A Retrospective Study on the Prevalence and Antimicrobial Susceptibility of Gram-Positive Cocci in a Pediatric Department: A Single-Center Report from Egypt
<i>Background and Objectives</i>: The rising prevalence of drug-resistant organisms presents a significant challenge to healthcare, underscoring the importance of implementing effective antimicrobial stewardship programs. The success of these programs depends on access to accurate, evide...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-06-01
|
| Series: | Medicina |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1648-9144/61/6/1089 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849705985913913344 |
|---|---|
| author | Mona Moheyeldin AbdelHalim Shimaa A. Abdel Salam Marwa O. Elgendy Ahmed M. Abdel Hamied Sultan M. Alshahrani Ahmed R. N. Ibrahim Heba Sherif Abdel Aziz |
| author_facet | Mona Moheyeldin AbdelHalim Shimaa A. Abdel Salam Marwa O. Elgendy Ahmed M. Abdel Hamied Sultan M. Alshahrani Ahmed R. N. Ibrahim Heba Sherif Abdel Aziz |
| author_sort | Mona Moheyeldin AbdelHalim |
| collection | DOAJ |
| description | <i>Background and Objectives</i>: The rising prevalence of drug-resistant organisms presents a significant challenge to healthcare, underscoring the importance of implementing effective antimicrobial stewardship programs. The success of these programs depends on access to accurate, evidence-based data reflecting local patterns of antibiotic resistance. This study aims to assess the antimicrobial susceptibility profiles of gram-positive bacteria isolated from pediatric patients in a tertiary care hospital in Egypt. <i>Materials and Methods</i>: We carried out a retrospective study over a five-year period, from January 2018 to December 2022, using microbiology laboratory records. Clinical samples included blood, urine, respiratory secretions, pus, wound, cerebrospinal fluid (CSF), and pleural fluid. The analysis focused on the resistance patterns of gram-positive pathogens identified through routine culture procedures. Antimicrobial susceptibility testing was performed using the Kirby–Bauer disc diffusion method, and vancomycin MIC was confirmed using the VITEK 2 system. <i>Results</i>: A total of 3223 gram-positive bacterial isolates were identified. <i>Staphylococcus aureus</i>, including 82.5% methicillin-resistant strains (MRSA), exhibited high resistance to erythromycin (47.3%) and gentamicin (low potency) (32.1%). Coagulase-negative staphylococci (CoNS) showed the highest erythromycin resistance (up to 88.3%), while <i>Enterococcus</i> spp. demonstrated declining susceptibility to vancomycin, levofloxacin, and erythromycin. Across all isolates, vancomycin and gentamicin (high potency) showed the highest overall susceptibility. Resistance to cotrimoxazole and doxycycline declined over the five-year period. <i>Conclusions</i>: While a decline in resistance was noted for some agents, persistent resistance to key antibiotics (particularly erythromycin and gentamicin) among MRSA and CoNS remains concerning. These findings underscore the importance of targeted antimicrobial stewardship interventions and continuous surveillance to inform empirical therapy in pediatric settings. |
| format | Article |
| id | doaj-art-84351475d67e4c48bb7a28fb1aed7153 |
| institution | DOAJ |
| issn | 1010-660X 1648-9144 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Medicina |
| spelling | doaj-art-84351475d67e4c48bb7a28fb1aed71532025-08-20T03:16:19ZengMDPI AGMedicina1010-660X1648-91442025-06-01616108910.3390/medicina61061089A Retrospective Study on the Prevalence and Antimicrobial Susceptibility of Gram-Positive Cocci in a Pediatric Department: A Single-Center Report from EgyptMona Moheyeldin AbdelHalim0Shimaa A. Abdel Salam1Marwa O. Elgendy2Ahmed M. Abdel Hamied3Sultan M. Alshahrani4Ahmed R. N. Ibrahim5Heba Sherif Abdel Aziz6Department of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Cairo 12613, EgyptDepartment of Medical Microbiology and Immunology, Faculty of Medicine, Ain Shams University, Cairo 11566, EgyptDepartment of Clinical Pharmacy, Beni-Suef University Hospitals, Faculty of Medicine, Beni-Suef University, Beni-Suef 62521, EgyptDepartment of Pediatrics, Faculty of Medicine, Nahda University (NUB), Beni-Suef 62513, EgyptDepartment of Clinical Pharmacy, College of Pharmacy, King Khalid University, Abha 61421, Saudi ArabiaDepartment of Clinical Pharmacy, College of Pharmacy, King Khalid University, Abha 61421, Saudi ArabiaDepartment of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Cairo 12613, Egypt<i>Background and Objectives</i>: The rising prevalence of drug-resistant organisms presents a significant challenge to healthcare, underscoring the importance of implementing effective antimicrobial stewardship programs. The success of these programs depends on access to accurate, evidence-based data reflecting local patterns of antibiotic resistance. This study aims to assess the antimicrobial susceptibility profiles of gram-positive bacteria isolated from pediatric patients in a tertiary care hospital in Egypt. <i>Materials and Methods</i>: We carried out a retrospective study over a five-year period, from January 2018 to December 2022, using microbiology laboratory records. Clinical samples included