CEMP-1 Levels in Periodontal Wound Fluid during the Early Phase of Healing: Prospective Clinical Trial
Objectives. Cementogenesis seems to be significantly compromised during tissue inflammation. In dental practice, surgical procedures are performed with the aim to regenerate periodontium including cementum. However, inflammation that occurs during the initial healing phases after surgery may impair...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2019/1737306 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832558810984611840 |
|---|---|
| author | Claudia Dellavia Elena Canciani Giulio Rasperini Giorgio Pagni Matteo Malvezzi Gaia Pellegrini |
| author_facet | Claudia Dellavia Elena Canciani Giulio Rasperini Giorgio Pagni Matteo Malvezzi Gaia Pellegrini |
| author_sort | Claudia Dellavia |
| collection | DOAJ |
| description | Objectives. Cementogenesis seems to be significantly compromised during tissue inflammation. In dental practice, surgical procedures are performed with the aim to regenerate periodontium including cementum. However, inflammation that occurs during the initial healing phases after surgery may impair regeneration of this tissues. The aim of the present study was to assess if surgical procedures designed to regenerate periodontium might affect levels of cementum protein-1 (CEMP-1) in periodontal wound fluid during early phase of healing. Materials and Methods. In 36 patients, 18 intrabony periodontal defects were treated with regenerative therapy (REG group) and 18 suprabony periodontal defects were treated with open flap debridement (OFD group). In the experimental sites, gingival crevicular fluid was collected immediately before surgery, and periodontal wound fluid was collected 4, 7, 14, and 21 days after surgery. CEMP-1 levels were detected by indirect enzyme-linked immunosorbent assay technique. Results. At the analysis, it resulted that there was a significant average difference in CEMP-1 values between the REG and OFD groups at baseline (p=0.041), the CEMP-1-modeled average in the OFD group was lower by 0.45 ng/ml. There was a significant trend in CEMP-1 over time, and this trend was different among the 2 groups: the REG group showed a statistically significant rising CEMP-1 trend (0.18 ng/ml a week p=0.012), while the OFD had a trend that was significantly lower (-0.22 ng/ml a week compared to the REG group trend p=0.023), the OFD group lost on average 0.05 ng/ml a week. In REG sites, GCF protein levels resulted also related to clinical parameters. Conclusions. During the initial inflammatory phase of periodontal healing, CEMP-1 levels decrease regardless of the surgical protocol applied. The surgical procedures used to regenerate periodontal tissue are able to reverse this trend and to induce significant increase of CEMP-1 in periodontal wound fluid after the first week postop. |
| format | Article |
| id | doaj-art-84b7adaab41f41b5b723bef4f9cc8cae |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-84b7adaab41f41b5b723bef4f9cc8cae2025-02-03T01:31:30ZengWileyMediators of Inflammation0962-93511466-18612019-01-01201910.1155/2019/17373061737306CEMP-1 Levels in Periodontal Wound Fluid during the Early Phase of Healing: Prospective Clinical TrialClaudia Dellavia0Elena Canciani1Giulio Rasperini2Giorgio Pagni3Matteo Malvezzi4Gaia Pellegrini5Department of Biomedical, Surgical and Dental Sciences, Università degli Studi di Milano, Via Mangiagalli 31, 20133 Milan, ItalyDepartment of Biomedical, Surgical and Dental Sciences, Università degli Studi di Milano, Via Mangiagalli 31, 20133 Milan, ItalyDepartment of Biomedical, Surgical and Dental Sciences, Università degli Studi di Milano, Via Mangiagalli 31, 20133 Milan, ItalyDepartment of Biomedical, Surgical and Dental Sciences, Università degli Studi di Milano, Via Mangiagalli 31, 20133 Milan, ItalyDepartment of Clinical Sciences and Community Health, Università