Establishment of a survival rabbit model for laryngotracheal stenosis: A prospective randomized study
Abstract Objective To develop a reproducible survival rabbit model for laryngotracheal stenosis (LTS). Methods Seventy New Zealand white (NZW) rabbits were randomly divided into experimental groups (n = 30) and a control group (n = 40). In experimental groups, a nylon brush was inserted retrograde f...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2024-12-01
|
| Series: | Laryngoscope Investigative Otolaryngology |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/lio2.70047 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850243885449609216 |
|---|---|
| author | Wei Chen Qingyu Wang Hongming Xu Yuhui Xie Lina Zhang Yao Li Guofeng Yan Yiwen Ding Shunkai Lu Zhibo Xie Jiarui Chen Mengrou Xu Xiaoben Liang Juan Chen Penghuai Fu Xiaoyan Li Liming Peng |
| author_facet | Wei Chen Qingyu Wang Hongming Xu Yuhui Xie Lina Zhang Yao Li Guofeng Yan Yiwen Ding Shunkai Lu Zhibo Xie Jiarui Chen Mengrou Xu Xiaoben Liang Juan Chen Penghuai Fu Xiaoyan Li Liming Peng |
| author_sort | Wei Chen |
| collection | DOAJ |
| description | Abstract Objective To develop a reproducible survival rabbit model for laryngotracheal stenosis (LTS). Methods Seventy New Zealand white (NZW) rabbits were randomly divided into experimental groups (n = 30) and a control group (n = 40). In experimental groups, a nylon brush was inserted retrograde from the tracheotomy through the subglottis and rotated until a full layer circumferential mucosal injury to cartilage exposure, assisted by fiberoptic laryngoscopy (FOL) visualization. Experimental group 1 (n = 10), rotated 10 times; group 2 (n = 20), rotated 20 times. The control group underwent tracheotomy only without nylon brush scraping. The rabbits underwent FOL at 1st, 4th, 8th, and 12th week postinjury respectively to observe the formation of LTS. They were euthanized and the larynxes and tracheas were subjected to gross and histopathological examination at 12 weeks postinjury. Results The control group all survived, while five cases in experimental groups died from LTS and/or mucous plug. Histological observation showed that the control group had intact laryngotracheal mucosal epithelium without any stenosis; the experimental groups showed proliferation of fibroblasts and thickening of collagen fibers. The mean stenosis in control group was 9.31 ± 0.98%, while that in experimental group 1 was 32.78 ± 7.07% and 58.25 ± 8.96% in experimental group 2. The difference between the three groups was statistically significant (χ2 = 47.98, p < .05). Conclusions We successfully developed a reproducible survival rabbit model for LTS using a nylon brush through FOL visualization combined with tracheostomy. This model can provide a mature and stable animal model for the exploration of wound‐healing pathophysiology and the effect of interventions. Level of evidence NA. |
| format | Article |
| id | doaj-art-8445c481fb624dffb1d62d70a5b1387b |
| institution | OA Journals |
| issn | 2378-8038 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Laryngoscope Investigative Otolaryngology |
