Dual‐Activatable Nano‐Immunomodulator for NIR‐II Fluorescence Imaging‐Guided Precision Cancer Photodynamic Immunotherapy

Abstract Photodynamic immunotherapy which combines photodynamic therapy with immunotherapy has become an important and effective method for the treatment of cancer. However, most cancer photodynamic immunotherapeutic systems are not able to achieve precise release of immunomodulators, resulting in s...

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Main Authors: Shanchao Diao, Zhifan Zhang, Sijun Zhao, Qiang Li, Xiaolong Zhang, Xiangqi Yang, Zhiwei Xu, Mingming Liu, Wen Zhou, Rutian Li, Chen Xie, Quli Fan
Format: Article
Language:English
Published: Wiley 2024-12-01
Series:Advanced Science
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Online Access:https://doi.org/10.1002/advs.202409833
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author Shanchao Diao
Zhifan Zhang
Sijun Zhao
Qiang Li
Xiaolong Zhang
Xiangqi Yang
Zhiwei Xu
Mingming Liu
Wen Zhou
Rutian Li
Chen Xie
Quli Fan
author_facet Shanchao Diao
Zhifan Zhang
Sijun Zhao
Qiang Li
Xiaolong Zhang
Xiangqi Yang
Zhiwei Xu
Mingming Liu
Wen Zhou
Rutian Li
Chen Xie
Quli Fan
author_sort Shanchao Diao
collection DOAJ
description Abstract Photodynamic immunotherapy which combines photodynamic therapy with immunotherapy has become an important and effective method for the treatment of cancer. However, most cancer photodynamic immunotherapeutic systems are not able to achieve precise release of immunomodulators, resulting in systemic side effects and poor patient outcomes. Herein, a dual‐activatable nano‐immunomodulator (DIR NP), which both its photodynamic effect and agonist release can be activated under specific stimuli, is reported for precision cancer photodynamic immunotherapy. The DIR NP is self‐assembled from an R848‐conjugated amphiphilic polymer (mPEG‐TK‐R848) and a hydrophobic oxidized bovine serum albumin (BSA‐SOH)‐conjugatable photosensitizer (DIR). DIR NPs may generate a small amount of 1O2 under 808 nm laser irradiation, leading to the cleavage of thioketal (TK) moiety and release of R848 and DIR. The released DIR may conjugate with tumor‐overexpressed BSA‐SOH, improving its photodynamic efficiency and NIR‐II fluorescence signal. Such photodynamic efficiency improvement may further enhance the release of cargoes upon irradiation. The activated photodynamic effect induces immunogenic cell death (ICD) to release immune factors and R848 can enhance the maturation of dendritic cells for inhibiting the growth of both primary and distant tumors and eliminating lung metastasis. Therefore, this study provides a dual‐activatable intelligent nano‐immunomodulator for precise regulation of tumor photodynamic immunotherapy.
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spelling doaj-art-841ccf4dfb3f403ca5e24887432c5c8d2025-08-20T02:19:01ZengWileyAdvanced Science2198-38442024-12-011145n/an/a10.1002/advs.202409833Dual‐Activatable Nano‐Immunomodulator for NIR‐II Fluorescence Imaging‐Guided Precision Cancer Photodynamic ImmunotherapyShanchao Diao0Zhifan Zhang1Sijun Zhao2Qiang Li3Xiaolong Zhang4Xiangqi Yang5Zhiwei Xu6Mingming Liu7Wen Zhou8Rutian Li9Chen Xie10Quli Fan11State Key Laboratory of Organic Electronics and Information Displays & Institute of Advanced Materials (IAM) Nanjing University of Posts & Telecommunications 9 Wenyuan Road Nanjing 210023 ChinaThe Comprehensive Cancer Centre of Nanjing Drum Tower Hospital, the Affiliated Hospital of Medical School Nanjing University Nanjing 210008 ChinaState Key Laboratory of Organic Electronics and Information Displays & Institute of Advanced Materials (IAM) Nanjing University of Posts & Telecommunications 9 Wenyuan Road Nanjing 210023 ChinaState Key Laboratory of Organic Electronics and Information Displays & Institute of Advanced Materials (IAM) Nanjing University of Posts & Telecommunications 9 Wenyuan Road Nanjing 210023 ChinaState Key Laboratory of Organic Electronics and Information Displays & Institute of Advanced Materials (IAM) Nanjing University of Posts & Telecommunications 9 Wenyuan Road Nanjing 210023 ChinaState Key Laboratory of Organic Electronics and Information Displays & Institute of Advanced Materials (IAM) Nanjing University of Posts & Telecommunications 9 Wenyuan Road Nanjing 210023 ChinaState Key Laboratory of Organic Electronics and Information Displays & Institute of Advanced Materials (IAM) Nanjing University of Posts & Telecommunications 9 Wenyuan Road Nanjing 210023 ChinaState Key Laboratory of Organic Electronics and Information