The association of single nucleotide polymorphisms in tumor necrosis factor 3 with susceptibility/resistance of Magallana gigas to Halomonas sp. 7T
Tumor necrosis factors (TNFs) are a group of multifunctional inflammatory cytokines involved in various pathological and immune processes. In the present study, a TNF-3 was characterized in Pacific oyster Magallana gigas (MgTNF-3), and its association of single nucleotide polymorphisms with suscepti...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-06-01
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| Series: | Comparative Immunology Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S295031162500014X |
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| Summary: | Tumor necrosis factors (TNFs) are a group of multifunctional inflammatory cytokines involved in various pathological and immune processes. In the present study, a TNF-3 was characterized in Pacific oyster Magallana gigas (MgTNF-3), and its association of single nucleotide polymorphisms with susceptibility/resistance of oyster to Halomonas sp. 7T were investigated. MgTNF-3 was constituted of a typical TNF domain and a transmembrane region, and shared low sequence identity and similarity with the TNF superfamily members from vertebrates and other invertebrates. The MgTNF-3 mRNA was constitutively expressed in haemocytes and all the examined tissues with the highest expression level in adductor muscle, and was significantly induced by lipopolysaccharide stimulation. The gene coding region sequence of MgTNF-3 gene from two oyster populations (the bacterial-resistant population and the common population) was cloned, and the polymorphisms within this region were investigated to analyze their association with bacterial resistance. A total of 17 single nucleotide polymorphisms (SNPs) were identified, and seven ones including +17103 G/A, +17111 A/G, +17132 T/A, +17145 C/T, +17169 A/G, +17175 A/G and +17337 C/T were found to be associated with bacterial resistance after Hardy-Weinberg equilibrium (HWE) and association analysis. Moreover, haplotype AGAGT was associated with bacterial resistance based on the result of linkage disequilibrium and association analysis. These results provided insights into the molecular mechanism of different oyster populations responding to bacteria, which suggested that the increased bacterial resistance of bacterial-resistant population (compared with common population) was associated with the higher expression of MgTNF-3. Meanwhile, the seven genotypes (+17103 G/G, +17111 A/A, +17132 A/T, +17145 C/T, +17169 A/A, +17175 G/G and +17337 T/T) and one haplotype (AGAGT) could be used as potential markers for oyster selection breeding with higher bacterial resistance. |
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| ISSN: | 2950-3116 |