Alternative-NHEJ is a mechanistically distinct pathway of mammalian chromosome break repair.
Characterizing the functional overlap and mutagenic potential of different pathways of chromosomal double-strand break (DSB) repair is important to understand how mutations arise during cancer development and treatment. To this end, we have compared the role of individual factors in three different...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2008-06-01
|
| Series: | PLoS Genetics |
| Online Access: | https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1000110&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850240509935616000 |
|---|---|
| author | Nicole Bennardo Anita Cheng Nick Huang Jeremy M Stark |
| author_facet | Nicole Bennardo Anita Cheng Nick Huang Jeremy M Stark |
| author_sort | Nicole Bennardo |
| collection | DOAJ |
| description | Characterizing the functional overlap and mutagenic potential of different pathways of chromosomal double-strand break (DSB) repair is important to understand how mutations arise during cancer development and treatment. To this end, we have compared the role of individual factors in three different pathways of mammalian DSB repair: alternative-nonhomologous end joining (alt-NHEJ), single-strand annealing (SSA), and homology directed repair (HDR/GC). Considering early steps of repair, we found that the DSB end-processing factors KU and CtIP affect all three pathways similarly, in that repair is suppressed by KU and promoted by CtIP. In contrast, both KU and CtIP appear dispensable for the absolute level of total-NHEJ between two tandem I-SceI-induced DSBs. During later steps of repair, we find that while the annealing and processing factors RAD52 and ERCC1 are important to promote SSA, both HDR/GC and alt-NHEJ are significantly less dependent upon these factors. As well, while disruption of RAD51 causes a decrease in HDR/GC and an increase in SSA, inhibition of this factor did not affect alt-NHEJ. These results suggest that the regulation of DSB end-processing via KU/CtIP is a common step during alt-NHEJ, SSA, and HDR/GC. However, at later steps of repair, alt-NHEJ is a mechanistically distinct pathway of DSB repair, and thus may play a unique role in mutagenesis during cancer development and therapy. |
| format | Article |
| id | doaj-art-8447b4238eff4c5a85124cbdf27fc4c5 |
| institution | OA Journals |
| issn | 1553-7390 1553-7404 |
| language | English |
| publishDate | 2008-06-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj-art-8447b4238eff4c5a85124cbdf27fc4c52025-08-20T02:00:51ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042008-06-0146e100011010.1371/journal.pgen.1000110Alternative-NHEJ is a mechanistically distinct pathway of mammalian chromosome break repair.Nicole BennardoAnita ChengNick HuangJeremy M StarkCharacterizing the functional overlap and mutagenic potential of different pathways of chromosomal double-strand break (DSB) repair is important to understand how mutations arise during cancer development and treatment. To this end, we have compared the role of individual factors in three different pathways of mammalian DSB repair: alternative-nonhomologous end joining (alt-NHEJ), single-strand annealing (SSA), and homology directed repair (HDR/GC). Considering early steps of repair, we found that the DSB end-processing factors KU and CtIP affect all three pathways similarly, in that repair is suppressed by KU and promoted by CtIP. In contrast, both KU and CtIP appear dispensable for the absolute level of total-NHEJ between two tandem I-SceI-induced DSBs. During later steps of repair, we find that while the annealing and processing factors RAD52 and ERCC1 are important to promote SSA, both HDR/GC and alt-NHEJ are significantly less dependent upon these factors. As well, while disruption of RAD51 causes a decrease in HDR/GC and an increase in SSA, inhibition of this factor did not affect alt-NHEJ. These results suggest that the regulation of DSB end-processing via KU/CtIP is a common step during alt-NHEJ, SSA, and HDR/GC. However, at later steps of repair, alt-NHEJ is a mechanistically distinct pathway of DSB repair, and thus may play a unique role in mutagenesis during cancer development and therapy.https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1000110&type=printable |
| spellingShingle | Nicole Bennardo Anita Cheng Nick Huang Jeremy M Stark Alternative-NHEJ is a mechanistically distinct pathway of mammalian chromosome break repair. PLoS Genetics |
| title | Alternative-NHEJ is a mechanistically distinct pathway of mammalian chromosome break repair. |
| title_full | Alternative-NHEJ is a mechanistically distinct pathway of mammalian chromosome break repair. |
| title_fullStr | Alternative-NHEJ is a mechanistically distinct pathway of mammalian chromosome break repair. |
| title_full_unstemmed | Alternative-NHEJ is a mechanistically distinct pathway of mammalian chromosome break repair. |
| title_short | Alternative-NHEJ is a mechanistically distinct pathway of mammalian chromosome break repair. |
| title_sort | alternative nhej is a mechanistically distinct pathway of mammalian chromosome break repair |
| url | https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1000110&type=printable |
| work_keys_str_mv | AT nicolebennardo alternativenhejisamechanisticallydistinctpathwayofmammalianchromosomebreakrepair AT anitacheng alternativenhejisamechanisticallydistinctpathwayofmammalianchromosomebreakrepair AT nickhuang alternativenhejisamechanisticallydistinctpathwayofmammalianchromosomebreakrepair AT jeremymstark alternativenhejisamechanisticallydistinctpathwayofmammalianchromosomebreakrepair |