Ethylene Response Factor Sl-ERF.B.3 Is Responsive to Abiotic Stresses and Mediates Salt and Cold Stress Response Regulation in Tomato
Sl-ERF.B.3 (Solanum lycopersicum ethylene response factor B.3) gene encodes for a tomato transcription factor of the ERF (ethylene responsive factor) family. Our results of real-time RT-PCR showed that Sl-ERF.B.3 is an abiotic stress responsive gene, which is induced by cold, heat, and flooding, but...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/167681 |
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| author | Imen Klay Julien Pirrello Leila Riahi Anne Bernadac Ameur Cherif Mondher Bouzayen Sadok Bouzid |
| author_facet | Imen Klay Julien Pirrello Leila Riahi Anne Bernadac Ameur Cherif Mondher Bouzayen Sadok Bouzid |
| author_sort | Imen Klay |
| collection | DOAJ |
| description | Sl-ERF.B.3 (Solanum lycopersicum ethylene response factor B.3) gene encodes for a tomato transcription factor of the ERF (ethylene responsive factor) family. Our results of real-time RT-PCR showed that Sl-ERF.B.3 is an abiotic stress responsive gene, which is induced by cold, heat, and flooding, but downregulated by salinity and drought. To get more insight into the role of Sl-ERF.B.3 in plant response to separate salinity and cold, a comparative study between wild type and two Sl-ERF.B.3 antisense transgenic tomato lines was achieved. Compared with wild type, Sl-ERF.B.3 antisense transgenic plants exhibited a salt stress dependent growth inhibition. This inhibition was significantly enhanced in shoots but reduced in roots, leading to an increased root to shoot ratio. Furthermore, the cold stress essay clearly revealed that introducing antisense Sl-ERF.B.3 in transgenic tomato plants reduces their cell injury and enhances their tolerance against 14 d of cold stress. All these results suggest that Sl-ERF.B.3 gene is involved in plant response to abiotic stresses and may play a role in the layout of stress symptoms under cold stress and in growth regulation under salinity. |
| format | Article |
| id | doaj-art-83e933c672cd48d2b822f9c47c98c968 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-83e933c672cd48d2b822f9c47c98c9682025-02-03T05:52:08ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/167681167681Ethylene Response Factor Sl-ERF.B.3 Is Responsive to Abiotic Stresses and Mediates Salt and Cold Stress Response Regulation in TomatoImen Klay0Julien Pirrello1Leila Riahi2Anne Bernadac3Ameur Cherif4Mondher Bouzayen5Sadok Bouzid6Laboratoire de Morphogenèse et de Biotechnologie Végétale, Faculté des Sciences de Tunis (FST), Campus Universitaire 2092 El Manar Tunis, TunisiaUniversité Toulouse, INP ENSA Toulouse, 31326 Castanet-Tolosan, FranceLR Biotechnologie et Valorisation des Bio-Géo Ressources (LR11ES31), Institut Supérieur de Biotechnologie, Université de La Manouba Biotech Pole de Sidi Thabet, Sidi Thabet, 2020 Ariana, TunisiaUniversité Toulouse, INP ENSA Toulouse, 31326 Castanet-Tolosan, FranceLR Biotechnologie et Valorisation des Bio-Géo Ressources (LR11ES31), Institut Supérieur de Biotechnologie, Université de La Manouba Biotech Pole de Sidi Thabet, Sidi Thabet, 2020 Ariana, TunisiaUniversité Toulouse, INP ENSA Toulouse, 31326 Castanet-Tolosan, FranceLaboratoire de Morphogenèse et de Biotechnologie Végétale, Faculté des Sciences de Tunis (FST), Campus Universitaire 2092 El Manar Tunis, TunisiaSl-ERF.B.3 (Solanum lycopersicum ethylene response factor B.3) gene encodes for a tomato transcription factor of the ERF (ethylene responsive factor) family. Our results of real-time RT-PCR showed that Sl-ERF.B.3 is an abiotic stress responsive gene, which is induced by cold, heat, and flooding, but downregulated by salinity and drought. To get more insight into the role of Sl-ERF.B.3 in plant response to separate salinity and cold, a comparative study between wild type and two Sl-ERF.B.3 antisense transgenic tomato lines was achieved. Compared with wild type, Sl-ERF.B.3 antisense transgenic plants exhibited a salt stress dependent growth inhibition. This inhibition was significantly enhanced in shoots but reduced in roots, leading to an increased root to shoot ratio. Furthermore, the cold stress essay clearly revealed that introducing antisense Sl-ERF.B.3 in transgenic tomato plants reduces their cell injury and enhances their tolerance against 14 d of cold stress. All these results suggest that Sl-ERF.B.3 gene is involved in plant response to abiotic stresses and may play a role in the layout of stress symptoms under cold stress and in growth regulation under salinity.http://dx.doi.org/10.1155/2014/167681 |
| spellingShingle | Imen Klay Julien Pirrello Leila Riahi Anne Bernadac Ameur Cherif Mondher Bouzayen Sadok Bouzid Ethylene Response Factor Sl-ERF.B.3 Is Responsive to Abiotic Stresses and Mediates Salt and Cold Stress Response Regulation in Tomato The Scientific World Journal |
| title | Ethylene Response Factor Sl-ERF.B.3 Is Responsive to Abiotic Stresses and Mediates Salt and Cold Stress Response Regulation in Tomato |
| title_full | Ethylene Response Factor Sl-ERF.B.3 Is Responsive to Abiotic Stresses and Mediates Salt and Cold Stress Response Regulation in Tomato |
| title_fullStr | Ethylene Response Factor Sl-ERF.B.3 Is Responsive to Abiotic Stresses and Mediates Salt and Cold Stress Response Regulation in Tomato |
| title_full_unstemmed | Ethylene Response Factor Sl-ERF.B.3 Is Responsive to Abiotic Stresses and Mediates Salt and Cold Stress Response Regulation in Tomato |
| title_short | Ethylene Response Factor Sl-ERF.B.3 Is Responsive to Abiotic Stresses and Mediates Salt and Cold Stress Response Regulation in Tomato |
| title_sort | ethylene response factor sl erf b 3 is responsive to abiotic stresses and mediates salt and cold stress response regulation in tomato |
| url | http://dx.doi.org/10.1155/2014/167681 |
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