| spelling | doaj-art-8445c481fb624dffb1d62d70a5b1387b2025-08-20T01:59:53ZengWileyLaryngoscope Investigative Otolaryngology2378-80382024-12-0196n/an/a10.1002/lio2.70047Establishment of a survival rabbit model for laryngotracheal stenosis: A prospective randomized studyWei Chen0Qingyu Wang1Hongming Xu2Yuhui Xie3Lina Zhang4Yao Li5Guofeng Yan6Yiwen Ding7Shunkai Lu8Zhibo Xie9Jiarui Chen10Mengrou Xu11Xiaoben Liang12Juan Chen13Penghuai Fu14Xiaoyan Li15Liming Peng16Department of Otolaryngology‐Head and Neck Surgery, Shanghai Children's Hospital, School of Medicine Shanghai Jiao Tong University Shanghai ChinaDepartment of Pathology, Shanghai Children's Hospital, School of Medicine Shanghai Jiao Tong University Shanghai ChinaDepartment of Otolaryngology‐Head and Neck Surgery, Shanghai Children's Hospital, School of Medicine Shanghai Jiao Tong University Shanghai ChinaDepartment of Otolaryngology‐Head and Neck Surgery, Huashan Hospital Fudan University Shanghai ChinaDepartment of Medical Statistics, School of Medicine Shanghai Jiaotong University Shanghai ChinaDepartment of Laboratory Animal Sciences, School of Medicine Shanghai Jiaotong University Shanghai ChinaDepartment of Laboratory Animal Sciences, School of Medicine Shanghai Jiaotong University Shanghai ChinaNational Engineering Research Center of Light Alloy Net Forming, State Key Laboratory of Metal Matrix Composites, School of Materials Science and Engineering Shanghai Jiao Tong University Shanghai ChinaDepartment of Laboratory Animal Sciences, School of Medicine Shanghai Jiaotong University Shanghai ChinaDepartment of Otolaryngology‐Head and Neck Surgery, Shanghai Children's Hospital, School of Medicine Shanghai Jiao Tong University Shanghai ChinaDepartment of Otolaryngology‐Head and Neck Surgery, Shanghai Children's Hospital, School of Medicine Shanghai Jiao Tong University Shanghai ChinaDepartment of Otolaryngology‐Head and Neck Surgery, Shanghai Children's Hospital, School of Medicine Shanghai Jiao Tong University Shanghai ChinaDepartment of Otolaryngology‐Head and Neck Surgery, Shanghai Children's Hospital, School of Medicine Shanghai Jiao Tong University Shanghai ChinaNational Engineering Research Center of Light Alloy Net Forming, State Key Laboratory of Metal Matrix Composites, School of Materials Science and Engineering Shanghai Jiao Tong University Shanghai ChinaNational Engineering Research Center of Light Alloy Net Forming, State Key Laboratory of Metal Matrix Composites, School of Materials Science and Engineering Shanghai Jiao Tong University Shanghai ChinaDepartment of Otolaryngology‐Head and Neck Surgery, Shanghai Children's Hospital, School of Medicine Shanghai Jiao Tong University Shanghai ChinaNational Engineering Research Center of Light Alloy Net Forming, State Key Laboratory of Metal Matrix Composites, School of Materials Science and Engineering Shanghai Jiao Tong University Shanghai ChinaAbstract Objective To develop a reproducible survival rabbit model for laryngotracheal stenosis (LTS). Methods Seventy New Zealand white (NZW) rabbits were randomly divided into experimental groups (n = 30) and a control group (n = 40). In experimental groups, a nylon brush was inserted retrograde from the tracheotomy through the subglottis and rotated until a full layer circumferential mucosal injury to cartilage exposure, assisted by fiberoptic laryngoscopy (FOL) visualization. Experimental group 1 (n = 10), rotated 10 times; group 2 (n = 20), rotated 20 times. The control group underwent tracheotomy only without nylon brush scraping. The rabbits underwent FOL at 1st, 4th, 8th, and 12th week postinjury respectively to observe the formation of LTS. They were euthanized and the larynxes and tracheas were subjected to gross and histopathological examination at 12 weeks postinjury. Results The control group all survived, while five cases in experimental groups died from LTS and/or mucous plug. Histological observation showed that the control group had intact laryngotracheal mucosal epithelium without any stenosis; the experimental groups showed proliferation of fibroblasts and thickening of collagen fibers. The mean stenosis in control group was 9.31 ± 0.98%, while that in experimental group 1 was 32.78 ± 