High expressions of BCL6 and Lewis y antigen are correlated with high tumor burden and poor prognosis in epithelial ovarian cancer

Aberrant regulation of BCL6 plays crucial oncogenic roles in various malignant tumors; howbeit, the function of BCL6 in tumorigenesis of ovarian cancer remains unclear. The aim of this study is to investigate the role of BCL6 in ovarian cancer. The methods of immunohistochemical staining, quantitati...

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Main Authors: Liancheng Zhu, Huilin Feng, Shan Jin, Mingzi Tan, Song Gao, Huiyu Zhuang, Zhenhua Hu, Huimin Wang, Zuofei Song, Bei Lin
Format: Article
Language:English
Published: SAGE Publishing 2017-06-01
Series:Tumor Biology
Online Access:https://doi.org/10.1177/1010428317711655
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author Liancheng Zhu
Huilin Feng
Shan Jin
Mingzi Tan
Song Gao
Huiyu Zhuang
Zhenhua Hu
Huimin Wang
Zuofei Song
Bei Lin
author_facet Liancheng Zhu
Huilin Feng
Shan Jin
Mingzi Tan
Song Gao
Huiyu Zhuang
Zhenhua Hu
Huimin Wang
Zuofei Song
Bei Lin
author_sort Liancheng Zhu
collection DOAJ
description Aberrant regulation of BCL6 plays crucial oncogenic roles in various malignant tumors; howbeit, the function of BCL6 in tumorigenesis of ovarian cancer remains unclear. The aim of this study is to investigate the role of BCL6 in ovarian cancer. The methods of immunohistochemical staining, quantitative real-time polymerase chain reaction, immunocytochemical staining, and gene expression profile enrichment analysis were performed to identify the possible role of BCL6 in ovarian cancer. We observed that the expression of BCL6 was significantly higher in ovarian cancer tissues and correlated with higher tumor burden including advanced International Federation of Gynecology and Obstetrics stages, poor differentiation, Type II ovarian cancer, the presence of >1 cm residual tumor size, and appearance of recurrence or death (all p  < 0.05). The expression patterns of Lewis y were similar to these of BCL6. Multivariate Cox analysis demonstrated that advanced International Federation of Gynecology and Obstetrics stage, lymph node metastasis, residual tumor size >1 cm, as well as high expressions of BCL6 and Lewis y antigen were independent factors of worse progression-free survival and overall survival (all p  < 0.05). There was a positive correlation of the expressions of BCL6 and Lewis y antigen. The associated genes with BCL6 in response to Lewis y antigen were identified, including four upregulated genes ( SOCS3, STAT1, PPARG , and GADD45A ) and three downregulated genes ( ACAN, E2F3 , and ZBTB7B ). In conclusion, the high expressions of BCL6 and Lewis y antigen are associated with development, high tumor burden, and worse prognosis of ovarian cancer and targeting BCL6 could be a novel therapeutic strategy for ovarian cancer treatment.
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spelling doaj-art-8424cce2cf934fcab95cd5da0a064bc92025-08-20T03:34:49ZengSAGE PublishingTumor Biology1423-03802017-06-013910.1177/1010428317711655High expressions of BCL6 and Lewis y antigen are correlated with high tumor burden and poor prognosis in epithelial ovarian cancerLiancheng Zhu0Huilin Feng1Shan Jin2Mingzi Tan3Song Gao4Huiyu Zhuang5Zhenhua Hu6Huimin Wang7Zuofei Song8Bei Lin9Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, Shenyang, ChinaThe Third People’s Hospital of Liaoyang City, Liaoyang, ChinaDepartment of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, Shenyang, ChinaDepartment of Gynecology, Liaoning Cancer Hospital, Shenyang, ChinaDepartment of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, Shenyang, ChinaDepartment of Obstetrics & Gynecology, Beijing Chao-Yang Hospital Affiliated to Capital Medical University, Beijing, ChinaDepartment of Obstetrics and Gynecology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Gynecology, Liaoning Cancer Hospital, Shenyang, ChinaDepartment of Obstetrics and Gynecology, China General Hospital of Shenyang Military Region, Shenyang, ChinaDepartment of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, Shenyang, ChinaAberrant regulation of BCL6 plays crucial oncogenic roles in various malignant tumors; howbeit, the function of BCL6 in tumorigenesis of ovarian cancer remains unclear. The aim of this study is to investigate the role of BCL6 in ovarian cancer. The methods of immunohistochemical staining, quantitative real-time polymerase chain reaction, immunocytochemical staining, and gene expression profile enrichment analysis were performed to identify the possible role of BCL6 in ovarian cancer. We observed that the expression of BCL6 was significantly higher in ovarian cancer tissues and correlated with higher tumor burden including advanced International Federation of Gynecology and Obstetrics stages, poor differentiation, Type II ovarian cancer, the presence of >1 cm residual tumor size, and appearance of recurrence or death (all p  < 0.05). The expression patterns of Lewis y were similar to these of BCL6. Multivariate Cox analysis demonstrated that advanced International Federation of Gynecology and Obstetrics stage, lymph node metastasis, residual tumor size >1 cm, as well as high expressions of BCL6 and Lewis y antigen were independent factors of worse progression-free survival and overall survival (all p  < 0.05). There was a positive correlation of the expressions of BCL6 and Lewis y antigen. The associated genes with BCL6 in response to Lewis y antigen were identified, including four upregulated genes ( SOCS3, STAT1, PPARG , and GADD45A ) and three downregulated genes ( ACAN, E2F3 , and ZBTB7B ). In conclusion, the high expressions of BCL6 and Lewis y antigen are associated with development, high tumor burden, and worse prognosis of ovarian cancer and targeting BCL6 could be a novel therapeutic strategy for ovarian cancer treatment.https://doi.org/10.1177/1010428317711655
spellingShingle Liancheng Zhu
Huilin Feng
Shan Jin
Mingzi Tan
Song Gao
Huiyu Zhuang
Zhenhua Hu
Huimin Wang
Zuofei Song
Bei Lin
High expressions of BCL6 and Lewis y antigen are correlated with high tumor burden and poor prognosis in epithelial ovarian cancer
Tumor Biology
title High expressions of BCL6 and Lewis y antigen are correlated with high tumor burden and poor prognosis in epithelial ovarian cancer
title_full High expressions of BCL6 and Lewis y antigen are correlated with high tumor burden and poor prognosis in epithelial ovarian cancer
title_fullStr High expressions of BCL6 and Lewis y antigen are correlated with high tumor burden and poor prognosis in epithelial ovarian cancer
title_full_unstemmed High expressions of BCL6 and Lewis y antigen are correlated with high tumor burden and poor prognosis in epithelial ovarian cancer
title_short High expressions of BCL6 and Lewis y antigen are correlated with high tumor burden and poor prognosis in epithelial ovarian cancer
title_sort high expressions of bcl6 and lewis y antigen are correlated with high tumor burden and poor prognosis in epithelial ovarian cancer
url https://doi.org/10.1177/1010428317711655
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