A neonatal rat model of progressive left ventricular pressure overload induced by abdominal aortic banding microsurgeryCentral MessagePerspective
Objective: Left ventricular pressure overload models using adult mice or rats were developed 60 years ago; however, a neonatal mouse model of left ventricular pressure overload was reported only 5 years ago. Moreover, how left ventricular pressure overload reshapes the neonatal left ventricle and ho...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-08-01
|
| Series: | JTCVS Techniques |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666250725001610 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849239470719631360 |
|---|---|
| author | Zheng Wang, MD Sixie Zheng, MD Lincai Ye, MD, PhD Debao Li, MD He Zhang, MD Yingying Xiao, MD, PhD Chenxi Liu, MD Yuqing Hu, MD Sijuan Sun, MD Peisen Ruan, MD Hao Chen, MD, PhD Qi Sun, MD, PhD |
| author_facet | Zheng Wang, MD Sixie Zheng, MD Lincai Ye, MD, PhD Debao Li, MD He Zhang, MD Yingying Xiao, MD, PhD Chenxi Liu, MD Yuqing Hu, MD Sijuan Sun, MD Peisen Ruan, MD Hao Chen, MD, PhD Qi Sun, MD, PhD |
| author_sort | Zheng Wang, MD |
| collection | DOAJ |
| description | Objective: Left ventricular pressure overload models using adult mice or rats were developed 60 years ago; however, a neonatal mouse model of left ventricular pressure overload was reported only 5 years ago. Moreover, how left ventricular pressure overload reshapes the neonatal left ventricle and how it affects cardiomyocyte proliferation remain largely unexplored. The aim of this study is to develop a simple neonatal rat model with clinical features matched to those of left ventricular pressure overload. Methods: A neonatal rat model of progressive left ventricular pressure overload was created via abdominal aortic banding microsurgery at postnatal day 1 and verified by gross examination at postnatal day 7, abdominal ultrasound at postnatal day 21, and left upper limb blood pressure measurement from postoperative day 21 to day 35. A surgical video and detailed surgical procedures were documented for learning purposes. Results: RNA sequencing demonstrated that there were only 171 differentially expressed genes between the abdominal aortic banding surgery and sham left ventricles at postnatal day 3, with 406 differentially expressed genes at postnatal day 7. At postnatal day 3, there was little enrichment of proliferation-associated genes and only a small percentage of proliferating cardiomyocytes; at postnatal day 7, there was an abundant enrichment of proliferation-associated genes and a large percentage of proliferating cardiomyocytes, exactly opposite to the neonatal transverse aortic constriction surgery model, which exhibited decreased cardiomyocyte proliferation over time and even inhibited cardiomyocyte proliferation when severe left ventricular pressure overload was induced by transverse aortic constriction surgery. Moreover, abdominal aortic banding surgery does not require a thoracotomy, resulting in a success rate as high as 100%. Conclusions: A neonatal rat model of progressive left ventricular pressure overload was successfully established and fully documented to provide a platform for pediatric left ventricular pressure overload–associated investigation. |
| format | Article |
| id | doaj-art-8467e976a8e943ada804f0c789d7cedf |
| institution | Kabale University |
| issn | 2666-2507 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | JTCVS Techniques |
| spelling | doaj-art-8467e976a8e943ada804f0c789d7cedf2025-08-20T04:01:00ZengElsevierJTCVS Techniques2666-25072025-08-013211913510.1016/j.xjtc.2025.04.014A neonatal rat model of progressive left ventricular pressure overload induced by abdominal aortic banding microsurgeryCentral MessagePerspectiveZheng Wang, MD0Sixie Zheng, MD1Lincai Ye, MD, PhD2Debao Li, MD3He Zhang, MD4Yingying Xiao, MD, PhD5Chenxi Liu, MD6Yuqing Hu, MD7Sijuan Sun, MD8Peisen Ruan, MD9Hao Chen, MD, PhD10Qi Sun, MD, PhD11Department of Thoracic and Cardiovascular Surgery, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Thoracic and Cardiovascular Surgery, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Thoracic and Cardiovascular Surgery, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Shanghai Institute For Pediatric Congenital Heart Disease, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Pediatric Surgery, Children's Hospital of Fudan University, National Children's Medical