Comprehensive analysis of antimicrobial resistance dynamics among broiler and duck intensive production systems
Abstract Antimicrobial resistance (AMR) is a global health challenge requiring cross-sector action, with research largely focused on chickens, leaving ducks underexplored. This study examines AMR dynamics in Ross 308 broilers and Cherry Valley ducks over 15 months and 15 stocking periods under consi...
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Nature Portfolio
2025-02-01
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| Online Access: | https://doi.org/10.1038/s41598-025-89432-z |
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| author | Zsombor Szoke Peter Fauszt Maja Mikolas Peter David Emese Szilagyi-Tolnai Georgina Pesti-Asboth Judit Rita Homoki Ildiko Kovacs-Forgacs Ferenc Gal Laszlo Stundl Levente Czegledi Aniko Stagel Sandor Biro Judit Remenyik Melinda Paholcsek |
| author_facet | Zsombor Szoke Peter Fauszt Maja Mikolas Peter David Emese Szilagyi-Tolnai Georgina Pesti-Asboth Judit Rita Homoki Ildiko Kovacs-Forgacs Ferenc Gal Laszlo Stundl Levente Czegledi Aniko Stagel Sandor Biro Judit Remenyik Melinda Paholcsek |
| author_sort | Zsombor Szoke |
| collection | DOAJ |
| description | Abstract Antimicrobial resistance (AMR) is a global health challenge requiring cross-sector action, with research largely focused on chickens, leaving ducks underexplored. This study examines AMR dynamics in Ross 308 broilers and Cherry Valley ducks over 15 months and 15 stocking periods under consistent rearing conditions. A total of 96 pooled samples were collected: 50 from broiler farms (26 biological, 24 environmental) and 46 from duck farms (24 biological, 22 environmental). Using next-generation shotgun sequencing, 3,665 distinct AMR types were identified: 1,918 in broilers and 1,747 in ducks. Host-specific AMRs comprised 25.3% in broilers and 18% in ducks, while 56.7% were shared. AMR diversity declined across production phases, with broilers losing 641 types and ducks losing 308, yet AMR frequencies increased significantly by the finisher phase (p < 0.0001). Based on in silico data, prophylactic antibiotic use significantly reduced the prevalence of multidrug-resistant bacteria in both poultry species (p < 0.05). Hospital-acquired infection-associated AMRs were higher in broilers than in ducks at the start of production but declined significantly by the end of the rearing period (p < 0.0001). Above-average resistance markers accounted for approximately 10% of all detected resistance determinants. Tetracycline and phenicol resistances emerged as the most prevalent. 13 high-resistance carrier (HRC) species were shared between both hosts. Broiler-specific HRCs exhibited significantly higher abundances (relative frequency: 0.08) than duck-specific HRCs (relative frequency: 0.003, p = 0.035). The grower phase emerged as a critical intervention point. In farm environments 15 broiler-specific and 9 duck-specific biomarker species were identified, each strongly correlated with poultry-core HRCs (correlation coefficient > 0.7). Broiler exhibited higher abundances of key resistance genes, with tetracycline resistance predominantly associated with Bacteroides coprosuis, Pasteurella multocida, and Acinetobacter baumannii. Despite its limitations, this research provides key insights into AMR trends in two major poultry types, guiding targeted interventions and sustainable management strategies. |
| format | Article |
| id | doaj-art-840b7a19876649d6a0651c788a02564b |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-02-01 |
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| spelling | doaj-art-840b7a19876649d6a0651c788a02564b2025-02-09T12:36:23ZengNature PortfolioScientific Reports2045-23222025-02-0115112310.1038/s41598-025-89432-zComprehensive analysis of antimicrobial resistance dynamics among broiler and duck intensive production systemsZsombor Szoke0Peter Fauszt1Maja Mikolas2Peter David3Emese Szilagyi-Tolnai4Georgina Pesti-Asboth5Judit Rita Homoki6Ildiko Kovacs-Forgacs7Ferenc Gal8Laszlo Stundl9Levente Czegledi10Aniko Stagel11Sandor Biro12Judit Remenyik13Melinda Paholcsek14Faculty of Agricultural and Food Sciences and Environmental Management, Complex Systems and Microbiome-innovations Centre, University of DebrecenFaculty of Agricultural and Food Sciences and Environmental Management, Complex Systems and Microbiome-innovations Centre, University of DebrecenFaculty of Agricultural and Food Sciences and Environmental Management, Complex Systems and Microbiome-innovations Centre, University of DebrecenFaculty of Agricultural and Food Sciences and Environmental Management, Complex Systems and Microbiome-innovations Centre, University of DebrecenFaculty of Agricultural and Food Sciences and Environmental Management, Complex Systems and Microbiome-innovations Centre, University of DebrecenFaculty of Agricultural and Food Sciences and Environmental Management, Complex Systems and Microbiome-innovations Centre, University of DebrecenFaculty of Agricultural