7.07% and 58.25 ± 8.96% in experimental group 2. The difference between the three groups was statistically significant (χ2 = 47.98, p < .05). Conclusions We successfully developed a reproducible survival rabbit model for LTS using a nylon brush through FOL visualization combined with tracheostomy. This model can provide a mature and stable animal model for the exploration of wound‐healing pathophysiology and the effect of interventions. Level of evidence NA.https://doi.org/10.1002/lio2.70047fiberoptic laryngoscopylaryngotracheal stenosisNew Zealand white rabbitnylon brush scrapingtracheotomy |
| spellingShingle | Wei Chen Qingyu Wang Hongming Xu Yuhui Xie Lina Zhang Yao Li Guofeng Yan Yiwen Ding Shunkai Lu Zhibo Xie Jiarui Chen Mengrou Xu Xiaoben Liang Juan Chen Penghuai Fu Xiaoyan Li Liming Peng Establishment of a survival rabbit model for laryngotracheal stenosis: A prospective randomized study Laryngoscope Investigative Otolaryngology fiberoptic laryngoscopy laryngotracheal stenosis New Zealand white rabbit nylon brush scraping tracheotomy |
| title | Establishment of a survival rabbit model for laryngotracheal stenosis: A prospective randomized study |
| title_full | Establishment of a survival rabbit model for laryngotracheal stenosis: A prospective randomized study |
| title_fullStr | Establishment of a survival rabbit model for laryngotracheal stenosis: A prospective randomized study |
| title_full_unstemmed | Establishment of a survival rabbit model for laryngotracheal stenosis: A prospective randomized study |
| title_short | Establishment of a survival rabbit model for laryngotracheal stenosis: A prospective randomized study |
| title_sort | establishment of a survival rabbit model for laryngotracheal stenosis a prospective randomized study |
| topic | fiberoptic laryngoscopy laryngotracheal stenosis New Zealand white rabbit nylon brush scraping tracheotomy |
| url | https://doi.org/10.1002/lio2.70047 |
| work_keys_str_mv | AT weichen establishmentofasurvivalrabbitmodelforlaryngotrachealstenosisaprospectiverandomizedstudy AT qingyuwang establishmentofasurvivalrabbitmodelforlaryngotrachealstenosisaprospectiverandomizedstudy AT hongmingxu establishmentofasurvivalrabbitmodelforlaryngotrachealstenosisaprospectiverandomizedstudy AT yuhuixie establishmentofasurvivalrabbitmodelforlaryngotrachealstenosisaprospectiverandomizedstudy AT linazhang establishmentofasurvivalrabbitmodelforlaryngotrachealstenosisaprospectiverandomizedstudy AT yaoli establishmentofasurvivalrabbitmodelforlaryngotrachealstenosisaprospectiverandomizedstudy AT guofengyan establishmentofasurvivalrabbitmodelforlaryngotrachealstenosisaprospectiverandomizedstudy AT yiwending establishmentofasurvivalrabbitmodelforlaryngotrachealstenosisaprospectiverandomizedstudy AT shunkailu establishmentofasurvivalrabbitmodelforlaryngotrachealstenosisaprospectiverandomizedstudy AT zhiboxie establishmentofasurvivalrabbitmodelforlaryngotrachealstenosisaprospectiverandomizedstudy AT jiaruichen establishmentofasurvivalrabbitmodelforlaryngotrachealstenosisaprospectiverandomizedstudy AT mengrouxu establishmentofasurvivalrabbitmodelforlaryngotrachealstenosisaprospectiverandomizedstudy AT xiaobenliang establishmentofasurvivalrabbitmodelforlaryngotrachealstenosisaprospectiverandomizedstudy AT juanchen establishmentofasurvivalrabbitmodelforlaryngotrachealstenosisaprospectiverandomizedstudy AT penghuaifu establishmentofasurvivalrabbitmodelforlaryngotrachealstenosisaprospectiverandomizedstudy AT xiaoyanli establishmentofasurvivalrabbitmodelforlaryngotrachealstenosisaprospectiverandomizedstudy AT limingpeng establishmentofasurvivalrabbitmodelforlaryngotrachealstenosisaprospectiverandomizedstudy |