Structural Identification of <i>Physalis alkekengi</i> L. Polysaccharides
<i>Physalis alkekengi</i> L. fruit polysaccharides can reduce blood sugar, regulate blood lipids, and improve intestinal flora structure. However, the specific polysaccharide components exerting these effects are unclear. In this study, we extracted, separated, purified, and characterize...
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2025-02-01
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| author | Yun Zhang Xuan Wen Neng Xu Hongyan Fu Ge Lv Wenjie Yu Lina Wei Lin Zhao |
| author_facet | Yun Zhang Xuan Wen Neng Xu Hongyan Fu Ge Lv Wenjie Yu Lina Wei Lin Zhao |
| author_sort | Yun Zhang |
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| description | <i>Physalis alkekengi</i> L. fruit polysaccharides can reduce blood sugar, regulate blood lipids, and improve intestinal flora structure. However, the specific polysaccharide components exerting these effects are unclear. In this study, we extracted, separated, purified, and characterized the <i>P. alkekengi</i> polysaccharides Phy-1a, Phy-1b, and Phy-1c. Ion chromatography showed that Phy-1b was mainly composed of rhamnose, arabinose, galactose, glucose, and xylose at a molar ratio of 3.0:19.8:47.5:20.9:8.8, and Phy-1c was composed of rhamnose, arabinose, galactose, glucose, xylose, mannose, ribose Galactosamine hydrochloride and Glucosamine hydrochloride at a molar ratio of 10.4:7.9:22.8:30.5:4.6:4.4:19.4:3.9:5.8. Neither of these polysaccharides contained uronic acid, indicating their neutral property. Methylation analysis and nuclear magnetic resonance spectroscopy showed that Phy-1b was mainly composed of terminal sugars (1-Araf); 1,5-Araf; 1,4-Xylp; 1-Glcp; 2,4-Rhap; 1,3-Glcp; 1,4-Galp; 1,4-Glcp; 1,3-Galp; 1,6-Glcp; 1,3,6-Glcp; and 1,4,6-Galp at a molar ratio of 5.2:7.1:7.8:13.7:6.3:11.2:7.0:16.3:7.4:6.0:6.8:5.3, with the main chain being →2)-α-L-Rhap-(1→4)-β-<span style="font-variant: small-caps;">d</span>-Galp-(1→4)-β-<span style="font-variant: small-caps;">d</span>-Galp-(1→[3)-β-<span style="font-variant: small-caps;">d</span>-Glcp-(1]2→3)-β-<span style="font-variant: small-caps;">d</span>-Glcp-(1→[4)-β-<span style="font-variant: small-caps;">d</span>-Glcp-(1]2→ and the branched chains being β-L-Araf-(1→5)-β-L-Araf-(1→, β-d-Glcp-(1→4)-β-d-Xylp-(1→ 3)-β-d-Galp-(1→, and β-d-Glcp-(1→6)-β-d-Glcp-(1→. The three fragments, respectively, pass through the O-4 key of →2,4)-α-l-Rhap-(1→, O-6 key of →4,6)-β-d-Galp-(1→, and O-6 of →3,6)-β-d-Glcp-(1→ connected to the main chain. These results provide a reference for enhancing the utilization value of <i>P. alkekengi</i> resources to promote its high-value and efficient processing. |
| format | Article |
| id | doaj-art-849f71ce9c874a78982e057748834f65 |
| institution | DOAJ |
| issn | 1420-3049 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
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| series | Molecules |
| spelling | doaj-art-849f71ce9c874a78982e057748834f652025-08-20T03:12:22ZengMDPI AGMolecules1420-30492025-02-0130494910.3390/molecules30040949Structural Identification of <i>Physalis alkekengi</i> L. PolysaccharidesYun Zhang0Xuan Wen1Neng Xu2Hongyan Fu3Ge Lv4Wenjie Yu5Lina Wei6Lin Zhao7College of Food Engineering, Heilongjiang East University, Harbin 150066, ChinaCollege of Food Engineering, Heilongjiang East University, Harbin 150066, ChinaCollege of Food Engineering, Heilongjiang East University, Harbin 150066, ChinaCollege of Food Engineering, Heilongjiang East University, Harbin 150066, ChinaCollege of Food