The association of single nucleotide polymorphisms in tumor necrosis factor 3 with susceptibility/resistance of Magallana gigas to Halomonas sp. 7T
Tumor necrosis factors (TNFs) are a group of multifunctional inflammatory cytokines involved in various pathological and immune processes. In the present study, a TNF-3 was characterized in Pacific oyster Magallana gigas (MgTNF-3), and its association of single nucleotide polymorphisms with suscepti...
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Elsevier
2025-06-01
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| Series: | Comparative Immunology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S295031162500014X |
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| author | Hongmei Fan Qingyu Peng Tianbao Liu Lei Fang Chuanyan Yang Ning Kong Lingling Wang Linsheng Song |
| author_facet | Hongmei Fan Qingyu Peng Tianbao Liu Lei Fang Chuanyan Yang Ning Kong Lingling Wang Linsheng Song |
| author_sort | Hongmei Fan |
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| description | Tumor necrosis factors (TNFs) are a group of multifunctional inflammatory cytokines involved in various pathological and immune processes. In the present study, a TNF-3 was characterized in Pacific oyster Magallana gigas (MgTNF-3), and its association of single nucleotide polymorphisms with susceptibility/resistance of oyster to Halomonas sp. 7T were investigated. MgTNF-3 was constituted of a typical TNF domain and a transmembrane region, and shared low sequence identity and similarity with the TNF superfamily members from vertebrates and other invertebrates. The MgTNF-3 mRNA was constitutively expressed in haemocytes and all the examined tissues with the highest expression level in adductor muscle, and was significantly induced by lipopolysaccharide stimulation. The gene coding region sequence of MgTNF-3 gene from two oyster populations (the bacterial-resistant population and the common population) was cloned, and the polymorphisms within this region were investigated to analyze their association with bacterial resistance. A total of 17 single nucleotide polymorphisms (SNPs) were identified, and seven ones including +17103 G/A, +17111 A/G, +17132 T/A, +17145 C/T, +17169 A/G, +17175 A/G and +17337 C/T were found to be associated with bacterial resistance after Hardy-Weinberg equilibrium (HWE) and association analysis. Moreover, haplotype AGAGT was associated with bacterial resistance based on the result of linkage disequilibrium and association analysis. These results provided insights into the molecular mechanism of different oyster populations responding to bacteria, which suggested that the increased bacterial resistance of bacterial-resistant population (compared with common population) was associated with the higher expression of MgTNF-3. Meanwhile, the seven genotypes (+17103 G/G, +17111 A/A, +17132 A/T, +17145 C/T, +17169 A/A, +17175 G/G and +17337 T/T) and one haplotype (AGAGT) could be used as potential markers for oyster selection breeding with higher bacterial resistance. |
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| id | doaj-art-84aa774af7b047a89967849a0c28b4cd |
| institution | Kabale University |
| issn | 2950-3116 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
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| series | Comparative Immunology Reports |
| spelling | doaj-art-84aa774af7b047a89967849a0c28b4cd2025-02-12T05:33:18ZengElsevierComparative Immunology Reports2950-31162025-06-018200208The association of single nucleotide polymorphisms in tumor necrosis factor 3 with susceptibility/resistance of Magallana gigas to Halomonas sp. 7THongmei Fan0Qingyu Peng1Tianbao Liu2Lei Fang3Chuanyan Yang4Ning Kong5Lingling Wang6Linsheng Song7Liaoning Key Laboratory of Marine Animal Immunology & Disease Control, Dalian Ocean University, Dalian 116023, PR China; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, PR ChinaLiaoning Key Laboratory of Marine Animal Immunology & Disease Control, Dalian Ocean University, Dalian 116023, PR China; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, PR ChinaLiaoning Key Laboratory of Marine Animal Immunology & Disease Control, Dalian Ocean University, Dalian 116023, PR China; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, PR ChinaCollege of Marine Science and Technology and Environment, Dalian Ocean University, Dalian 116023, PR ChinaLiaoning Key Laboratory of Marine Animal Immunology & Disease Control, Dalian Ocean University, Dalian 116023, PR China; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, PR China; College of Marine Science and Technology and Environment, Dalian Ocean University, Dalian 116023, PR China; Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian 116023, PR China; Corresponding author at: Dalian Ocean University, 52 Heishijiao Street, Dalian 116023, PR China.Liaoning Key Laboratory of Marine Animal Immunology & Disease Control, Dalian