Altered Expressions of miR-1238-3p, miR-494, miR-6069, and miR-139-3p in the Formation of Chronic Brucellosis
Brucellosis is a zoonotic disease that is still endemic in developing countries. Despite early diagnosis and treatment of patients, chronic infections are seen in 10–30% of patients. In this study, we aimed to investigate the immunological factors that play roles in the transition of brucellosis fro...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2016/4591468 |
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| author | Ferah Budak Salih Haldun Bal Gulcin Tezcan Halis Akalın Guher Goral Haluk Barbaros Oral |
| author_facet | Ferah Budak Salih Haldun Bal Gulcin Tezcan Halis Akalın Guher Goral Haluk Barbaros Oral |
| author_sort | Ferah Budak |
| collection | DOAJ |
| description | Brucellosis is a zoonotic disease that is still endemic in developing countries. Despite early diagnosis and treatment of patients, chronic infections are seen in 10–30% of patients. In this study, we aimed to investigate the immunological factors that play roles in the transition of brucellosis from acute infection into chronic infection. Here, more than 2000 miRNAs were screened in peripheral blood mononuclear cells (PBMCs) of patients with acute or chronic brucellosis and healthy controls by using miRNA array, and the results of the miRNA array were validated through qRT-PCR. Findings were evaluated using GeneSpring GX (Agilent) 13.0 software and KEGG pathway analysis. Four miRNAs were expressed in the chronic group but were not expressed in acute and control groups. Among these miRNAs, the expression level of miR-1238-3p was increased while miR-494, miR-6069, and miR-139-3p were decreased (p<0.05, fold change > 2). These miRNAs have the potential to be markers for chronic cases. The differentially expressed miRNAs and their predicted target genes involved in endocytosis, regulation of actin cytoskeleton, MAPK signaling pathway, and cytokine-cytokine receptor interaction and its chemokine signaling pathway indicate their potential roles in chronic brucellosis and its progression. It is the first study of miRNA expression analysis of human PBMC to clarify the mechanism of inveteracy in brucellosis. |
| format | Article |
| id | doaj-art-8436cfc9bb13466c8cbce91be0187614 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-8436cfc9bb13466c8cbce91be01876142025-02-03T05:48:26ZengWileyJournal of Immunology Research2314-88612314-71562016-01-01201610.1155/2016/45914684591468Altered Expressions of miR-1238-3p, miR-494, miR-6069, and miR-139-3p in the Formation of Chronic BrucellosisFerah Budak0Salih Haldun Bal1Gulcin Tezcan2Halis Akalın3Guher Goral4Haluk Barbaros Oral5Department of Immunology, Faculty of Medicine, Uludag University, Bursa, TurkeyDepartment of Immunology, Faculty of Medicine, Uludag University, Bursa, TurkeyDepartment of Medical Biology, Faculty of Medicine, Uludag University, Bursa, TurkeyDepartment of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, Uludag University, Bursa, TurkeyDepartment of Medical Microbiology, Faculty of Medicine, Uludag University, Bursa, TurkeyDepartment of Immunology, Faculty of Medicine, Uludag University, Bursa, TurkeyBrucellosis is a zoonotic disease that is still endemic in developing countries. Despite early diagnosis and treatment of patients, chronic infections are seen in 10–30% of patients. In this study, we aimed to investigate the immunological factors that play roles in the transition of brucellosis from acute infection into chronic infection. Here, more than 2000 miRNAs were screened in peripheral blood mononuclear cells (PBMCs) of patients with acute or chronic brucellosis and healthy controls by using miRNA array, and the results of the miRNA array were validated through qRT-PCR. Findings were evaluated using GeneSpring GX (Agilent) 13.0 software and KEGG pathway analysis. Four miRNAs were expressed in the chronic group but were not expressed in acute and control groups. Among these miRNAs, the expression level of miR-1238-3p was increased while miR-494, miR-6069, and miR-139-3p were decreased (p<0.05, fold change > 2). These miRNAs have the potential to be markers for chronic cases. The differentially expressed miRNAs and their predicted target genes involved in endocytosis, regulation of actin cytoskeleton, MAPK signaling pathway, and cytokine-cytokine receptor interaction and its chemokine signaling pathway indicate their potential roles in chronic brucellosis and its progression. It is the first study of miRNA expression analysis of human PBMC to clarify the mechanism of inveteracy in brucellosis.http://dx.doi.org/10.1155/2016/4591468 |
| spellingShingle | Ferah Budak Salih Haldun Bal Gulcin Tezcan Halis Akalın Guher Goral Haluk Barbaros Oral Altered Expressions of miR-1238-3p, miR-494, miR-6069, and miR-139-3p in the Formation of Chronic Brucellosis Journal of Immunology Research |
| title | Altered Expressions of miR-1238-3p, miR-494, miR-6069, and miR-139-3p in the Formation of Chronic Brucellosis |
| title_full | Altered Expressions of miR-1238-3p, miR-494, miR-6069, and miR-139-3p in the Formation of Chronic Brucellosis |
| title_fullStr | Altered Expressions of miR-1238-3p, miR-494, miR-6069, and miR-139-3p in the Formation of Chronic Brucellosis |
| title_full_unstemmed | Altered Expressions of miR-1238-3p, miR-494, miR-6069, and miR-139-3p in the Formation of Chronic Brucellosis |
| title_short | Altered Expressions of miR-1238-3p, miR-494, miR-6069, and miR-139-3p in the Formation of Chronic Brucellosis |
| title_sort | altered expressions of mir 1238 3p mir 494 mir 6069 and mir 139 3p in the formation of chronic brucellosis |
| url | http://dx.doi.org/10.1155/2016/4591468 |
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