Center, Shanghai, ChinaDepartment of Critical Care Medicine, Women and Children's Hospital of Ningbo University, Ningbo, Zhejiang, ChinaDepartment of Thoracic and Cardiovascular Surgery, Shanghai Children's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Rheumatology and Immunology, Shanghai Children's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Cardiology, Shanghai Children's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Pediatric Intensive Care Unit, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Critical Care Medicine, Women and Children's Hospital of Ningbo University, Ningbo, Zhejiang, China; Address for reprints: Peisen Ruan, MD, Department of Critical Care Medicine, The Affiliated Women and Children's Hospital of Ningbo University, No. 339 Liuting St, Haishu District, Ningbo, Zhejiang Province, China.Department of Thoracic and Cardiovascular Surgery, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Hao Chen, MD, and Qi Sun, MD, PhD, Department of Thoracic and Cardiovascular Surgery, Shanghai Children’s Medical Center, Shanghai Jiao Tong University School of Medicine, 1678 Dongfang Rd, Shanghai 200127, China.Department of Thoracic and Cardiovascular Surgery, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Shanghai Institute For Pediatric Congenital Heart Disease, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Hao Chen, MD, and Qi Sun, MD, PhD, Department of Thoracic and Cardiovascular Surgery, Shanghai Children’s Medical Center, Shanghai Jiao Tong University School of Medicine, 1678 Dongfang Rd, Shanghai 200127, China.Objective: Left ventricular pressure overload models using adult mice or rats were developed 60 years ago; however, a neonatal mouse model of left ventricular pressure overload was reported only 5 years ago. Moreover, how left ventricular pressure overload reshapes the neonatal left ventricle and how it affects cardiomyocyte proliferation remain largely unexplored. The aim of this study is to develop a simple neonatal rat model with clinical features matched to those of left ventricular pressure overload. Methods: A neonatal rat model of progressive left ventricular pressure overload was created via abdominal aortic banding microsurgery at postnatal day 1 and verified by gross examination at postnatal day 7, abdominal ultrasound at postnatal day 21, and left upper limb blood pressure measurement from postoperative day 21 to day 35. A surgical video and detailed surgical procedures were documented for learning purposes. Results: RNA sequencing demonstrated that there were only 171 differentially expressed genes between the abdominal aortic banding surgery and sham left ventricles at postnatal day 3, with 406 differentially expressed genes at postnatal day 7. At postnatal day 3, there was little enrichment of proliferation-associated genes and only a small percentage of proliferating cardiomyocytes; at postnatal day 7, there was an abundant enrichment of proliferation-associated genes and a large percentage of proliferating cardiomyocytes, exactly opposite to the neonatal transverse aortic constriction surgery model, which exhibited decreased cardiomyocyte proliferation over time and even inhibited cardiomyocyte proliferation when severe left ventricular pressure overload was induced by transverse aortic constriction surgery. Moreover, abdominal aortic banding surgery does not require a thoracotomy, resulting in a success rate as high as 100%. Conclusions: A neonatal rat model of progressive left ventricular pressure overload was successfully established and fully documented to provide a platform for pediatric left ventricular pressure overload–associated investigation.http://www.sciencedirect.com/science/article/pii/S2666250725001610cardiomyocyteleft ventricular pressure overloadpediatricsproliferation |
| spellingShingle | Zheng Wang, MD Sixie Zheng, MD Lincai Ye, MD, PhD Debao Li, MD He Zhang, MD Yingying Xiao, MD, PhD Chenxi Liu, MD Yuqing Hu, MD Sijuan Sun, MD Peisen Ruan, MD Hao Chen, MD, PhD Qi Sun, MD, PhD A neonatal rat model of progressive left ventricular pressure overload induced by abdominal aortic banding microsurgeryCentral MessagePerspective JTCVS Techniques cardiomyocyte left ventricular pressure overload pediatrics proliferation |
| title | A neonatal rat model of progressive left ventricular pressure overload induced by abdominal aortic banding microsurgeryCentral MessagePerspective |
