Endoplasmic reticulum stress-dependent regulation of the expression of serine hydroxymethyltransferase 2 in glioblastoma cells
Objective. Serine hydroxymethyltransferase (SHMT2) plays a multifunctional role in mitochondria (folate-dependent tRNA methylation, translation, and thymidylate synthesis). The endoplasmic reticulum stress, hypoxia, and glucose and glutamine supply are significant factors of malignant tumor growth i...
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| Format: | Article |
| Language: | English |
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Sciendo
2024-01-01
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| Series: | Endocrine Regulations |
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| Online Access: | https://doi.org/10.2478/enr-2024-0016 |
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| author | Minchenko Oleksandr H. Sliusar Myroslava Y. Khita Olena O. Viletska Yuliia M. Luzina Olha Y. Danilovskyi Serhiy V. Minchenko Dmytro O. |
| author_facet | Minchenko Oleksandr H. Sliusar Myroslava Y. Khita Olena O. Viletska Yuliia M. Luzina Olha Y. Danilovskyi Serhiy V. Minchenko Dmytro O. |
| author_sort | Minchenko Oleksandr H. |
| collection | DOAJ |
| description | Objective. Serine hydroxymethyltransferase (SHMT2) plays a multifunctional role in mitochondria (folate-dependent tRNA methylation, translation, and thymidylate synthesis). The endoplasmic reticulum stress, hypoxia, and glucose and glutamine supply are significant factors of malignant tumor growth including glioblastoma. Previous studies have shown that the knockdown of the endoplasmic reticulum to nucleus signaling 1 (ERN1) pathway of endoplasmic reticulum stress strongly suppressed glioblastoma cell proliferation and modified the sensitivity of these cells to hypoxia and glucose or glutamine deprivations. The present study aimed to investigate the regulation of the SHMT2 gene in U87MG glioblastoma cells by ERN1 knockdown, hypoxia, and glucose or glutamine deprivations with the intent to reveal the role of ERN1 signaling in sensitivity of this gene expression to hypoxia and nutrient supply. |
| format | Article |
| id | doaj-art-8492f2f39bcd4c8f97216ef7a540237f |
| institution | OA Journals |
| issn | 1336-0329 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Endocrine Regulations |
| spelling | doaj-art-8492f2f39bcd4c8f97216ef7a540237f2025-08-20T01:53:33ZengSciendoEndocrine Regulations1336-03292024-01-0158114415210.2478/enr-2024-0016Endoplasmic reticulum stress-dependent regulation of the expression of serine hydroxymethyltransferase 2 in glioblastoma cellsMinchenko Oleksandr H.0Sliusar Myroslava Y.1Khita Olena O.2Viletska Yuliia M.3Luzina Olha Y.4Danilovskyi Serhiy V.5Minchenko Dmytro O.6Department of Molecular Biology, Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv, UkraineDepartment of Molecular Biology, Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv, UkraineDepartment of Molecular Biology, Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv, UkraineDepartment of Molecular Biology, Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv, UkraineDepartment of Molecular Biology, Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv, UkraineDepartment of Molecular Biology, Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv, UkraineDepartment of Molecular Biology, Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv, UkraineObjective. Serine hydroxymethyltransferase (SHMT2) plays a multifunctional role in mitochondria (folate-dependent tRNA methylation, translation, and thymidylate synthesis). The endoplasmic reticulum stress, hypoxia, and glucose and glutamine supply are significant factors of malignant tumor growth including glioblastoma. Previous studies have shown that the knockdown of the endoplasmic reticulum to nucleus signaling 1 (ERN1) pathway of endoplasmic reticulum stress strongly suppressed glioblastoma cell proliferation and modified the sensitivity of these cells to hypoxia and glucose or glutamine deprivations. The present study aimed to investigate the regulation of the SHMT2 gene in U87MG glioblastoma cells by ERN1 knockdown, hypoxia, and glucose or glutamine deprivations with the intent to reveal the role of ERN1 signaling in sensitivity of this gene expression to hypoxia and nutrient supply.https://doi.org/10.2478/enr-2024-0016ern1 knockdownhypoxianutrient deprivationshmt2gene expressionu87mg cells |
| spellingShingle | Minchenko Oleksandr H. Sliusar Myroslava Y. Khita Olena O. Viletska Yuliia M. Luzina Olha Y. Danilovskyi Serhiy V. Minchenko Dmytro O. Endoplasmic reticulum stress-dependent regulation of the expression of serine hydroxymethyltransferase 2 in glioblastoma cells Endocrine Regulations ern1 knockdown hypoxia nutrient deprivation shmt2 gene expression u87mg cells |
| title | Endoplasmic reticulum stress-dependent regulation of the expression of serine hydroxymethyltransferase 2 in glioblastoma cells |
| title_full | Endoplasmic reticulum stress-dependent regulation of the expression of serine hydroxymethyltransferase 2 in glioblastoma cells |
| title_fullStr | Endoplasmic reticulum stress-dependent regulation of the expression of serine hydroxymethyltransferase 2 in glioblastoma cells |
| title_full_unstemmed | Endoplasmic reticulum stress-dependent regulation of the expression of serine hydroxymethyltransferase 2 in glioblastoma cells |
| title_short | Endoplasmic reticulum stress-dependent regulation of the expression of serine hydroxymethyltransferase 2 in glioblastoma cells |
| title_sort | endoplasmic reticulum stress dependent regulation of the expression of serine hydroxymethyltransferase 2 in glioblastoma cells |
| topic | ern1 knockdown hypoxia nutrient deprivation shmt2 gene expression u87mg cells |
| url | https://doi.org/10.2478/enr-2024-0016 |
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