Malaria bivalent viral vectored vaccine protects against Plasmodium falciparum and vivax and blocks parasite transmission
Abstract Malaria remains a major infectious disease, with Plasmodium falciparum and Plasmodium vivax often co-endemic, requiring a dual-target vaccine for adequate control. We previously developed monovalent vaccines against P. falciparum or P. vivax using vaccinia virus LC16m8Δ (m8Δ) and adeno-asso...
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Nature Portfolio
2025-07-01
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| Series: | npj Vaccines |
| Online Access: | https://doi.org/10.1038/s41541-025-01229-3 |
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| author | Yutaro Yamamoto Takuto Katayama Camila Fabbri Sora Niwa Daiki Okuhara Mitsuhiro Iyori Ammar A. Hasyim Hiroaki Mizukami Hisatoshi Shida Stefanie Lopes Shigeto Yoshida |
| author_facet | Yutaro Yamamoto Takuto Katayama Camila Fabbri Sora Niwa Daiki Okuhara Mitsuhiro Iyori Ammar A. Hasyim Hiroaki Mizukami Hisatoshi Shida Stefanie Lopes Shigeto Yoshida |
| author_sort | Yutaro Yamamoto |
| collection | DOAJ |
| description | Abstract Malaria remains a major infectious disease, with Plasmodium falciparum and Plasmodium vivax often co-endemic, requiring a dual-target vaccine for adequate control. We previously developed monovalent vaccines against P. falciparum or P. vivax using vaccinia virus LC16m8Δ (m8Δ) and adeno-associated virus type 1 (AAV1). Here, we demonstrate the efficacy of a novel bivalent malaria vaccine against P. falciparum and P. vivax. The m8Δ vaccine harbors two gene cassettes encoding Pfs25-PfCSP and Pvs25-PvCSP fusion proteins, while the AAV1 vaccine includes two recombinant AAV1s carrying one of these cassettes as a mixture. Heterologous m8Δ-prime and AAV1-boost immunization provided 70% protection against both PfCSP/Pb and PvCSP/Pb transgenic sporozoites. Moreover, a membrane feeding assay using P. vivax isolates from infected patients in the Brazilian Amazon showed 90% transmission-blocking efficacy. The bivalent vaccine outperformed monovalent combinations, maintaining immune responses for over 7 months, and shows promise for malaria control and elimination. |
| format | Article |
| id | doaj-art-8433ff13df5c49b1a3a8d57bea351374 |
| institution | Kabale University |
| issn | 2059-0105 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Vaccines |
| spelling | doaj-art-8433ff13df5c49b1a3a8d57bea3513742025-08-20T03:45:44ZengNature Portfolionpj Vaccines2059-01052025-07-0110111210.1038/s41541-025-01229-3Malaria bivalent viral vectored vaccine protects against Plasmodium falciparum and vivax and blocks parasite transmissionYutaro Yamamoto0Takuto Katayama1Camila Fabbri2Sora Niwa3Daiki Okuhara4Mitsuhiro Iyori5Ammar A. Hasyim6Hiroaki Mizukami7Hisatoshi Shida8Stefanie Lopes9Shigeto Yoshida10Laboratory of Vaccinology and Applied Immunology, Kanazawa University School of Pharmacy, Kanazawa UniversityLaboratory of Vaccinology and Applied Immunology, Kanazawa University School of Pharmacy, Kanazawa UniversityInstituto Leônidas & Maria Deane-Fiocruz Amazônia, Laboratório de Diagnóstico e Controle e Doenças Infecciosas da AmazôniaLaboratory of Vaccinology and Applied Immunology, Kanazawa University School of Pharmacy, Kanazawa UniversityLaboratory of Vaccinology and Applied Immunology, Kanazawa University School of Pharmacy, Kanazawa UniversityResearch Institute of Pharmaceutical Sciences, Musashino UniversityLaboratory of Vaccinology and Applied Immunology, Kanazawa University School of Pharmacy, Kanazawa UniversityDivision of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical UniversityLaboratory of Primate Model, Research Center for Infectious Diseases, Institute for Frontier Life and Medical Science, Kyoto UniversityInstituto Leônidas & Maria Deane-Fiocruz Amazônia, Laboratório de Diagnóstico e Controle e Doenças Infecciosas da AmazôniaLaboratory of Vaccinology and Applied Immunology, Kanazawa University School of Pharmacy, Kanazawa UniversityAbstract Malaria remains a major infectious disease, with Plasmodium falciparum and Plasmodium vivax often co-endemic, requiring a dual-target vaccine for adequate control. We previously developed monovalent vaccines against P. falciparum or P. vivax using vaccinia virus LC16m8Δ (m8Δ) and adeno-associated virus type 1 (AAV1). Here, we demonstrate the efficacy of a novel bivalent malaria vaccine against P. falciparum and P. vivax. The m8Δ vaccine harbors two gene cassettes encoding Pfs25-PfCSP and Pvs25-PvCSP fusion proteins, while the AAV1 vaccine includes two recombinant AAV1s carrying one of these cassettes as a mixture. Heterologous m8Δ-prime and AAV1-boost immunization provided 70% protection against both PfCSP/Pb and PvCSP/Pb transgenic sporozoites. Moreover, a membrane feeding assay using P. vivax isolates from infected patients in the Brazilian Amazon showed 90% transmission-blocking efficacy. The bivalent vaccine outperformed monovalent combinations, maintaining immune responses for over 7 months, and shows promise for malaria control and elimination.https://doi.org/10.1038/s41541-025-01229-3 |
| spellingShingle | Yutaro Yamamoto Takuto Katayama Camila Fabbri Sora Niwa Daiki Okuhara Mitsuhiro Iyori Ammar A. Hasyim Hiroaki Mizukami Hisatoshi Shida Stefanie Lopes Shigeto Yoshida Malaria bivalent viral vectored vaccine protects against Plasmodium falciparum and vivax and blocks parasite transmission npj Vaccines |
| title | Malaria bivalent viral vectored vaccine protects against Plasmodium falciparum and vivax and blocks parasite transmission |
| title_full | Malaria bivalent viral vectored vaccine protects against Plasmodium falciparum and vivax and blocks parasite transmission |
| title_fullStr | Malaria bivalent viral vectored vaccine protects against Plasmodium falciparum and vivax and blocks parasite transmission |
| title_full_unstemmed | Malaria bivalent viral vectored vaccine protects against Plasmodium falciparum and vivax and blocks parasite transmission |
| title_short | Malaria bivalent viral vectored vaccine protects against Plasmodium falciparum and vivax and blocks parasite transmission |
| title_sort | malaria bivalent viral vectored vaccine protects against plasmodium falciparum and vivax and blocks parasite transmission |
| url | https://doi.org/10.1038/s41541-025-01229-3 |
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