Effect of hydroalcoholic extract of Tanacetum parthenium on gene expression and paraoxonase 1 enzyme activity: An experimental and molecular dynamic study
Background and aims: Cardiovascular diseases are among the most common causes of death worldwide. The paraoxonase 1 (PON1) enzyme is a main factor in preventing these diseases. This study investigated the effect of hydroalcoholic extract of Tanacetum parthenium plant on the activity and gene express...
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Shahrekord University of Medical Sciences
2024-09-01
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| Series: | Journal of Shahrekord University of Medical Sciences |
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| Online Access: | https://j.skums.ac.ir/PDF/jskums-26-88.pdf |
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| author | Javad Saffari-Chaleshtori Fatemeh Maghsoudi Hojatollah Rohi-Brojeni Keyhan Ghatreh-Samani |
| author_facet | Javad Saffari-Chaleshtori Fatemeh Maghsoudi Hojatollah Rohi-Brojeni Keyhan Ghatreh-Samani |
| author_sort | Javad Saffari-Chaleshtori |
| collection | DOAJ |
| description | Background and aims: Cardiovascular diseases are among the most common causes of death worldwide. The paraoxonase 1 (PON1) enzyme is a main factor in preventing these diseases. This study investigated the effect of hydroalcoholic extract of Tanacetum parthenium plant on the activity and gene expression of PON1. Methods: Forty male rats were divided into 4 groups: a control group, a hyperlipidemic group, and two hyperlipidemic groups that were treated with 400 and 800 mg/kg of hydroalcoholic extract. Arylesterase activity of PON1 and serum triglyceride and cholesterol levels were measured. Moreover, the gene expression of PON1 in the liver tissue samples was measured using real-time PCR. Finally, molecular dynamics studies of apigenin as a main compound in the plant were performed to investigate the activity of PON1 in the simulated environment. Results: Hydroalcoholic extract of T. parthenium decreased serum triglyceride (from 105.8±10.1 to 60.5±21.5 and 50.9±11.2 mg/dL) and total cholesterol (from 97.5±16.8 to 59.6±8.5 and 52.0±9.6 mg/dL) levels, while it increased the activity of arylesterase paraoxonase1 significantly (P<0.001). It also showed a significant (P<0.001) effect on PON1 gene expression. However, the dose of 800 mg/kg was more effective. The results of simulation and molecular dynamics showed that apigenin binds to PON1 with a high affinity and induces changes in the molecular dynamics parameters. Conclusion: The 800 mg/kg of hydroalcoholic extract of T. parthenium can increase the activity of PON1 and its gene expression. It seems that apigenin as one of the most important antioxidant compounds of this plant can increase the activity of this enzyme by direct binding to PON1. |
| format | Article |
| id | doaj-art-847b8e005a554b7ba50909402213e2a4 |
| institution | OA Journals |
| issn | 2717-0071 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Shahrekord University of Medical Sciences |
| record_format | Article |
| series | Journal of Shahrekord University of Medical Sciences |
| spelling | doaj-art-847b8e005a554b7ba50909402213e2a42025-08-20T02:08:16ZengShahrekord University of Medical SciencesJournal of Shahrekord University of Medical Sciences2717-00712024-09-01263889310.34172/jsums.782jskums-782Effect of hydroalcoholic extract of Tanacetum parthenium on gene expression and paraoxonase 1 enzyme activity: An experimental and molecular dynamic studyJavad Saffari-Chaleshtori0Fatemeh Maghsoudi1Hojatollah Rohi-Brojeni2Keyhan Ghatreh-Samani3Clinical Biochemistry Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, IranMedical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, IranClinical Biochemistry Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, IranClinical Biochemistry Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, IranBackground and aims: Cardiovascular diseases are among the most common causes of death worldwide. The paraoxonase 1 (PON1) enzyme is a main factor in preventing these diseases. This study investigated the effect of hydroalcoholic extract of Tanacetum parthenium plant on the activity and gene expression of PON1. Methods: Forty male rats were divided into 4 groups: a control group, a hyperlipidemic group, and two hyperlipidemic groups that were treated with 400 and 800 mg/kg of hydroalcoholic extract. Arylesterase activity of PON1 and serum triglyceride and cholesterol levels were measured. Moreover, the gene expression of PON1 in the liver tissue samples was measured using real-time PCR. Finally, molecular dynamics studies of apigenin as a main compound in the plant were performed to investigate the activity of PON1 in the simulated environment. Results: Hydroalcoholic extract of T. parthenium decreased serum triglyceride (from 105.8±10.1 to 60.5±21.5 and 50.9±11.2 mg/dL) and total cholesterol (from 97.5±16.8 to 59.6±8.5 and 52.0±9.6 mg/dL) levels, while it increased the activity of arylesterase paraoxonase1 significantly (P<0.001). It also showed a significant (P<0.001) effect on PON1 gene expression. However, the dose of 800 mg/kg was more effective. The results of simulation and molecular dynamics showed that apigenin binds to PON1 with a high affinity and induces changes in the molecular dynamics parameters. Conclusion: The 800 mg/kg of hydroalcoholic extract of T. parthenium can increase the activity of PON1 and its gene expression. It seems that apigenin as one of the most important antioxidant compounds of this plant can increase the activity of this enzyme by direct binding to PON1.https://j.skums.ac.ir/PDF/jskums-26-88.pdfparaoxonase1tanacetum partheniumapigenindockingmolecular dynamics |
| spellingShingle | Javad Saffari-Chaleshtori Fatemeh Maghsoudi Hojatollah Rohi-Brojeni Keyhan Ghatreh-Samani Effect of hydroalcoholic extract of Tanacetum parthenium on gene expression and paraoxonase 1 enzyme activity: An experimental and molecular dynamic study Journal of Shahrekord University of Medical Sciences paraoxonase1 tanacetum parthenium apigenin docking molecular dynamics |
| title | Effect of hydroalcoholic extract of Tanacetum parthenium on gene expression and paraoxonase 1 enzyme activity: An experimental and molecular dynamic study |
| title_full | Effect of hydroalcoholic extract of Tanacetum parthenium on gene expression and paraoxonase 1 enzyme activity: An experimental and molecular dynamic study |
| title_fullStr | Effect of hydroalcoholic extract of Tanacetum parthenium on gene expression and paraoxonase 1 enzyme activity: An experimental and molecular dynamic study |
| title_full_unstemmed | Effect of hydroalcoholic extract of Tanacetum parthenium on gene expression and paraoxonase 1 enzyme activity: An experimental and molecular dynamic study |
| title_short | Effect of hydroalcoholic extract of Tanacetum parthenium on gene expression and paraoxonase 1 enzyme activity: An experimental and molecular dynamic study |
| title_sort | effect of hydroalcoholic extract of tanacetum parthenium on gene expression and paraoxonase 1 enzyme activity an experimental and molecular dynamic study |
| topic | paraoxonase1 tanacetum parthenium apigenin docking molecular dynamics |
| url | https://j.skums.ac.ir/PDF/jskums-26-88.pdf |
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