | Objective.
To evaluate the ability of bovhyaluronidaze azoximer to destroy biofilms formed by clinical strains of microorganisms.
Materials and Methods.
The study included 50 clinical strains of Staphylococcus aureus (n = 25), Staphylococcus epidermidis (n = 6), Enterococcus faecalis (n = 8), Escherichia coli (n = 9), Candida albicans (n = 2). Antimicrobial susceptibility testing was performed using broth microdilution method. Biofilm formation culturing in presence of antibiotics, or bovhyaluronidaze azoximer, or their combinations was assessed in Mueller-Hinton broth and brain heart broth in 96-well plates. Biofilms were fixed with 2,5% glutaraldehyde solution, stained with 0,25% crystal violet solution, which is extracted by 33% acetic acid solution.
Results.
Bovhyaluronidaze azoximer can destroy the matrix of preformed biofilms of methicillin-resistant strains of S. aureus as well as S. epidermidis, E. coli, C. albicans in concentrations of 64–1000 IU/ml, while exerting a dose-depend effective.
Conclusions.
Bovhyaluronidaze azoximer combining with antibiotics will potentiate its antimicrobial effects by destroying the microbial biofilm matrix formed by microorganisms with multidrug resistance and facilitating the penetration of antibiotics to cellular targets. |
| format | Article |
| id | doaj-art-84be155c3b76440da950c43fbbcdd534 |
| institution | DOAJ |
| issn | 1684-4386 2686-9586 |
| language | Russian |
| publishDate | 2024-12-01 |
| publisher | Interregional Association for Clinical Microbiology and Antimicrobial Chemotherapy |
| record_format | Article |
| series | Клиническая микробиология и антимикробная химиотерапия |
| spelling | doaj-art-84be155c3b76440da950c43fbbcdd5342025-08-20T03:08:00ZrusInterregional Association for Clinical Microbiology and Antimicrobial ChemotherapyКлиническая микробиология и антимикробная химиотерапия1684-43862686-95862024-12-0126448749510.36488/cmac.2024.4.487-495cmac-2024-t26-n4-p487Microbiological activity of bovhyaluronidaze azoximer against microbial biofilmsKozlov R.S.0Tapalskiy D.V.1Karpova E.V.2Petrovskaya T.A.3Kurkova A.A.4Smolensk State Medical University, Smolensk, RussiaInstitute of Physiology of the National Academy of Sciences of Belarus, Minsk, BelarusGomel State Medical University, Gomel, BelarusGomel State Medical University, Gomel, BelarusSmolensk State Medical University, Smolensk, RussiaObjective. To evaluate the ability of bovhyaluronidaze azoximer to destroy biofilms formed by clinical strains of microorganisms. Materials and Methods. The study included 50 clinical strains of Staphylococcus aureus (n = 25), Staphylococcus epidermidis (n = 6), Enterococcus faecalis (n = 8), Escherichia coli (n = 9), Candida albicans (n = 2). Antimicrobial susceptibility testing was performed using broth microdilution method. Biofilm formation culturing in presence of antibiotics, or bovhyaluronidaze azoximer, or their combinations was assessed in Mueller-Hinton broth and brain heart broth in 96-well plates. Biofilms were fixed with 2,5% glutaraldehyde solution, stained with 0,25% crystal violet solution, which is extracted by 33% acetic acid solution. Results. Bovhyaluronidaze azoximer can destroy the matrix of preformed biofilms of methicillin-resistant strains of S. aureus as well as S. epidermidis, E. coli, C. albicans in concentrations of 64–1000 IU/ml, while exerting a dose-depend effective. Conclusions. Bovhyaluronidaze azoximer combining with antibiotics will potentiate its antimicrobial effects by destroying the microbial biofilm matrix formed by microorganisms with multidrug resistance and facilitating the penetration of antibiotics to cellular targets.https://cmac-journal.ru/publication/2024/4/cmac-2024-t26-n4-p487/cmac-2024-t26-n4-p487.pdfbovhyaluronidaze azoximerstaphylococcus aureusstaphylococcus epidermidiscandida albicansenterococcus faecalisescherichia colibiofilm |
| spellingShingle | Kozlov R.S. Tapalskiy D.V. Karpova E.V. Petrovskaya T.A. Kurkova A.A. Microbiological activity of bovhyaluronidaze azoximer against microbial biofilms Клиническая микробиология и антимикробная химиотерапия bovhyaluronidaze azoximer staphylococcus aureus staphylococcus epidermidis candida albicans enterococcus faecalis escherichia coli biofilm |
| title | Microbiological activity of bovhyaluronidaze azoximer against microbial biofilms |
| title_full | Microbiological activity of bovhyaluronidaze azoximer against microbial biofilms |
| title_fullStr | Microbiological activity of bovhyaluronidaze azoximer against microbial biofilms |
| title_full_unstemmed | Microbiological activity of bovhyaluronidaze azoximer against microbial biofilms |
| title_short | Microbiological activity of bovhyaluronidaze azoximer against microbial biofilms |
| title_sort | microbiological activity of bovhyaluronidaze azoximer against microbial biofilms |
| topic | bovhyaluronidaze azoximer staphylococcus aureus staphylococcus epidermidis candida albicans enterococcus faecalis escherichia coli biofilm |
| url | https://cmac-journal.ru/publication/2024/4/cmac-2024-t26-n4-p487/cmac-2024-t26-n4-p487.pdf |
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