Identification of novel functional compounds from forest onion and its biological activities against breast cancer
The discovery of new molecules from natural sources for the treatment of tumors such as breast cancers is of importance for the development of functional foods and to the pharmaceutical industry. A natural resource with potential activity against breast cancer is forest onion, Eleutherine bulbosa (M...
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| Language: | English |
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Elsevier
2024-12-01
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| Series: | Journal of Agriculture and Food Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666154324003995 |
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| author | Fahrul Nurkolis Isma Kurniatanty Elvan Wiyarta Happy Kurnia Permatasari Nelly Mayulu Nurpudji Astuti Taslim Raymond Rubianto Tjandrawinata Hardinsyah Hardinsyah Trina Ekawati Tallei Apollinaire Tsopmo Son Radu Edwin Hadinata Bonglee Kim Rosy Iara Maciel Azambuja Ribeiro Rony Abdi Syahputra |
| author_facet | Fahrul Nurkolis Isma Kurniatanty Elvan Wiyarta Happy Kurnia Permatasari Nelly Mayulu Nurpudji Astuti Taslim Raymond Rubianto Tjandrawinata Hardinsyah Hardinsyah Trina Ekawati Tallei Apollinaire Tsopmo Son Radu Edwin Hadinata Bonglee Kim Rosy Iara Maciel Azambuja Ribeiro Rony Abdi Syahputra |
| author_sort | Fahrul Nurkolis |
| collection | DOAJ |
| description | The discovery of new molecules from natural sources for the treatment of tumors such as breast cancers is of importance for the development of functional foods and to the pharmaceutical industry. A natural resource with potential activity against breast cancer is forest onion, Eleutherine bulbosa (Mill.) Urb., but the identity of its active constituents and their mechanisms of action remain unexplored. Therefore, this study focuses on metabolite profiling, in silico or pharmacoinformatic activity and mechanisms, as well as advanced validation on in vitro cell lines. Ten compounds identified in E. bulbosa bulb ethanolic extract (EBE) showed cancer receptor and radical inhibitory activity via network pharmacology and molecular docking simulation. The most promising compound was avenasterol binding PARP-1, HER2, iNOS receptors with values of −11.26, −8.34, and −9.17 μg/mL, respectively. EBE and avenasterol had a smaller EC50 value, or higher potency, than the control antioxidant Trolox in radical scavenging tests with ABTS and DPPH. In line with the in silico study, EBE and avenasterol showed antiproliferative activity against human breast cancer MCF-7 with LD50 217.8 μg/mL, with relatively low cytotoxicity to normal MCF-10A cells (LD50 > 1000 μg/mL). The antiproliferative mechanism of EBE on MCF-7 was associated with downregulation of TGF-β, HER2, PI3K, and AKT which are known tumor activators. Significant (p < 0.05) upregulation of tumor suppressor gene miR-29a-3p in MCF-7 was observed after treatment with EBE in a dose-dependent manner. |
| format | Article |
| id | doaj-art-84182acedf474881a495c067e542b5ad |
| institution | OA Journals |
| issn | 2666-1543 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Agriculture and Food Research |
| spelling | doaj-art-84182acedf474881a495c067e542b5ad2025-08-20T01:56:41ZengElsevierJournal of Agriculture and Food Research2666-15432024-12-011810136210.1016/j.jafr.2024.101362Identification of novel functional compounds from forest onion and its biological activities against breast cancerFahrul Nurkolis0Isma Kurniatanty1Elvan Wiyarta2Happy Kurnia Permatasari3Nelly Mayulu4Nurpudji Astuti Taslim5Raymond Rubianto Tjandrawinata6Hardinsyah Hardinsyah7Trina Ekawati Tallei8Apollinaire Tsopmo9Son Radu10Edwin Hadinata11Bonglee Kim12Rosy Iara Maciel Azambuja Ribeiro13Rony Abdi Syahputra14Department of Biological Sciences, Faculty of Sciences and Technology, State Islamic University of Sunan Kalijaga (UIN Sunan Kalijaga), Yogyakarta, 55281, IndonesiaDepartment of Biological Sciences, Faculty of Sciences and Technology, State Islamic University of Sunan Kalijaga (UIN Sunan Kalijaga), Yogyakarta, 55281, IndonesiaDepartment of Neurology, Faculty of Medicine, Universitas Indonesia - Dr. Cipto Mangunkusumo National Hospital, Jakarta, 10430, IndonesiaDepartment of Biochemistry and Biomolecular, Faculty of Medicine, University of Brawijaya, Malang, 65145, IndonesiaDepartment of