Effective DNA fragmentation technique for simple sequence repeat detection with a microsatellite-enriched library and high-throughput sequencing
Two different techniques for genomic DNA fragmentation before microsatellite-enriched library construction—restriction enzyme (NlaIII and MseI) digestion and sonication—were compared to examine their effects on simple sequence repeat (SSR) detection using high-throughput sequencing. Tens of thousand...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2017-04-01
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| Series: | BioTechniques |
| Subjects: | |
| Online Access: | https://www.future-science.com/doi/10.2144/000114536 |
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