Prophylactic Effects of Radiofrequency Electromagnetic Field on Pulmonary Ischemia‐Reperfusion via HIF‐1α/eNOS Pathway and BCL2/BAX Signaling
ABSTRACT This study aimed to investigate the vascular effects of a radiofrequency electromagnetic field (RF‐EMF) applied in the lung ischemia and reperfusion (IR) model on the hypoxia‐inducible factor 1 alpha (Hif‐1α)/endothelial nitric oxide synthase (eNOS) pathway and B cell lymphoma 2 (BCL2)/BCL‐...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2025-04-01
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| Series: | Pulmonary Circulation |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/pul2.70051 |
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| Summary: | ABSTRACT This study aimed to investigate the vascular effects of a radiofrequency electromagnetic field (RF‐EMF) applied in the lung ischemia and reperfusion (IR) model on the hypoxia‐inducible factor 1 alpha (Hif‐1α)/endothelial nitric oxide synthase (eNOS) pathway and B cell lymphoma 2 (BCL2)/BCL‐2 associated X protein (BAX) signaling. Forty male Wistar rats were randomly divided into four groups, each consisting of 10 rats: Sham, IR, IR + RF‐EMF, and RF‐EMF. IR was applied to rats by 60 min of clamping hilus of left lungs and 60 min of reperfusion. Rats were kept in the RF‐EMF unit for 60 min with or without activation. After sacrification, lung tissues were excised for histopathological, immunohistochemical, biochemical, and genetic analyses. IR injury led to increased damage‐related emphysematous findings, significant hyperemia, and increased septal tissue thickness, as observed histopathologically, and immunoexpression levels of tumor necrosis factor‐alpha and caspase‐3. In addition, it was noted that the biochemical parameters total oxidant status, oxidative stress index, and genetic parameters Hif 1 α, eNOS, BAX increased, and BCL2 decreased due to IR damage. In the IR‐RF‐EMF group, improvement has been detected in all parameters. RF‐EMF applied in the IR model exerts antioxidant, antiapoptotic, and anti‐inflammatory effects on lung tissue damage through the Hif‐1α/eNOS pathway and BCL‐2/BAX signaling. The use of RF‐EMF in IR damage is promising, as models that examine the long‐term effects of RF‐EMF at different frequencies. |
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| ISSN: | 2045-8940 |