blood, urine, respiratory secretions, pus, wound, cerebrospinal fluid (CSF), and pleural fluid. The analysis focused on the resistance patterns of gram-positive pathogens identified through routine culture procedures. Antimicrobial susceptibility testing was performed using the Kirby–Bauer disc diffusion method, and vancomycin MIC was confirmed using the VITEK 2 system. <i>Results</i>: A total of 3223 gram-positive bacterial isolates were identified. <i>Staphylococcus aureus</i>, including 82.5% methicillin-resistant strains (MRSA), exhibited high resistance to erythromycin (47.3%) and gentamicin (low potency) (32.1%). Coagulase-negative staphylococci (CoNS) showed the highest erythromycin resistance (up to 88.3%), while <i>Enterococcus</i> spp. demonstrated declining susceptibility to vancomycin, levofloxacin, and erythromycin. Across all isolates, vancomycin and gentamicin (high potency) showed the highest overall susceptibility. Resistance to cotrimoxazole and doxycycline declined over the five-year period. <i>Conclusions</i>: While a decline in resistance was noted for some agents, persistent resistance to key antibiotics (particularly erythromycin and gentamicin) among MRSA and CoNS remains concerning. These findings underscore the importance of targeted antimicrobial stewardship interventions and continuous surveillance to inform empirical therapy in pediatric settings.https://www.mdpi.com/1648-9144/61/6/1089antimicrobial susceptibilitygram-positive bacteriapediatric hospital |
| spellingShingle | Mona Moheyeldin AbdelHalim Shimaa A. Abdel Salam Marwa O. Elgendy Ahmed M. Abdel Hamied Sultan M. Alshahrani Ahmed R. N. Ibrahim Heba Sherif Abdel Aziz A Retrospective Study on the Prevalence and Antimicrobial Susceptibility of Gram-Positive Cocci in a Pediatric Department: A Single-Center Report from Egypt Medicina antimicrobial susceptibility gram-positive bacteria pediatric hospital |
| title | A Retrospective Study on the Prevalence and Antimicrobial Susceptibility of Gram-Positive Cocci in a Pediatric Department: A Single-Center Report from Egypt |
| title_full | A Retrospective Study on the Prevalence and Antimicrobial Susceptibility of Gram-Positive Cocci in a Pediatric Department: A Single-Center Report from Egypt |
| title_fullStr | A Retrospective Study on the Prevalence and Antimicrobial Susceptibility of Gram-Positive Cocci in a Pediatric Department: A Single-Center Report from Egypt |
| title_full_unstemmed | A Retrospective Study on the Prevalence and Antimicrobial Susceptibility of Gram-Positive Cocci in a Pediatric Department: A Single-Center Report from Egypt |
| title_short | A Retrospective Study on the Prevalence and Antimicrobial Susceptibility of Gram-Positive Cocci in a Pediatric Department: A Single-Center Report from Egypt |
| title_sort | retrospective study on the prevalence and antimicrobial susceptibility of gram positive cocci in a pediatric department a single center report from egypt |
| topic | antimicrobial susceptibility gram-positive bacteria pediatric hospital |
| url | https://www.mdpi.com/1648-9144/61/6/1089 |
| work_keys_str_mv | AT monamoheyeldinabdelhalim aretrospectivestudyontheprevalenceandantimicrobialsusceptibilityofgrampositivecocciinapediatricdepartmentasinglecenterreportfromegypt AT shimaaaabdelsalam aretrospectivestudyontheprevalenceandantimicrobialsusceptibilityofgrampositivecocciinapediatricdepartmentasinglecenterreportfromegypt AT marwaoelgendy aretrospectivestudyontheprevalenceandantimicrobialsusceptibilityofgrampositivecocciinapediatricdepartmentasinglecenterreportfromegypt AT ahmedmabdelhamied aretrospectivestudyontheprevalenceandantimicrobialsusceptibilityofgrampositivecocciinapediatricdepartmentasinglecenterreportfromegypt AT sultanmalshahrani aretrospectivestudyontheprevalenceandantimicrobialsusceptibilityofgrampositivecocciinapediatricdepartmentasinglecenterreportfromegypt AT ahmedrnibrahim aretrospectivestudyontheprevalenceandantimicrobialsusceptibilityofgrampositivecocciinapediatricdepartmentasinglecenterreportfromegypt AT hebasherifabdelaziz aretrospectivestudyontheprevalenceandantimicrobialsusceptibilityofgrampositivecocciinapediatricdepartmentasinglecenterreportfromegypt AT monamoheyeldinabdelhalim retrospectivestudyontheprevalenceandantimicrobialsusceptibilityofgrampositivecocciinapediatricdepartmentasinglecenterreportfromegypt AT shimaaaabdelsalam retrospectivestudyontheprevalenceandantimicrobialsusceptibilityofgrampositivecocciinapediatricdepartmentasinglecenterreportfromegypt AT marwaoelgendy retrospectivestudyontheprevalenceandantimicrobialsusceptibilityofgrampositivecocciinapediatricdepartmentasinglecenterreportfromegypt AT ahmedmabdelhamied retrospectivestudyontheprevalenceandantimicrobialsusceptibilityofgrampositivecocciinapediatricdepartmentasinglecenterreportfromegypt AT sultanmalshahrani retrospectivestudyontheprevalenceandantimicrobialsusceptibilityofgrampositivecocciinapediatricdepartmentasinglecenterreportfromegypt AT ahmedrnibrahim retrospectivestudyontheprevalenceandantimicrobialsusceptibilityofgrampositivecocciinapediatricdepartmentasinglecenterreportfromegypt AT hebasherifabdelaziz retrospectivestudyontheprevalenceandantimicrobialsusceptibilityofgrampositivecocciinapediatricdepartmentasinglecenterreportfromegypt |