Displays & Institute of Advanced Materials (IAM) Nanjing University of Posts & Telecommunications 9 Wenyuan Road Nanjing 210023 ChinaState Key Laboratory of Organic Electronics and Information Displays & Institute of Advanced Materials (IAM) Nanjing University of Posts & Telecommunications 9 Wenyuan Road Nanjing 210023 ChinaThe Comprehensive Cancer Centre of Nanjing Drum Tower Hospital, the Affiliated Hospital of Medical School Nanjing University Nanjing 210008 ChinaState Key Laboratory of Organic Electronics and Information Displays & Institute of Advanced Materials (IAM) Nanjing University of Posts & Telecommunications 9 Wenyuan Road Nanjing 210023 ChinaState Key Laboratory of Organic Electronics and Information Displays & Institute of Advanced Materials (IAM) Nanjing University of Posts & Telecommunications 9 Wenyuan Road Nanjing 210023 ChinaAbstract Photodynamic immunotherapy which combines photodynamic therapy with immunotherapy has become an important and effective method for the treatment of cancer. However, most cancer photodynamic immunotherapeutic systems are not able to achieve precise release of immunomodulators, resulting in systemic side effects and poor patient outcomes. Herein, a dual‐activatable nano‐immunomodulator (DIR NP), which both its photodynamic effect and agonist release can be activated under specific stimuli, is reported for precision cancer photodynamic immunotherapy. The DIR NP is self‐assembled from an R848‐conjugated amphiphilic polymer (mPEG‐TK‐R848) and a hydrophobic oxidized bovine serum albumin (BSA‐SOH)‐conjugatable photosensitizer (DIR). DIR NPs may generate a small amount of 1O2 under 808 nm laser irradiation, leading to the cleavage of thioketal (TK) moiety and release of R848 and DIR. The released DIR may conjugate with tumor‐overexpressed BSA‐SOH, improving its photodynamic efficiency and NIR‐II fluorescence signal. Such photodynamic efficiency improvement may further enhance the release of cargoes upon irradiation. The activated photodynamic effect induces immunogenic cell death (ICD) to release immune factors and R848 can enhance the maturation of dendritic cells for inhibiting the growth of both primary and distant tumors and eliminating lung metastasis. Therefore, this study provides a dual‐activatable intelligent nano‐immunomodulator for precise regulation of tumor photodynamic immunotherapy.https://doi.org/10.1002/advs.202409833NIR‐II fluorescence imagingphoto‐activationphotodynamic therapyphotoimmunotherapyphototheranostics
spellingShingle Shanchao Diao
Zhifan Zhang
Sijun Zhao
Qiang Li
Xiaolong Zhang
Xiangqi Yang
Zhiwei Xu
Mingming Liu
Wen Zhou
Rutian Li
Chen Xie
Quli Fan
Dual‐Activatable Nano‐Immunomodulator for NIR‐II Fluorescence Imaging‐Guided Precision Cancer Photodynamic Immunotherapy
Advanced Science
NIR‐II fluorescence imaging
photo‐activation
photodynamic therapy
photoimmunotherapy
phototheranostics
title Dual‐Activatable Nano‐Immunomodulator for NIR‐II Fluorescence Imaging‐Guided Precision Cancer Photodynamic Immunotherapy
title_full Dual‐Activatable Nano‐Immunomodulator for NIR‐II Fluorescence Imaging‐Guided Precision Cancer Photodynamic Immunotherapy
title_fullStr Dual‐Activatable Nano‐Immunomodulator for NIR‐II Fluorescence Imaging‐Guided Precision Cancer Photodynamic Immunotherapy
title_full_unstemmed Dual‐Activatable Nano‐Immunomodulator for NIR‐II Fluorescence Imaging‐Guided Precision Cancer Photodynamic Immunotherapy
title_short Dual‐Activatable Nano‐Immunomodulator for NIR‐II Fluorescence Imaging‐Guided Precision Cancer Photodynamic Immunotherapy
title_sort dual activatable nano immunomodulator for nir ii fluorescence imaging guided precision cancer photodynamic immunotherapy
topic NIR‐II fluorescence imaging
photo‐activation
photodynamic therapy
photoimmunotherapy
phototheranostics
url https://doi.org/10.1002/advs.202409833
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AT sijunzhao dualactivatablenanoimmunomodulatorforniriifluorescenceimagingguidedprecisioncancerphotodynamicimmunotherapy
AT qiangli dualactivatablenanoimmunomodulatorforniriifluorescenceimagingguidedprecisioncancerphotodynamicimmunotherapy
AT xiaolongzhang dualactivatablenanoimmunomodulatorforniriifluorescenceimagingguidedprecisioncancerphotodynamicimmunotherapy
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AT chenxie dualactivatablenanoimmunomodulatorforniriifluorescenceimagingguidedprecisioncancerphotodynamicimmunotherapy
AT qulifan dualactivatablenanoimmunomodulatorforniriifluorescenceimagingguidedprecisioncancerphotodynamicimmunotherapy