Lung Mesenchymal Stem Cells Ameliorate Elastase-Induced Damage in an Animal Model of Emphysema
Pulmonary emphysema is a respiratory condition characterized by alveolar destruction that leads to airflow limitation and reduced lung function. Although with extensive research, the pathophysiology of emphysema is poorly understood and effective treatments are still missing. Evidence suggests that...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2018/9492038 |
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| author | Donato Cappetta Antonella De Angelis Giuseppe Spaziano Gioia Tartaglione Elena Piegari Grazia Esposito Loreta Pia Ciuffreda Angela Liparulo Manuela Sgambato Teresa Palmira Russo Francesco Rossi Liberato Berrino Konrad Urbanek Bruno D’Agostino |
| author_facet | Donato Cappetta Antonella De Angelis Giuseppe Spaziano Gioia Tartaglione Elena Piegari Grazia Esposito Loreta Pia Ciuffreda Angela Liparulo Manuela Sgambato Teresa Palmira Russo Francesco Rossi Liberato Berrino Konrad Urbanek Bruno D’Agostino |
| author_sort | Donato Cappetta |
| collection | DOAJ |
| description | Pulmonary emphysema is a respiratory condition characterized by alveolar destruction that leads to airflow limitation and reduced lung function. Although with extensive research, the pathophysiology of emphysema is poorly understood and effective treatments are still missing. Evidence suggests that mesenchymal stem cells (MSCs) possess the ability to engraft the injured tissues and induce repair via a paracrine effect. Thus, the aim of this study was to test the effects of the intratracheal administration of lung-derived mouse MSCs in a model of elastase-induced emphysema. Pulmonary function (static lung compliance) showed an increased stiffness induced by elastase, while morphometric findings (mean linear intercept and tissue/alveolar area) confirmed the severity of alveolar disruption. Contrarily, MSC administration partially restored lung elasticity and alveolar architecture. In the absence of evidence that MSCs acquired epithelial phenotype, we detected an increased proliferative activity of aquaporin 5- and surfactant protein C-positive lung cells, suggesting MSC-driven paracrine mechanisms. The data indicate the mediation of hepatocyte growth factor in amplifying MSC-driven tissue response after injury. Our study shed light on supportive properties of lung-derived MSCs, although the full identification of mechanisms orchestrated by MSCs and responsible for epithelial repair after injury is a critical aspect yet to be achieved. |
| format | Article |
| id | doaj-art-84731956cbdd48de9b2cfd2ec1576f8f |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-84731956cbdd48de9b2cfd2ec1576f8f2025-08-20T03:37:42ZengWileyStem Cells International1687-966X1687-96782018-01-01201810.1155/2018/94920389492038Lung Mesenchymal Stem Cells Ameliorate Elastase-Induced Damage in an Animal Model of EmphysemaDonato Cappetta0Antonella De Angelis1Giuseppe Spaziano2Gioia Tartaglione3Elena Piegari4Grazia Esposito5Loreta Pia Ciuffreda6Angela Liparulo7Manuela Sgambato8Teresa Palmira Russo9Francesco Rossi10Liberato Berrino11Konrad Urbanek12Bruno D’Agostino13Department of Experimental Medicine, Section of Pharmacology, University of Campania “Luigi Vanvitelli”, Naples, ItalyDepartment of Experimental Medicine, Section of Pharmacology, University of Campania “Luigi Vanvitelli”, Naples, ItalyDepartment of Experimental Medicine, Section of Pharmacology, University of Campania “Luigi Vanvitelli”, Naples, ItalyDepartment of Experimental Medicine, Section of Pharmacology, University of Campania “Luigi Vanvitelli”, Naples, ItalyDepartment of Experimental Medicine, Section of Pharmacology, University of Campania “Luigi Vanvitelli”, Naples, ItalyDepartment of Experimental Medicine, Section of Pharmacology, University of Campania “Luigi Vanvitelli”, Naples, ItalyDepartment of Experimental Medicine, Section of Pharmacology, University of Campania “Luigi Vanvitelli”, Naples, ItalyDepartment of Experimental Medicine, Section of Pharmacology, University of Campania “Luigi Vanvitelli”, Naples, ItalyDepartment of Experimental Medicine, Section of Pharmacology, University of Campania “Luigi Vanvitelli”, Naples, ItalyDepartment of Experimental Medicine, Section of Pharmacology, University of Campania “Luigi Vanvitelli”, Naples, ItalyDepartment of Experimental Medicine, Section of Pharmacology, University of Campania “Luigi Vanvitelli”, Naples, ItalyDepartment of Experimental Medicine, Section of Pharmacology, University of Campania “Luigi Vanvitelli”, Naples, ItalyDepartment of Experimental Medicine, Section of Pharmacology, University of Campania “Luigi Vanvitelli”, Naples, ItalyDepartment of Experimental Medicine, Section of Pharmacology, University of Campania “Luigi Vanvitelli”, Naples, ItalyPulmonary emphysema is a respiratory condition characterized by alveolar destruction that leads to airflow limitation and reduced lung function. Although with extensive research, the pathophysiology of emphysema is poorly understood and effective treatments are still missing. Evidence suggests that mesenchymal stem cells (MSCs) possess the ability to engraft the injured tissues and induce repair via a paracrine effect. Thus, the aim of this study was to test the effects of the intratracheal administration of lung-derived mouse MSCs in a model of elastase-induced emphysema. Pulmonary function (static lung compliance) showed an increased stiffness induced by elastase, while morphometric findings (mean linear intercept and tissue/alveolar area) confirmed the severity of alveolar disruption. Contrarily, MSC administration partially restored lung elasticity and alveolar architecture. In the absence of evidence that MSCs acquired epithelial phenotype, we detected an increased proliferative activity of aquaporin 5- and surfactant protein C-positive lung cells, suggesting MSC-driven paracrine mechanisms. The data indicate the mediation of hepatocyte growth factor in amplifying MSC-driven tissue response after injury. Our study shed light on supportive properties of lung-derived MSCs, although the full identification of mechanisms orchestrated by MSCs and responsible for epithelial repair after injury is a critical aspect yet to be achieved.http://dx.doi.org/10.1155/2018/9492038 |
| spellingShingle | Donato Cappetta Antonella De Angelis Giuseppe Spaziano Gioia Tartaglione Elena Piegari Grazia Esposito Loreta Pia Ciuffreda Angela Liparulo Manuela Sgambato Teresa Palmira Russo Francesco Rossi Liberato Berrino Konrad Urbanek Bruno D’Agostino Lung Mesenchymal Stem Cells Ameliorate Elastase-Induced Damage in an Animal Model of Emphysema Stem Cells International |
| title | Lung Mesenchymal Stem Cells Ameliorate Elastase-Induced Damage in an Animal Model of Emphysema |
| title_full | Lung Mesenchymal Stem Cells Ameliorate Elastase-Induced Damage in an Animal Model of Emphysema |
| title_fullStr | Lung Mesenchymal Stem Cells Ameliorate Elastase-Induced Damage in an Animal Model of Emphysema |
| title_full_unstemmed | Lung Mesenchymal Stem Cells Ameliorate Elastase-Induced Damage in an Animal Model of Emphysema |
| title_short | Lung Mesenchymal Stem Cells Ameliorate Elastase-Induced Damage in an Animal Model of Emphysema |
| title_sort | lung mesenchymal stem cells ameliorate elastase induced damage in an animal model of emphysema |
| url | http://dx.doi.org/10.1155/2018/9492038 |
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