Engineering, Heilongjiang East University, Harbin 150066, ChinaCollege of Food Engineering, Heilongjiang East University, Harbin 150066, ChinaCollege of Food Engineering, Heilongjiang East University, Harbin 150066, ChinaQuality & Safety Institute of Agricultural Products, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China<i>Physalis alkekengi</i> L. fruit polysaccharides can reduce blood sugar, regulate blood lipids, and improve intestinal flora structure. However, the specific polysaccharide components exerting these effects are unclear. In this study, we extracted, separated, purified, and characterized the <i>P. alkekengi</i> polysaccharides Phy-1a, Phy-1b, and Phy-1c. Ion chromatography showed that Phy-1b was mainly composed of rhamnose, arabinose, galactose, glucose, and xylose at a molar ratio of 3.0:19.8:47.5:20.9:8.8, and Phy-1c was composed of rhamnose, arabinose, galactose, glucose, xylose, mannose, ribose Galactosamine hydrochloride and Glucosamine hydrochloride at a molar ratio of 10.4:7.9:22.8:30.5:4.6:4.4:19.4:3.9:5.8. Neither of these polysaccharides contained uronic acid, indicating their neutral property. Methylation analysis and nuclear magnetic resonance spectroscopy showed that Phy-1b was mainly composed of terminal sugars (1-Araf); 1,5-Araf; 1,4-Xylp; 1-Glcp; 2,4-Rhap; 1,3-Glcp; 1,4-Galp; 1,4-Glcp; 1,3-Galp; 1,6-Glcp; 1,3,6-Glcp; and 1,4,6-Galp at a molar ratio of 5.2:7.1:7.8:13.7:6.3:11.2:7.0:16.3:7.4:6.0:6.8:5.3, with the main chain being →2)-α-L-Rhap-(1→4)-β-<span style="font-variant: small-caps;">d</span>-Galp-(1→4)-β-<span style="font-variant: small-caps;">d</span>-Galp-(1→[3)-β-<span style="font-variant: small-caps;">d</span>-Glcp-(1]2→3)-β-<span style="font-variant: small-caps;">d</span>-Glcp-(1→[4)-β-<span style="font-variant: small-caps;">d</span>-Glcp-(1]2→ and the branched chains being β-L-Araf-(1→5)-β-L-Araf-(1→, β-d-Glcp-(1→4)-β-d-Xylp-(1→ 3)-β-d-Galp-(1→, and β-d-Glcp-(1→6)-β-d-Glcp-(1→. The three fragments, respectively, pass through the O-4 key of →2,4)-α-l-Rhap-(1→, O-6 key of →4,6)-β-d-Galp-(1→, and O-6 of →3,6)-β-d-Glcp-(1→ connected to the main chain. These results provide a reference for enhancing the utilization value of <i>P. alkekengi</i> resources to promote its high-value and efficient processing.https://www.mdpi.com/1420-3049/30/4/949<i>Physalis alkekengi</i> L.polysaccharideion chromatographymethylationnuclear magnetic resonance spectrum analysisstructural identification |
| spellingShingle | Yun Zhang Xuan Wen Neng Xu Hongyan Fu Ge Lv Wenjie Yu Lina Wei Lin Zhao Structural Identification of <i>Physalis alkekengi</i> L. Polysaccharides Molecules <i>Physalis alkekengi</i> L. polysaccharide ion chromatography methylation nuclear magnetic resonance spectrum analysis structural identification |
| title | Structural Identification of <i>Physalis alkekengi</i> L. Polysaccharides |
| title_full | Structural Identification of <i>Physalis alkekengi</i> L. Polysaccharides |
| title_fullStr | Structural Identification of <i>Physalis alkekengi</i> L. Polysaccharides |
| title_full_unstemmed | Structural Identification of <i>Physalis alkekengi</i> L. Polysaccharides |
| title_short | Structural Identification of <i>Physalis alkekengi</i> L. Polysaccharides |
| title_sort | structural identification of i physalis alkekengi i l polysaccharides |
| topic | <i>Physalis alkekengi</i> L. polysaccharide ion chromatography methylation nuclear magnetic resonance spectrum analysis structural identification |
| url | https://www.mdpi.com/1420-3049/30/4/949 |
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