degli Studi di Milano, Via Vanzetti 5, 20133 Milan, ItalyDepartment of Biomedical, Surgical and Dental Sciences, Università degli Studi di Milano, Via Mangiagalli 31, 20133 Milan, ItalyObjectives. Cementogenesis seems to be significantly compromised during tissue inflammation. In dental practice, surgical procedures are performed with the aim to regenerate periodontium including cementum. However, inflammation that occurs during the initial healing phases after surgery may impair regeneration of this tissues. The aim of the present study was to assess if surgical procedures designed to regenerate periodontium might affect levels of cementum protein-1 (CEMP-1) in periodontal wound fluid during early phase of healing. Materials and Methods. In 36 patients, 18 intrabony periodontal defects were treated with regenerative therapy (REG group) and 18 suprabony periodontal defects were treated with open flap debridement (OFD group). In the experimental sites, gingival crevicular fluid was collected immediately before surgery, and periodontal wound fluid was collected 4, 7, 14, and 21 days after surgery. CEMP-1 levels were detected by indirect enzyme-linked immunosorbent assay technique. Results. At the analysis, it resulted that there was a significant average difference in CEMP-1 values between the REG and OFD groups at baseline (p=0.041), the CEMP-1-modeled average in the OFD group was lower by 0.45 ng/ml. There was a significant trend in CEMP-1 over time, and this trend was different among the 2 groups: the REG group showed a statistically significant rising CEMP-1 trend (0.18 ng/ml a week p=0.012), while the OFD had a trend that was significantly lower (-0.22 ng/ml a week compared to the REG group trend p=0.023), the OFD group lost on average 0.05 ng/ml a week. In REG sites, GCF protein levels resulted also related to clinical parameters. Conclusions. During the initial inflammatory phase of periodontal healing, CEMP-1 levels decrease regardless of the surgical protocol applied. The surgical procedures used to regenerate periodontal tissue are able to reverse this trend and to induce significant increase of CEMP-1 in periodontal wound fluid after the first week postop.http://dx.doi.org/10.1155/2019/1737306 |
| spellingShingle | Claudia Dellavia Elena Canciani Giulio Rasperini Giorgio Pagni Matteo Malvezzi Gaia Pellegrini CEMP-1 Levels in Periodontal Wound Fluid during the Early Phase of Healing: Prospective Clinical Trial Mediators of Inflammation |
| title | CEMP-1 Levels in Periodontal Wound Fluid during the Early Phase of Healing: Prospective Clinical Trial |
| title_full | CEMP-1 Levels in Periodontal Wound Fluid during the Early Phase of Healing: Prospective Clinical Trial |
| title_fullStr | CEMP-1 Levels in Periodontal Wound Fluid during the Early Phase of Healing: Prospective Clinical Trial |
| title_full_unstemmed | CEMP-1 Levels in Periodontal Wound Fluid during the Early Phase of Healing: Prospective Clinical Trial |
| title_short | CEMP-1 Levels in Periodontal Wound Fluid during the Early Phase of Healing: Prospective Clinical Trial |
| title_sort | cemp 1 levels in periodontal wound fluid during the early phase of healing prospective clinical trial |
| url | http://dx.doi.org/10.1155/2019/1737306 |
| work_keys_str_mv | AT claudiadellavia cemp1levelsinperiodontalwoundfluidduringtheearlyphaseofhealingprospectiveclinicaltrial AT elenacanciani cemp1levelsinperiodontalwoundfluidduringtheearlyphaseofhealingprospectiveclinicaltrial AT giuliorasperini cemp1levelsinperiodontalwoundfluidduringtheearlyphaseofhealingprospectiveclinicaltrial AT giorgiopagni cemp1levelsinperiodontalwoundfluidduringtheearlyphaseofhealingprospectiveclinicaltrial AT matteomalvezzi cemp1levelsinperiodontalwoundfluidduringtheearlyphaseofhealingprospectiveclinicaltrial AT gaiapellegrini cemp1levelsinperiodontalwoundfluidduringtheearlyphaseofhealingprospectiveclinicaltrial |