Ocean University, Dalian 116023, PR China; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, PR China; College of Marine Science and Technology and Environment, Dalian Ocean University, Dalian 116023, PR China; Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian 116023, PR ChinaLiaoning Key Laboratory of Marine Animal Immunology & Disease Control, Dalian Ocean University, Dalian 116023, PR China; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, PR China; College of Marine Science and Technology and Environment, Dalian Ocean University, Dalian 116023, PR China; Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian 116023, PR China; Corresponding author at: Dalian Ocean University, 52 Heishijiao Street, Dalian 116023, PR China.Liaoning Key Laboratory of Marine Animal Immunology & Disease Control, Dalian Ocean University, Dalian 116023, PR China; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, PR China; College of Marine Science and Technology and Environment, Dalian Ocean University, Dalian 116023, PR China; Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian 116023, PR ChinaTumor necrosis factors (TNFs) are a group of multifunctional inflammatory cytokines involved in various pathological and immune processes. In the present study, a TNF-3 was characterized in Pacific oyster Magallana gigas (MgTNF-3), and its association of single nucleotide polymorphisms with susceptibility/resistance of oyster to Halomonas sp. 7T were investigated. MgTNF-3 was constituted of a typical TNF domain and a transmembrane region, and shared low sequence identity and similarity with the TNF superfamily members from vertebrates and other invertebrates. The MgTNF-3 mRNA was constitutively expressed in haemocytes and all the examined tissues with the highest expression level in adductor muscle, and was significantly induced by lipopolysaccharide stimulation. The gene coding region sequence of MgTNF-3 gene from two oyster populations (the bacterial-resistant population and the common population) was cloned, and the polymorphisms within this region were investigated to analyze their association with bacterial resistance. A total of 17 single nucleotide polymorphisms (SNPs) were identified, and seven ones including +17103 G/A, +17111 A/G, +17132 T/A, +17145 C/T, +17169 A/G, +17175 A/G and +17337 C/T were found to be associated with bacterial resistance after Hardy-Weinberg equilibrium (HWE) and association analysis. Moreover, haplotype AGAGT was associated with bacterial resistance based on the result of linkage disequilibrium and association analysis. These results provided insights into the molecular mechanism of different oyster populations responding to bacteria, which suggested that the increased bacterial resistance of bacterial-resistant population (compared with common population) was associated with the higher expression of MgTNF-3. Meanwhile, the seven genotypes (+17103 G/G, +17111 A/A, +17132 A/T, +17145 C/T, +17169 A/A, +17175 G/G and +17337 T/T) and one haplotype (AGAGT) could be used as potential markers for oyster selection breeding with higher bacterial resistance.http://www.sciencedirect.com/science/article/pii/S295031162500014XMagallana gigasTumor necrosis factorGene codingSingle nucleotide polymorphismResistance to Halomonas sp. 7T |
| spellingShingle | Hongmei Fan Qingyu Peng Tianbao Liu Lei Fang Chuanyan Yang Ning Kong Lingling Wang Linsheng Song The association of single nucleotide polymorphisms in tumor necrosis factor 3 with susceptibility/resistance of Magallana gigas to Halomonas sp. 7T Comparative Immunology Reports Magallana gigas Tumor necrosis factor Gene coding Single nucleotide polymorphism Resistance to Halomonas sp. 7T |
| title | The association of single nucleotide polymorphisms in tumor necrosis factor 3 with susceptibility/resistance of Magallana gigas to Halomonas sp. 7T |
| title_full | The association of single nucleotide polymorphisms in tumor necrosis factor 3 with susceptibility/resistance of Magallana gigas to Halomonas sp. 7T |
| title_fullStr | The association of single nucleotide polymorphisms in tumor necrosis factor 3 with susceptibility/resistance of Magallana gigas to Halomonas sp. 7T |
| title_full_unstemmed | The association of single nucleotide polymorphisms in tumor necrosis factor 3 with susceptibility/resistance of Magallana gigas to Halomonas sp. 7T |
| title_short | The association of single nucleotide polymorphisms in tumor necrosis factor 3 with susceptibility/resistance of Magallana gigas to Halomonas sp. 7T |
| title_sort | association of single nucleotide polymorphisms in tumor necrosis factor 3 with susceptibility resistance of magallana gigas to halomonas sp 7t |
| topic | Magallana gigas Tumor necrosis factor Gene coding Single nucleotide polymorphism Resistance to Halomonas sp. 7T |
| url | http://www.sciencedirect.com/science/article/pii/S295031162500014X |
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