Biological interaction of bioactive polymeric membranes in induced bone defects in rabbit tibias.

The study aimed to evaluate bone repair using three osteoinductive polymers in bone defects created in rabbit tibias. Forty-eight adult rabbits were assessed at various time points: three, seven, fourteen, and thirty days. The groups included a control group (without biomaterial), M1 (Poly L Lactide...

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Main Authors: Olicies da Cunha, Cássio Ricardo Auada Ferrigno, Solimar Dutra da Silveira, Bruno Gregnanin Pedron, Danielle Tiemi Komorizono, Flávia Cristina Rosin Prado, Walter Israel Rojas Cabrera, Luciana Corrêa
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2024-01-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0313834&type=printable
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author Olicies da Cunha
Cássio Ricardo Auada Ferrigno
Solimar Dutra da Silveira
Bruno Gregnanin Pedron
Danielle Tiemi Komorizono
Flávia Cristina Rosin Prado
Walter Israel Rojas Cabrera
Luciana Corrêa
author_facet Olicies da Cunha
Cássio Ricardo Auada Ferrigno
Solimar Dutra da Silveira
Bruno Gregnanin Pedron
Danielle Tiemi Komorizono
Flávia Cristina Rosin Prado
Walter Israel Rojas Cabrera
Luciana Corrêa
author_sort Olicies da Cunha
collection DOAJ
description The study aimed to evaluate bone repair using three osteoinductive polymers in bone defects created in rabbit tibias. Forty-eight adult rabbits were assessed at various time points: three, seven, fourteen, and thirty days. The groups included a control group (without biomaterial), M1 (Poly L Lactide co Polycaprolactone/Polyethylene Glycol), M2 (Poly L Lactide co Polycaprolactone/Polyethylene Glycol/β-Tricalcium Phosphate), and M3 (Poly L Lactide co Polycaprolactone/Polyethylene Glycol/nano hydroxyapatite). Histomorphometric analysis was conducted to evaluate new bone formation within and around the bone defect. At 14 (p<0.05) and 30 days (p<0.05), the callus area in the membrane groups, particularly in M3, was also significantly larger than in the control group, indicating the osteoinductive potential of these biomaterials. The callus consisted of both bone and cartilaginous matrix, suggesting a robust activation of endochondral ossification. The number of osteoclast was higher in the membrane groups, especially at 14 days in the M3 group, indicating increased bone remodeling activity. The membranes were not fully absorbed by 30 days, creating a space between the defect and the periosteum. In conclusion, all three membranes showed significant chondro and osteoinductive potential, with the membrane containing nano-hydroxyapatite demonstrating the most pronounced potential.
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spelling doaj-art-83fcf41c1e204fc19e2660d2a0ac04de2025-08-20T01:59:21ZengPublic Library of Science (PLoS)PLoS ONE1932-62032024-01-011912e031383410.1371/journal.pone.0313834Biological interaction of bioactive polymeric membranes in induced bone defects in rabbit tibias.Olicies da CunhaCássio Ricardo Auada FerrignoSolimar Dutra da SilveiraBruno Gregnanin PedronDanielle Tiemi KomorizonoFlávia Cristina Rosin PradoWalter Israel Rojas CabreraLuciana CorrêaThe study aimed to evaluate bone repair using three osteoinductive polymers in bone defects created in rabbit tibias. Forty-eight adult rabbits were assessed at various time points: three, seven, fourteen, and thirty days. The groups included a control group (without biomaterial), M1 (Poly L Lactide co Polycaprolactone/Polyethylene Glycol), M2 (Poly L Lactide co Polycaprolactone/Polyethylene Glycol/β-Tricalcium Phosphate), and M3 (Poly L Lactide co Polycaprolactone/Polyethylene Glycol/nano hydroxyapatite). Histomorphometric analysis was conducted to evaluate new bone formation within and around the bone defect. At 14 (p<0.05) and 30 days (p<0.05), the callus area in the membrane groups, particularly in M3, was also significantly larger than in the control group, indicating the osteoinductive potential of these biomaterials. The callus consisted of both bone and cartilaginous matrix, suggesting a robust activation of endochondral ossification. The number of osteoclast was higher in the membrane groups, especially at 14 days in the M3 group, indicating increased bone remodeling activity. The membranes were not fully absorbed by 30 days, creating a space between the defect and the periosteum. In conclusion, all three membranes showed significant chondro and osteoinductive potential, with the membrane containing nano-hydroxyapatite demonstrating the most pronounced potential.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0313834&type=printable
spellingShingle Olicies da Cunha
Cássio Ricardo Auada Ferrigno
Solimar Dutra da Silveira
Bruno Gregnanin Pedron
Danielle Tiemi Komorizono
Flávia Cristina Rosin Prado
Walter Israel Rojas Cabrera
Luciana Corrêa
Biological interaction of bioactive polymeric membranes in induced bone defects in rabbit tibias.
PLoS ONE
title Biological interaction of bioactive polymeric membranes in induced bone defects in rabbit tibias.
title_full Biological interaction of bioactive polymeric membranes in induced bone defects in rabbit tibias.
title_fullStr Biological interaction of bioactive polymeric membranes in induced bone defects in rabbit tibias.
title_full_unstemmed Biological interaction of bioactive polymeric membranes in induced bone defects in rabbit tibias.
title_short Biological interaction of bioactive polymeric membranes in induced bone defects in rabbit tibias.
title_sort biological interaction of bioactive polymeric membranes in induced bone defects in rabbit tibias
url https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0313834&type=printable
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