Antibodies against Clostridium butyricum in the children of mothers at risk for gestational diabetes

Gestational diabetes mellitus (GDM) is linked to an imbalance in gut microbiota composition, which can be transferred to the mother’s offspring. Clostridium butyricum, known for its health benefits in diabetes and allergy, lacks sufficient data regarding its effect on the immune system’s development...

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Main Authors: Celeste Peterson, Aili Tagoma, Kristi Alnek, Anu Bärenson, Tamara Vorobjova, Ija Talja, Helis Janson, Anne Kirss, Siiri Kõljalg, Aki Sinkkonen, Marja Irmeli Roslund, Raivo Uibo
Format: Article
Language:English
Published: Taylor & Francis Group 2025-05-01
Series:Immunological Medicine
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Online Access:https://www.tandfonline.com/doi/10.1080/25785826.2025.2504021
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author Celeste Peterson
Aili Tagoma
Kristi Alnek
Anu Bärenson
Tamara Vorobjova
Ija Talja
Helis Janson
Anne Kirss
Siiri Kõljalg
Aki Sinkkonen
Marja Irmeli Roslund
Raivo Uibo
author_facet Celeste Peterson
Aili Tagoma
Kristi Alnek
Anu Bärenson
Tamara Vorobjova
Ija Talja
Helis Janson
Anne Kirss
Siiri Kõljalg
Aki Sinkkonen
Marja Irmeli Roslund
Raivo Uibo
author_sort Celeste Peterson
collection DOAJ
description Gestational diabetes mellitus (GDM) is linked to an imbalance in gut microbiota composition, which can be transferred to the mother’s offspring. Clostridium butyricum, known for its health benefits in diabetes and allergy, lacks sufficient data regarding its effect on the immune system’s development in the offspring of mothers with GDM. This study assessed antibody responses against C. butyricum T2F3 in children of mothers at risk for GDM, involving 88 children aged 1–6 years. Antibody responses were measured with flow cytometry and immunoblot. Lower IgG median fluorescence intensity (MFI) values and fewer IgA and IgG bands against C. butyricum were detected in children of mothers with GDM. Maternal body mass index was positively associated with children’s IgG MFI and number of IgG bands. Fewer IgA bands were detected in children with higher IgE levels, atopic dermatitis, asthma, and allergic rhinitis. More IgG bands were detected in children with higher anti-β-lactoglobulin IgG levels. Children with autoimmune risk-related HLA-DR3/DQ2.5 had fewer IgA bands, while those with neutral HLA-DR1/DQ5 had higher IgA, but lower IgG MFI. These results indicate that maternal prenatal changes could affect their offspring’s immune response against C. butyricum. Moreover, C. butyricum could have a protective role against allergic sensitization.
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spelling doaj-art-84188bc8dbda40a5a085f9641bfe11102025-08-20T03:08:48ZengTaylor & Francis GroupImmunological Medicine2578-58262025-05-0111110.1080/25785826.2025.2504021Antibodies against Clostridium butyricum in the children of mothers at risk for gestational diabetesCeleste Peterson0Aili Tagoma1Kristi Alnek2Anu Bärenson3Tamara Vorobjova4Ija Talja5Helis Janson6Anne Kirss7Siiri Kõljalg8Aki Sinkkonen9Marja Irmeli Roslund10Raivo Uibo11Department of Immunology, Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, EstoniaDepartment of Immunology, Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, EstoniaDepartment of Immunology, Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, EstoniaDepartment of Immunology, Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, EstoniaDepartment of Immunology, Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, EstoniaDepartment of Immunology, Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, EstoniaDepartment of Immunology, Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, EstoniaWomen’s Clinic, Tartu University Hospital, Tartu, EstoniaDepartment of Microbiology, Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, EstoniaHorticulture Technologies, Natural Resources Institute Finland, Turku, FinlandHorticulture Technologies, Natural Resources Institute Finland, Turku, FinlandDepartment of Immunology, Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, EstoniaGestational diabetes mellitus (GDM) is linked to an imbalance in gut microbiota composition, which can be transferred to the mother’s offspring. Clostridium butyricum, known for its health benefits in diabetes and allergy, lacks sufficient data regarding its effect on the immune system’s development in the offspring of mothers with GDM. This study assessed antibody responses against C. butyricum T2F3 in children of mothers at risk for GDM, involving 88 children aged 1–6 years. Antibody responses were measured with flow cytometry and immunoblot. Lower IgG median fluorescence intensity (MFI) values and fewer IgA and IgG bands against C. butyricum were detected in children of mothers with GDM. Maternal body mass index was positively associated with children’s IgG MFI and number of IgG bands. Fewer IgA bands were detected in children with higher IgE levels, atopic dermatitis, asthma, and allergic rhinitis. More IgG bands were detected in children with higher anti-β-lactoglobulin IgG levels. Children with autoimmune risk-related HLA-DR3/DQ2.5 had fewer IgA bands, while those with neutral HLA-DR1/DQ5 had higher IgA, but lower IgG MFI. These results indicate that maternal prenatal changes could affect their offspring’s immune response against C. butyricum. Moreover, C. butyricum could have a protective role against allergic sensitization.https://www.tandfonline.com/doi/10.1080/25785826.2025.2504021Clostridium butyricumgestational diabetes mellitusatopic dermatitisasthmaantibody
spellingShingle Celeste Peterson
Aili Tagoma
Kristi Alnek
Anu Bärenson
Tamara Vorobjova
Ija Talja
Helis Janson
Anne Kirss
Siiri Kõljalg
Aki Sinkkonen
Marja Irmeli Roslund
Raivo Uibo
Antibodies against Clostridium butyricum in the children of mothers at risk for gestational diabetes
Immunological Medicine
Clostridium butyricum
gestational diabetes mellitus
atopic dermatitis
asthma
antibody
title Antibodies against Clostridium butyricum in the children of mothers at risk for gestational diabetes
title_full Antibodies against Clostridium butyricum in the children of mothers at risk for gestational diabetes
title_fullStr Antibodies against Clostridium butyricum in the children of mothers at risk for gestational diabetes
title_full_unstemmed Antibodies against Clostridium butyricum in the children of mothers at risk for gestational diabetes
title_short Antibodies against Clostridium butyricum in the children of mothers at risk for gestational diabetes
title_sort antibodies against clostridium butyricum in the children of mothers at risk for gestational diabetes
topic Clostridium butyricum
gestational diabetes mellitus
atopic dermatitis
asthma
antibody
url https://www.tandfonline.com/doi/10.1080/25785826.2025.2504021
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