Prophylactic Effects of Radiofrequency Electromagnetic Field on Pulmonary Ischemia‐Reperfusion via HIF‐1α/eNOS Pathway and BCL2/BAX Signaling
ABSTRACT This study aimed to investigate the vascular effects of a radiofrequency electromagnetic field (RF‐EMF) applied in the lung ischemia and reperfusion (IR) model on the hypoxia‐inducible factor 1 alpha (Hif‐1α)/endothelial nitric oxide synthase (eNOS) pathway and B cell lymphoma 2 (BCL2)/BCL‐...
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| Format: | Article |
| Language: | English |
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Wiley
2025-04-01
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| Series: | Pulmonary Circulation |
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| Online Access: | https://doi.org/10.1002/pul2.70051 |
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| author | Süleyman Emre Akin Halil Asci Muhammet Yusuf Tepebasi İlter Ilhan Özlem Ozmen Selçuk Comlekci Rümeysa Taner Hasan Ekrem Camas Ayşegül Keklik Rasih Yazkan |
| author_facet | Süleyman Emre Akin Halil Asci Muhammet Yusuf Tepebasi İlter Ilhan Özlem Ozmen Selçuk Comlekci Rümeysa Taner Hasan Ekrem Camas Ayşegül Keklik Rasih Yazkan |
| author_sort | Süleyman Emre Akin |
| collection | DOAJ |
| description | ABSTRACT This study aimed to investigate the vascular effects of a radiofrequency electromagnetic field (RF‐EMF) applied in the lung ischemia and reperfusion (IR) model on the hypoxia‐inducible factor 1 alpha (Hif‐1α)/endothelial nitric oxide synthase (eNOS) pathway and B cell lymphoma 2 (BCL2)/BCL‐2 associated X protein (BAX) signaling. Forty male Wistar rats were randomly divided into four groups, each consisting of 10 rats: Sham, IR, IR + RF‐EMF, and RF‐EMF. IR was applied to rats by 60 min of clamping hilus of left lungs and 60 min of reperfusion. Rats were kept in the RF‐EMF unit for 60 min with or without activation. After sacrification, lung tissues were excised for histopathological, immunohistochemical, biochemical, and genetic analyses. IR injury led to increased damage‐related emphysematous findings, significant hyperemia, and increased septal tissue thickness, as observed histopathologically, and immunoexpression levels of tumor necrosis factor‐alpha and caspase‐3. In addition, it was noted that the biochemical parameters total oxidant status, oxidative stress index, and genetic parameters Hif 1 α, eNOS, BAX increased, and BCL2 decreased due to IR damage. In the IR‐RF‐EMF group, improvement has been detected in all parameters. RF‐EMF applied in the IR model exerts antioxidant, antiapoptotic, and anti‐inflammatory effects on lung tissue damage through the Hif‐1α/eNOS pathway and BCL‐2/BAX signaling. The use of RF‐EMF in IR damage is promising, as models that examine the long‐term effects of RF‐EMF at different frequencies. |
| format | Article |
| id | doaj-art-84b027f6873d46e689b6a6bd59d4396d |
| institution | Kabale University |
| issn | 2045-8940 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | Pulmonary Circulation |
| spelling | doaj-art-84b027f6873d46e689b6a6bd59d4396d2025-08-20T03:32:18ZengWileyPulmonary Circulation2045-89402025-04-01152n/an/a10.1002/pul2.70051Prophylactic Effects of Radiofrequency Electromagnetic Field on Pulmonary Ischemia‐Reperfusion via HIF‐1α/eNOS Pathway and BCL2/BAX SignalingSüleyman Emre Akin0Halil Asci1Muhammet Yusuf Tepebasi2İlter Ilhan3Özlem Ozmen4Selçuk Comlekci5Rümeysa Taner6Hasan Ekrem Camas7Ayşegül Keklik8Rasih Yazkan9Department of Thoracic Surgery Suleyman Demirel University Isparta TurkeyDepartment of Medical Pharmacology Suleyman Demirel University Isparta TurkeyDepartment of Medical Genetics Suleyman Demirel University Isparta TurkeyDepartment of