Spectroscopic and Docking Studies on the Binding of Liquiritigenin with Hyaluronidase for Antiallergic Mechanism
The inhibitory effect of liquiritigenin on hyaluronidase and its binding mechanism were investigated systematically by UV-vis absorption, fluorescence, and molecular modeling approaches. These results indicated that liquiritigenin could interact with hyaluronidase to form a liquiritigenin-hyaluronid...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Scientifica |
| Online Access: | http://dx.doi.org/10.1155/2016/9178097 |
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| _version_ | 1849401769159819264 |
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| author | Hua-jin Zeng Ran Yang Jing You Ling-bo Qu Yan-jun Sun |
| author_facet | Hua-jin Zeng Ran Yang Jing You Ling-bo Qu Yan-jun Sun |
| author_sort | Hua-jin Zeng |
| collection | DOAJ |
| description | The inhibitory effect of liquiritigenin on hyaluronidase and its binding mechanism were investigated systematically by UV-vis absorption, fluorescence, and molecular modeling approaches. These results indicated that liquiritigenin could interact with hyaluronidase to form a liquiritigenin-hyaluronidase complex. The binding constant, number of binding sites, and thermodynamic parameters were calculated, which indicated that liquiritigenin could spontaneously bind with hyaluronidase mainly through electrostatic and hydrophobic interactions with one binding site. Synchronous fluorescence, three-dimensional fluorescence, and molecular docking results revealed that liquiritigenin bound directly to the enzyme cavity site and this binding influenced the microenvironment of the hyaluronidase activity site, resulting in reduced hyaluronidase activity. The present study provides useful information for clinical applications of liquiritigenin as a hyaluronidase inhibitor. |
| format | Article |
| id | doaj-art-8477dcf234794f7d80063b1dae09fe36 |
| institution | Kabale University |
| issn | 2090-908X |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Scientifica |
| spelling | doaj-art-8477dcf234794f7d80063b1dae09fe362025-08-20T03:37:42ZengWileyScientifica2090-908X2016-01-01201610.1155/2016/91780979178097Spectroscopic and Docking Studies on the Binding of Liquiritigenin with Hyaluronidase for Antiallergic MechanismHua-jin Zeng0Ran Yang1Jing You2Ling-bo Qu3Yan-jun Sun4School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001, ChinaCollege of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, ChinaSchool of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001, ChinaCollege of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, ChinaCollege of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, ChinaThe inhibitory effect of liquiritigenin on hyaluronidase and its binding mechanism were investigated systematically by UV-vis absorption, fluorescence, and molecular modeling approaches. These results indicated that liquiritigenin could interact with hyaluronidase to form a liquiritigenin-hyaluronidase complex. The binding constant, number of binding sites, and thermodynamic parameters were calculated, which indicated that liquiritigenin could spontaneously bind with hyaluronidase mainly through electrostatic and hydrophobic interactions with one binding site. Synchronous fluorescence, three-dimensional fluorescence, and molecular docking results revealed that liquiritigenin bound directly to the enzyme cavity site and this binding influenced the microenvironment of the hyaluronidase activity site, resulting in reduced hyaluronidase activity. The present study provides useful information for clinical applications of liquiritigenin as a hyaluronidase inhibitor.http://dx.doi.org/10.1155/2016/9178097 |
| spellingShingle | Hua-jin Zeng Ran Yang Jing You Ling-bo Qu Yan-jun Sun Spectroscopic and Docking Studies on the Binding of Liquiritigenin with Hyaluronidase for Antiallergic Mechanism Scientifica |
| title | Spectroscopic and Docking Studies on the Binding of Liquiritigenin with Hyaluronidase for Antiallergic Mechanism |
| title_full | Spectroscopic and Docking Studies on the Binding of Liquiritigenin with Hyaluronidase for Antiallergic Mechanism |
| title_fullStr | Spectroscopic and Docking Studies on the Binding of Liquiritigenin with Hyaluronidase for Antiallergic Mechanism |
| title_full_unstemmed | Spectroscopic and Docking Studies on the Binding of Liquiritigenin with Hyaluronidase for Antiallergic Mechanism |
| title_short | Spectroscopic and Docking Studies on the Binding of Liquiritigenin with Hyaluronidase for Antiallergic Mechanism |
| title_sort | spectroscopic and docking studies on the binding of liquiritigenin with hyaluronidase for antiallergic mechanism |
| url | http://dx.doi.org/10.1155/2016/9178097 |
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