Ramadan fasting and exercise combination therapy: A novel approach for osteoporosis prevention in ovariectomized rats
BackgroundOsteoporosis is a chronic bone metabolic disease that affects millions of people worldwide, particularly the elderly and postmenopausal women. It is characterized by weakened bones, increasing the risk of fractures and leading to significant morbidity and mortality. The goal of the current...
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Frontiers Media S.A.
2024-10-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2024.1403772/full |
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| author | Tarfa Albrahim Raghad Alangry Raghad Alotaibi Leen Almandil Sara Alburikan Hisham S. Aloudah Mohammed Alahmed Mohammed Alahmed Mona Alonazi |
| author_facet | Tarfa Albrahim Raghad Alangry Raghad Alotaibi Leen Almandil Sara Alburikan Hisham S. Aloudah Mohammed Alahmed Mohammed Alahmed Mona Alonazi |
| author_sort | Tarfa Albrahim |
| collection | DOAJ |
| description | BackgroundOsteoporosis is a chronic bone metabolic disease that affects millions of people worldwide, particularly the elderly and postmenopausal women. It is characterized by weakened bones, increasing the risk of fractures and leading to significant morbidity and mortality. The goal of the current study is to examine the reported osteo-preservative effects of exercise and/or fasting in the Ramadan fasting model (RFM) in ovariectomized (OVX) rats.MethodsThe experimental intervention started 1 month following the ovariectomy procedure and consisted of five 15-min exercise sessions per week at 18–25 m/min and/or an approximately 13-h fast from sunrise to sunset (6:00 AM–19:00 PM). Serum bone metabolism biomarker levels were measured, and mineral concentrations in femoral ashed bones and digested serum were determined. Additionally, serum bone alkaline phosphatase (b-ALP), parathyroid hormone, osteocalcin, calcitonin, and vitamin D3 concentrations were measured using the competitive enzyme immunoassay technique.ResultsCalcium, magnesium, and phosphorus showed a notable decrease in mineral concentration among OVX rat femurs compared with the combination group (OVX + RFM + E) and control groups. In addition, homeostasis of serum concentrations of calcium, magnesium, and phosphorus was observed to increase in the OVX + RFM + E group rather than in the OVX group without intervention when compared with a control group. Furthermore, fasting and exercise, either alone or concurrently with ovariectomy, induced a non-significant elevation in osteocalcin, parathyroid hormone, and vitamin D3, whereas b-ALP and calcitonin increased significantly compared with those in control rats.ConclusionThe combination of the Ramadan fasting model and moderate intensity exercises among OVX rats manifested advantageous effects in bone biomarkers compared with OVX rats without intervention. This could be recommended as a lifestyle modification that is protective against osteoporosis, especially in the context of depleted estrogen hormone after menopause. |
| format | Article |
| id | doaj-art-8411a2447d7b4cedb3b5a50eb8035c6a |
| institution | OA Journals |
| issn | 1664-042X |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj-art-8411a2447d7b4cedb3b5a50eb8035c6a2025-08-20T02:09:55ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2024-10-011510.3389/fphys.2024.14037721403772Ramadan fasting and exercise combination therapy: A novel approach for osteoporosis prevention in ovariectomized ratsTarfa Albrahim0Raghad Alangry1Raghad Alotaibi2Leen Almandil3Sara Alburikan4Hisham S. Aloudah5Mohammed Alahmed6Mohammed Alahmed7Mona Alonazi8Department of Health Sciences, Clinical Nutrition, College of Health and Rehabilitation Sciences, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi ArabiaDepartment of Health Sciences, Clinical Nutrition, College of Health and Rehabilitation Sciences, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi ArabiaDepartment of Health Sciences, Clinical Nutrition, College of Health and Rehabilitation Sciences, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi ArabiaDepartment of Health Sciences, Clinical Nutrition, College of Health and Rehabilitation Sciences, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi ArabiaDepartment of Health Sciences, Clinical Nutrition, College of Health and Rehabilitation Sciences, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi ArabiaPrince Naif for Health Research Center, King Saud University, Riyadh, Saudi ArabiaPrince Naif for Health Research Center, King Saud University, Riyadh, Saudi ArabiaResearch Office, Johns Hopkins Aramco Healthcare, Dhahran, Saudi ArabiaDepartment of Biochemistry, College of Science, King Saud University, Riyadh, Saudi ArabiaBackgroundOsteoporosis is a chronic bone metabolic disease that affects millions of people worldwide, particularly the elderly and postmenopausal women. It is characterized by weakened bones, increasing the risk of fractures and leading to significant morbidity and mortality. The goal of the current study is to examine the reported osteo-preservative effects of exercise and/or fasting in the Ramadan fasting model (RFM) in ovariectomized (OVX) rats.MethodsThe experimental intervention started 1 month following the ovariectomy procedure and consisted of five 15-min exercise sessions per week at 18–25 m/min and/or an approximately 13-h fast from sunrise to sunset (6:00 AM–19:00 PM). Serum bone metabolism biomarker levels were measured, and mineral concentrations in femoral ashed bones and digested serum were determined. Additionally, serum bone alkaline phosphatase (b-ALP), parathyroid hormone, osteocalcin, calcitonin, and vitamin D3 concentrations were measured using the competitive enzyme immunoassay technique.ResultsCalcium, magnesium, and phosphorus showed a notable decrease in mineral concentration among OVX rat femurs compared with the combination group (OVX + RFM + E) and control groups. In addition, homeostasis of serum concentrations of calcium, magnesium, and phosphorus was observed to increase in the OVX + RFM + E group rather than in the OVX group without intervention when compared with a control group. Furthermore, fasting and exercise, either alone or concurrently with ovariectomy, induced a non-significant elevation in osteocalcin, parathyroid hormone, and vitamin D3, whereas b-ALP and calcitonin increased significantly compared with those in control rats.ConclusionThe combination of the Ramadan fasting model and moderate intensity exercises among OVX rats manifested advantageous effects in bone biomarkers compared with OVX rats without intervention. This could be recommended as a lifestyle modification that is protective against osteoporosis, especially in the context of depleted estrogen hormone after menopause.https://www.frontiersin.org/articles/10.3389/fphys.2024.1403772/fullovariectomyRamadan fastingexercisebone metabolismestrogen deficiency |
| spellingShingle | Tarfa Albrahim Raghad Alangry Raghad Alotaibi Leen Almandil Sara Alburikan Hisham S. Aloudah Mohammed Alahmed Mohammed Alahmed Mona Alonazi Ramadan fasting and exercise combination therapy: A novel approach for osteoporosis prevention in ovariectomized rats Frontiers in Physiology ovariectomy Ramadan fasting exercise bone metabolism estrogen deficiency |
| title | Ramadan fasting and exercise combination therapy: A novel approach for osteoporosis prevention in ovariectomized rats |
| title_full | Ramadan fasting and exercise combination therapy: A novel approach for osteoporosis prevention in ovariectomized rats |
| title_fullStr | Ramadan fasting and exercise combination therapy: A novel approach for osteoporosis prevention in ovariectomized rats |
| title_full_unstemmed | Ramadan fasting and exercise combination therapy: A novel approach for osteoporosis prevention in ovariectomized rats |
| title_short | Ramadan fasting and exercise combination therapy: A novel approach for osteoporosis prevention in ovariectomized rats |
| title_sort | ramadan fasting and exercise combination therapy a novel approach for osteoporosis prevention in ovariectomized rats |
| topic | ovariectomy Ramadan fasting exercise bone metabolism estrogen deficiency |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2024.1403772/full |
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