Nutrition, Faculty of Health Science, Muhammadiyah Manado University, Manado, 95249, IndonesiaDivision of Clinical Nutrition, Department of Nutrition, Faculty of Medicine, Hasanuddin University, Makassar, 90245, IndonesiaDepartment of Biotechnology, Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia, Jakarta, 12930, IndonesiaDivision of Applied Nutrition, Department of Community Nutrition, Faculty of Human Ecology, IPB University, West Java, Bogor, 16680, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sam Ratulangi, Manado, 95115, IndonesiaFood Science Program, Department of Chemistry, Institute of Biochemistry, Carleton University, 1125 Colonel by Drive, Ottawa, K1S 5B6, ON, CanadaDepartment of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, Selangor, UPM Serdang, 43400, MalaysiaInternal Medicine, Hermina Pasuruan Hospital, Pasuruan, 67181, East Java, IndonesiaDepartment of Pathology, College of Korean Medicine, Kyung Hee University, Seou, 02447, Republic of KoreaExperimental Pathology Laboratory, Midwest Campus, Federal University of São João del-Rei, Divinópolis, BrazilDepartment of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia; Corresponding author.The discovery of new molecules from natural sources for the treatment of tumors such as breast cancers is of importance for the development of functional foods and to the pharmaceutical industry. A natural resource with potential activity against breast cancer is forest onion, Eleutherine bulbosa (Mill.) Urb., but the identity of its active constituents and their mechanisms of action remain unexplored. Therefore, this study focuses on metabolite profiling, in silico or pharmacoinformatic activity and mechanisms, as well as advanced validation on in vitro cell lines. Ten compounds identified in E. bulbosa bulb ethanolic extract (EBE) showed cancer receptor and radical inhibitory activity via network pharmacology and molecular docking simulation. The most promising compound was avenasterol binding PARP-1, HER2, iNOS receptors with values of −11.26, −8.34, and −9.17 μg/mL, respectively. EBE and avenasterol had a smaller EC50 value, or higher potency, than the control antioxidant Trolox in radical scavenging tests with ABTS and DPPH. In line with the in silico study, EBE and avenasterol showed antiproliferative activity against human breast cancer MCF-7 with LD50 217.8 μg/mL, with relatively low cytotoxicity to normal MCF-10A cells (LD50 > 1000 μg/mL). The antiproliferative mechanism of EBE on MCF-7 was associated with downregulation of TGF-β, HER2, PI3K, and AKT which are known tumor activators. Significant (p < 0.05) upregulation of tumor suppressor gene miR-29a-3p in MCF-7 was observed after treatment with EBE in a dose-dependent manner.http://www.sciencedirect.com/science/article/pii/S2666154324003995AnticancerNatural productFunctional foodAvenasterolPARP1MCF-7 |
| spellingShingle | Fahrul Nurkolis Isma Kurniatanty Elvan Wiyarta Happy Kurnia Permatasari Nelly Mayulu Nurpudji Astuti Taslim Raymond Rubianto Tjandrawinata Hardinsyah Hardinsyah Trina Ekawati Tallei Apollinaire Tsopmo Son Radu Edwin Hadinata Bonglee Kim Rosy Iara Maciel Azambuja Ribeiro Rony Abdi Syahputra Identification of novel functional compounds from forest onion and its biological activities against breast cancer Journal of Agriculture and Food Research Anticancer Natural product Functional food Avenasterol PARP1 MCF-7 |
| title | Identification of novel functional compounds from forest onion and its biological activities against breast cancer |
| title_full | Identification of novel functional compounds from forest onion and its biological activities against breast cancer |
| title_fullStr | Identification of novel functional compounds from forest onion and its biological activities against breast cancer |
| title_full_unstemmed | Identification of novel functional compounds from forest onion and its biological activities against breast cancer |
| title_short | Identification of novel functional compounds from forest onion and its biological activities against breast cancer |
| title_sort | identification of novel functional compounds from forest onion and its biological activities against breast cancer |
| topic | Anticancer Natural product Functional food Avenasterol PARP1 MCF-7 |
| url | http://www.sciencedirect.com/science/article/pii/S2666154324003995 |
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