| title_full | A neonatal rat model of progressive left ventricular pressure overload induced by abdominal aortic banding microsurgeryCentral MessagePerspective |
| title_fullStr | A neonatal rat model of progressive left ventricular pressure overload induced by abdominal aortic banding microsurgeryCentral MessagePerspective |
| title_full_unstemmed | A neonatal rat model of progressive left ventricular pressure overload induced by abdominal aortic banding microsurgeryCentral MessagePerspective |
| title_short | A neonatal rat model of progressive left ventricular pressure overload induced by abdominal aortic banding microsurgeryCentral MessagePerspective |
| title_sort | neonatal rat model of progressive left ventricular pressure overload induced by abdominal aortic banding microsurgerycentral messageperspective |
| topic | cardiomyocyte left ventricular pressure overload pediatrics proliferation |
| url | http://www.sciencedirect.com/science/article/pii/S2666250725001610 |
| work_keys_str_mv | AT zhengwangmd aneonatalratmodelofprogressiveleftventricularpressureoverloadinducedbyabdominalaorticbandingmicrosurgerycentralmessageperspective AT sixiezhengmd aneonatalratmodelofprogressiveleftventricularpressureoverloadinducedbyabdominalaorticbandingmicrosurgerycentralmessageperspective AT lincaiyemdphd aneonatalratmodelofprogressiveleftventricularpressureoverloadinducedbyabdominalaorticbandingmicrosurgerycentralmessageperspective AT debaolimd aneonatalratmodelofprogressiveleftventricularpressureoverloadinducedbyabdominalaorticbandingmicrosurgerycentralmessageperspective AT hezhangmd aneonatalratmodelofprogressiveleftventricularpressureoverloadinducedbyabdominalaorticbandingmicrosurgerycentralmessageperspective AT yingyingxiaomdphd aneonatalratmodelofprogressiveleftventricularpressureoverloadinducedbyabdominalaorticbandingmicrosurgerycentralmessageperspective AT chenxiliumd aneonatalratmodelofprogressiveleftventricularpressureoverloadinducedbyabdominalaorticbandingmicrosurgerycentralmessageperspective AT yuqinghumd aneonatalratmodelofprogressiveleftventricularpressureoverloadinducedbyabdominalaorticbandingmicrosurgerycentralmessageperspective AT sijuansunmd aneonatalratmodelofprogressiveleftventricularpressureoverloadinducedbyabdominalaorticbandingmicrosurgerycentralmessageperspective AT peisenruanmd aneonatalratmodelofprogressiveleftventricularpressureoverloadinducedbyabdominalaorticbandingmicrosurgerycentralmessageperspective AT haochenmdphd aneonatalratmodelofprogressiveleftventricularpressureoverloadinducedbyabdominalaorticbandingmicrosurgerycentralmessageperspective AT qisunmdphd aneonatalratmodelofprogressiveleftventricularpressureoverloadinducedbyabdominalaorticbandingmicrosurgerycentralmessageperspective AT zhengwangmd neonatalratmodelofprogressiveleftventricularpressureoverloadinducedbyabdominalaorticbandingmicrosurgerycentralmessageperspective AT sixiezhengmd neonatalratmodelofprogressiveleftventricularpressureoverloadinducedbyabdominalaorticbandingmicrosurgerycentralmessageperspective AT lincaiyemdphd neonatalratmodelofprogressiveleftventricularpressureoverloadinducedbyabdominalaorticbandingmicrosurgerycentralmessageperspective AT debaolimd neonatalratmodelofprogressiveleftventricularpressureoverloadinducedbyabdominalaorticbandingmicrosurgerycentralmessageperspective AT hezhangmd neonatalratmodelofprogressiveleftventricularpressureoverloadinducedbyabdominalaorticbandingmicrosurgerycentralmessageperspective AT yingyingxiaomdphd neonatalratmodelofprogressiveleftventricularpressureoverloadinducedbyabdominalaorticbandingmicrosurgerycentralmessageperspective AT chenxiliumd neonatalratmodelofprogressiveleftventricularpressureoverloadinducedbyabdominalaorticbandingmicrosurgerycentralmessageperspective AT yuqinghumd neonatalratmodelofprogressiveleftventricularpressureoverloadinducedbyabdominalaorticbandingmicrosurgerycentralmessageperspective AT sijuansunmd neonatalratmodelofprogressiveleftventricularpressureoverloadinducedbyabdominalaorticbandingmicrosurgerycentralmessageperspective AT peisenruanmd neonatalratmodelofprogressiveleftventricularpressureoverloadinducedbyabdominalaorticbandingmicrosurgerycentralmessageperspective AT haochenmdphd neonatalratmodelofprogressiveleftventricularpressureoverloadinducedbyabdominalaorticbandingmicrosurgerycentralmessageperspective AT qisunmdphd neonatalratmodelofprogressiveleftventricularpressureoverloadinducedbyabdominalaorticbandingmicrosurgerycentralmessageperspective |