Biochemistry Suleyman Demirel University Isparta TurkeyDepartment of Veterinary Pathology Burdur Mehmet Akif Ersoy University Burdur TurkeyDepartment of Bioengineering, Institute of Science Suleyman Demirel University Isparta TurkeyDepartment of Bioengineering, Institute of Science Suleyman Demirel University Isparta TurkeyDepartment of Thoracic Surgery Suleyman Demirel University Isparta TurkeyDepartment of Thoracic Surgery Suleyman Demirel University Isparta TurkeyDepartment of Thoracic Surgery Suleyman Demirel University Isparta TurkeyABSTRACT This study aimed to investigate the vascular effects of a radiofrequency electromagnetic field (RF‐EMF) applied in the lung ischemia and reperfusion (IR) model on the hypoxia‐inducible factor 1 alpha (Hif‐1α)/endothelial nitric oxide synthase (eNOS) pathway and B cell lymphoma 2 (BCL2)/BCL‐2 associated X protein (BAX) signaling. Forty male Wistar rats were randomly divided into four groups, each consisting of 10 rats: Sham, IR, IR + RF‐EMF, and RF‐EMF. IR was applied to rats by 60 min of clamping hilus of left lungs and 60 min of reperfusion. Rats were kept in the RF‐EMF unit for 60 min with or without activation. After sacrification, lung tissues were excised for histopathological, immunohistochemical, biochemical, and genetic analyses. IR injury led to increased damage‐related emphysematous findings, significant hyperemia, and increased septal tissue thickness, as observed histopathologically, and immunoexpression levels of tumor necrosis factor‐alpha and caspase‐3. In addition, it was noted that the biochemical parameters total oxidant status, oxidative stress index, and genetic parameters Hif 1 α, eNOS, BAX increased, and BCL2 decreased due to IR damage. In the IR‐RF‐EMF group, improvement has been detected in all parameters. RF‐EMF applied in the IR model exerts antioxidant, antiapoptotic, and anti‐inflammatory effects on lung tissue damage through the Hif‐1α/eNOS pathway and BCL‐2/BAX signaling. The use of RF‐EMF in IR damage is promising, as models that examine the long‐term effects of RF‐EMF at different frequencies.https://doi.org/10.1002/pul2.70051apoptosisinflammationischemia‐reperfusion injurylungRF‐EMF |
| spellingShingle | Süleyman Emre Akin Halil Asci Muhammet Yusuf Tepebasi İlter Ilhan Özlem Ozmen Selçuk Comlekci Rümeysa Taner Hasan Ekrem Camas Ayşegül Keklik Rasih Yazkan Prophylactic Effects of Radiofrequency Electromagnetic Field on Pulmonary Ischemia‐Reperfusion via HIF‐1α/eNOS Pathway and BCL2/BAX Signaling Pulmonary Circulation apoptosis inflammation ischemia‐reperfusion injury lung RF‐EMF |
| title | Prophylactic Effects of Radiofrequency Electromagnetic Field on Pulmonary Ischemia‐Reperfusion via HIF‐1α/eNOS Pathway and BCL2/BAX Signaling |
| title_full | Prophylactic Effects of Radiofrequency Electromagnetic Field on Pulmonary Ischemia‐Reperfusion via HIF‐1α/eNOS Pathway and BCL2/BAX Signaling |
| title_fullStr | Prophylactic Effects of Radiofrequency Electromagnetic Field on Pulmonary Ischemia‐Reperfusion via HIF‐1α/eNOS Pathway and BCL2/BAX Signaling |
| title_full_unstemmed | Prophylactic Effects of Radiofrequency Electromagnetic Field on Pulmonary Ischemia‐Reperfusion via HIF‐1α/eNOS Pathway and BCL2/BAX Signaling |
| title_short | Prophylactic Effects of Radiofrequency Electromagnetic Field on Pulmonary Ischemia‐Reperfusion via HIF‐1α/eNOS Pathway and BCL2/BAX Signaling |
| title_sort | prophylactic effects of radiofrequency electromagnetic field on pulmonary ischemia reperfusion via hif 1α enos pathway and bcl2 bax signaling |
| topic | apoptosis inflammation ischemia‐reperfusion injury lung RF‐EMF |
| url | https://doi.org/10.1002/pul2.70051 |
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