and Food Sciences and Environmental Management, Complex Systems and Microbiome-innovations Centre, University of DebrecenFaculty of Agricultural and Food Sciences and Environmental Management, Complex Systems and Microbiome-innovations Centre, University of DebrecenFaculty of Agricultural and Food Sciences and Environmental Management, Complex Systems and Microbiome-innovations Centre, University of DebrecenFaculty of Agricultural and Food Sciences and Environmental Management, University of DebrecenFaculty of Agricultural and Food Sciences and Environmental Management, Institute of Animal Science, Biotechnology and Nature Conservation, Department of Animal Husbandry, University of DebrecenHungarian National Blood Transfusion Service Nucleic Acid Testing LaboratoryFaculty of Medicine, Department of Human Genetics, University of DebrecenFaculty of Agricultural and Food Sciences and Environmental Management, Complex Systems and Microbiome-innovations Centre, University of DebrecenFaculty of Agricultural and Food Sciences and Environmental Management, Complex Systems and Microbiome-innovations Centre, University of DebrecenAbstract Antimicrobial resistance (AMR) is a global health challenge requiring cross-sector action, with research largely focused on chickens, leaving ducks underexplored. This study examines AMR dynamics in Ross 308 broilers and Cherry Valley ducks over 15 months and 15 stocking periods under consistent rearing conditions. A total of 96 pooled samples were collected: 50 from broiler farms (26 biological, 24 environmental) and 46 from duck farms (24 biological, 22 environmental). Using next-generation shotgun sequencing, 3,665 distinct AMR types were identified: 1,918 in broilers and 1,747 in ducks. Host-specific AMRs comprised 25.3% in broilers and 18% in ducks, while 56.7% were shared. AMR diversity declined across production phases, with broilers losing 641 types and ducks losing 308, yet AMR frequencies increased significantly by the finisher phase (p < 0.0001). Based on in silico data, prophylactic antibiotic use significantly reduced the prevalence of multidrug-resistant bacteria in both poultry species (p < 0.05). Hospital-acquired infection-associated AMRs were higher in broilers than in ducks at the start of production but declined significantly by the end of the rearing period (p < 0.0001). Above-average resistance markers accounted for approximately 10% of all detected resistance determinants. Tetracycline and phenicol resistances emerged as the most prevalent. 13 high-resistance carrier (HRC) species were shared between both hosts. Broiler-specific HRCs exhibited significantly higher abundances (relative frequency: 0.08) than duck-specific HRCs (relative frequency: 0.003, p = 0.035). The grower phase emerged as a critical intervention point. In farm environments 15 broiler-specific and 9 duck-specific biomarker species were identified, each strongly correlated with poultry-core HRCs (correlation coefficient > 0.7). Broiler exhibited higher abundances of key resistance genes, with tetracycline resistance predominantly associated with Bacteroides coprosuis, Pasteurella multocida, and Acinetobacter baumannii. Despite its limitations, this research provides key insights into AMR trends in two major poultry types, guiding targeted interventions and sustainable management strategies.https://doi.org/10.1038/s41598-025-89432-zPoultry resistomePoultry microbiomeProphylactic antibiotic useIntensive broiler rearingIntensive duck rearingBiomarkers |
| spellingShingle | Zsombor Szoke Peter Fauszt Maja Mikolas Peter David Emese Szilagyi-Tolnai Georgina Pesti-Asboth Judit Rita Homoki Ildiko Kovacs-Forgacs Ferenc Gal Laszlo Stundl Levente Czegledi Aniko Stagel Sandor Biro Judit Remenyik Melinda Paholcsek Comprehensive analysis of antimicrobial resistance dynamics among broiler and duck intensive production systems Scientific Reports Poultry resistome Poultry microbiome Prophylactic antibiotic use Intensive broiler rearing Intensive duck rearing Biomarkers |
| title | Comprehensive analysis of antimicrobial resistance dynamics among broiler and duck intensive production systems |
| title_full | Comprehensive analysis of antimicrobial resistance dynamics among broiler and duck intensive production systems |
| title_fullStr | Comprehensive analysis of antimicrobial resistance dynamics among broiler and duck intensive production systems |
| title_full_unstemmed | Comprehensive analysis of antimicrobial resistance dynamics among broiler and duck intensive production systems |
| title_short | Comprehensive analysis of antimicrobial resistance dynamics among broiler and duck intensive production systems |
| title_sort | comprehensive analysis of antimicrobial resistance dynamics among broiler and duck intensive production systems |
| topic | Poultry resistome Poultry microbiome Prophylactic antibiotic use Intensive broiler rearing Intensive duck rearing Biomarkers |
| url | https://doi.org/10.1038/s41598-025-89432-z |
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