In ovo toxico-pathological effects of medicinal plants used against coccidiosis on chicken embryos development and hatchability
TOXICO-pathological effects of herbal plants have always been a major concern, but little information is provided on the toxico-pathological effects of medicinal plants used against coccidiosis. This study aimed to assess the histopathological effects of Carica papaya seeds (CPS), Azadirachta indica...
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| Language: | English |
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Elsevier
2024-12-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124010137 |
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| author | Ferdinand G. Tchodo Hervé B. Dakpogan Benjamin Adjei-Mensah Ombortime N'nanle Simplice Karou Wéré Pitala Kokou Tona Batomayena Bakoma |
| author_facet | Ferdinand G. Tchodo Hervé B. Dakpogan Benjamin Adjei-Mensah Ombortime N'nanle Simplice Karou Wéré Pitala Kokou Tona Batomayena Bakoma |
| author_sort | Ferdinand G. Tchodo |
| collection | DOAJ |
| description | TOXICO-pathological effects of herbal plants have always been a major concern, but little information is provided on the toxico-pathological effects of medicinal plants used against coccidiosis. This study aimed to assess the histopathological effects of Carica papaya seeds (CPS), Azadirachta indica leaves (AIL), and Sarcocephalus latifolius root (SLR) used as coccidiostat in traditional poultry farms with various doses using the chick embryo model. A total of 420 Sasso breeder eggs at ED4 of incubation were inoculated with the extract of these plants following a 3×4 factorial design with 3 plant extracts (CPS, AIL and SLR) and 4 inoculation doses (0, 25, 50 and 100 mg/kg egg-weight). From ED6 to ED19, the weights of the albumen and embryo were recorded, and the weights of ED19 embryo organs such as liver, heart, kidney, and lungs were measured. Additionally, histopathological lesions were examined. The results indicated that the presence of various phytoconstituents such as alkaloids, phenolics, flavonoids, tannins, saponins, coumarins, steroids, and triterpenes with statistically significant free-radical-scavenging ability differed among the plant extracts (p < 0.0001). Toxico-pathological examination revealed a dose-dependent slight toxicity (p < 0.0001) of the Azadirachta indica leaves extract compared to the other plants. Additionally, the relative organ weight showed kidney hypertrophy (p = 0.001) and liver hypertrophy (p = 0.0001), as well as dilation of hepatic and cardiac vessels. The conclusion drawn was that chicken embryos are more susceptible to in ovo inoculation with Azadirachta indica leaves compared to Carica papaya seeds and Sarcocephalus latifolius root. |
| format | Article |
| id | doaj-art-840043d2b00e47c5b41271d2c367bab4 |
| institution | OA Journals |
| issn | 0032-5791 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-840043d2b00e47c5b41271d2c367bab42025-08-20T02:37:45ZengElsevierPoultry Science0032-57912024-12-011031210443510.1016/j.psj.2024.104435In ovo toxico-pathological effects of medicinal plants used against coccidiosis on chicken embryos development and hatchabilityFerdinand G. Tchodo0Hervé B. Dakpogan1Benjamin Adjei-Mensah2Ombortime N'nanle3Simplice Karou4Wéré Pitala5Kokou Tona6Batomayena Bakoma7Regional Center of Excellence in Poultry Science, University of Lome, BSP 1515 Lome, Togo; Corresponding author.School of Animal Production System Management P.O.BOX 43 Ketou, National Agricultural University, 01 P.O.BOX 55 Porto-Novo, BeninRegional Center of Excellence in Poultry Science, University of Lome, BSP 1515 Lome, TogoRegional Center of Excellence in Poultry Science, University of Lome, BSP 1515 Lome, TogoRegional Center of Excellence in Poultry Science, University of Lome, BSP 1515 Lome, TogoRegional Center of Excellence in Poultry Science, University of Lome, BSP 1515 Lome, TogoRegional Center of Excellence in Poultry Science, University of Lome, BSP 1515 Lome, TogoPharmaceutical Sciences Research Laboratory, University of Lome, BSP 1515 Lome, TogoTOXICO-pathological effects of herbal plants have always been a major concern, but little information is provided on the toxico-pathological effects of medicinal plants used against coccidiosis. This study aimed to assess the histopathological effects of Carica papaya seeds (CPS), Azadirachta indica leaves (AIL), and Sarcocephalus latifolius root (SLR) used as coccidiostat in traditional poultry farms with various doses using the chick embryo model. A total of 420 Sasso breeder eggs at ED4 of incubation were inoculated with the extract of these plants following a 3×4 factorial design with 3 plant extracts (CPS, AIL and SLR) and 4 inoculation doses (0, 25, 50 and 100 mg/kg egg-weight). From ED6 to ED19, the weights of the albumen and embryo were recorded, and the weights of ED19 embryo organs such as liver, heart, kidney, and lungs were measured. Additionally, histopathological lesions were examined. The results indicated that the presence of various phytoconstituents such as alkaloids, phenolics, flavonoids, tannins, saponins, coumarins, steroids, and triterpenes with statistically significant free-radical-scavenging ability differed among the plant extracts (p < 0.0001). Toxico-pathological examination revealed a dose-dependent slight toxicity (p < 0.0001) of the Azadirachta indica leaves extract compared to the other plants. Additionally, the relative organ weight showed kidney hypertrophy (p = 0.001) and liver hypertrophy (p = 0.0001), as well as dilation of hepatic and cardiac vessels. The conclusion drawn was that chicken embryos are more susceptible to in ovo inoculation with Azadirachta indica leaves compared to Carica papaya seeds and Sarcocephalus latifolius root.http://www.sciencedirect.com/science/article/pii/S0032579124010137Toxico-pathologyAntioxidantMedicinal plantCoccidiosisEmbryonic development |
| spellingShingle | Ferdinand G. Tchodo Hervé B. Dakpogan Benjamin Adjei-Mensah Ombortime N'nanle Simplice Karou Wéré Pitala Kokou Tona Batomayena Bakoma In ovo toxico-pathological effects of medicinal plants used against coccidiosis on chicken embryos development and hatchability Poultry Science Toxico-pathology Antioxidant Medicinal plant Coccidiosis Embryonic development |
| title | In ovo toxico-pathological effects of medicinal plants used against coccidiosis on chicken embryos development and hatchability |
| title_full | In ovo toxico-pathological effects of medicinal plants used against coccidiosis on chicken embryos development and hatchability |
| title_fullStr | In ovo toxico-pathological effects of medicinal plants used against coccidiosis on chicken embryos development and hatchability |
| title_full_unstemmed | In ovo toxico-pathological effects of medicinal plants used against coccidiosis on chicken embryos development and hatchability |
| title_short | In ovo toxico-pathological effects of medicinal plants used against coccidiosis on chicken embryos development and hatchability |
| title_sort | in ovo toxico pathological effects of medicinal plants used against coccidiosis on chicken embryos development and hatchability |
| topic | Toxico-pathology Antioxidant Medicinal plant Coccidiosis Embryonic development |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124010137 |
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