Development and Validation of a High-Performance Liquid Chromatographic Method for the Determination of Cinitapride in Human Plasma
A precise and reliable reversed-phase high-performance liquid chromatographic method with ultraviolet detection was developed and validated to determine cinitapride in human plasma. After liquid-liquid extraction, chromatographic separation was achieved on a Nucleosil C18 (25 cm × 4.6 mm, 5 µm) colu...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2018/8280762 |
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| _version_ | 1832548684468846592 |
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| author | Boovizhikannan Thangabalan Getu Kahsay Tadele Eticha |
| author_facet | Boovizhikannan Thangabalan Getu Kahsay Tadele Eticha |
| author_sort | Boovizhikannan Thangabalan |
| collection | DOAJ |
| description | A precise and reliable reversed-phase high-performance liquid chromatographic method with ultraviolet detection was developed and validated to determine cinitapride in human plasma. After liquid-liquid extraction, chromatographic separation was achieved on a Nucleosil C18 (25 cm × 4.6 mm, 5 µm) column with an isocratic elution consisting of 10 mM ammonium acetate (pH 5.2), methanol, and acetonitrile, 40 : 50 : 10, v/v/v. The developed method was validated as per US FDA guidelines for its linearity, selectivity, sensitivity, precision, accuracy, and stability. Satisfactory findings were obtained from the validation studies. The linearity range of the method was 1 to 35 ng/mL while the extraction recovery of cinitapride in human plasma was more than 86%. The percent coefficient of variation of both intraday and interday precision was ≤7.1%. |
| format | Article |
| id | doaj-art-849fcdcdb75845e2af7b57ff52f5d273 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-849fcdcdb75845e2af7b57ff52f5d2732025-02-03T06:13:19ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732018-01-01201810.1155/2018/82807628280762Development and Validation of a High-Performance Liquid Chromatographic Method for the Determination of Cinitapride in Human PlasmaBoovizhikannan Thangabalan0Getu Kahsay1Tadele Eticha2School of Pharmacy, College of Health Sciences, Mekelle University, Mekelle, EthiopiaSchool of Pharmacy, College of Health Sciences, Mekelle University, Mekelle, EthiopiaSchool of Pharmacy, College of Health Sciences, Mekelle University, Mekelle, EthiopiaA precise and reliable reversed-phase high-performance liquid chromatographic method with ultraviolet detection was developed and validated to determine cinitapride in human plasma. After liquid-liquid extraction, chromatographic separation was achieved on a Nucleosil C18 (25 cm × 4.6 mm, 5 µm) column with an isocratic elution consisting of 10 mM ammonium acetate (pH 5.2), methanol, and acetonitrile, 40 : 50 : 10, v/v/v. The developed method was validated as per US FDA guidelines for its linearity, selectivity, sensitivity, precision, accuracy, and stability. Satisfactory findings were obtained from the validation studies. The linearity range of the method was 1 to 35 ng/mL while the extraction recovery of cinitapride in human plasma was more than 86%. The percent coefficient of variation of both intraday and interday precision was ≤7.1%.http://dx.doi.org/10.1155/2018/8280762 |
| spellingShingle | Boovizhikannan Thangabalan Getu Kahsay Tadele Eticha Development and Validation of a High-Performance Liquid Chromatographic Method for the Determination of Cinitapride in Human Plasma Journal of Analytical Methods in Chemistry |
| title | Development and Validation of a High-Performance Liquid Chromatographic Method for the Determination of Cinitapride in Human Plasma |
| title_full | Development and Validation of a High-Performance Liquid Chromatographic Method for the Determination of Cinitapride in Human Plasma |
| title_fullStr | Development and Validation of a High-Performance Liquid Chromatographic Method for the Determination of Cinitapride in Human Plasma |
| title_full_unstemmed | Development and Validation of a High-Performance Liquid Chromatographic Method for the Determination of Cinitapride in Human Plasma |
| title_short | Development and Validation of a High-Performance Liquid Chromatographic Method for the Determination of Cinitapride in Human Plasma |
| title_sort | development and validation of a high performance liquid chromatographic method for the determination of cinitapride in human plasma |
| url | http://dx.doi.org/10.1155/2018/8280762 |
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