Effect of Different Irrigating Solutions on the Microhardness of Dentin
Background: The selection of irrigating solutions during root canal therapy is essential for maintaining dentin structure and ensuring effective disinfection. However, various irrigants can alter the microhardness of dentin, which might have an impact on the treated tooth’s mechanical characteristic...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2025-06-01
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| Series: | Journal of Pharmacy and Bioallied Sciences |
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| Online Access: | https://journals.lww.com/10.4103/jpbs.jpbs_1653_24 |
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| author | Saron Ramesh Nair Mustafa Hussein Alattas Luluah Khalid Alhagas John Kodumbilayiparambil Anto Nasarudheen Cholayil Abdul Sathar Malayil |
| author_facet | Saron Ramesh Nair Mustafa Hussein Alattas Luluah Khalid Alhagas John Kodumbilayiparambil Anto Nasarudheen Cholayil Abdul Sathar Malayil |
| author_sort | Saron Ramesh Nair |
| collection | DOAJ |
| description | Background:
The selection of irrigating solutions during root canal therapy is essential for maintaining dentin structure and ensuring effective disinfection. However, various irrigants can alter the microhardness of dentin, which might have an impact on the treated tooth’s mechanical characteristics and long-term viability.
Materials and Methods:
To produce uniform dentin discs from the central portion of the root, forty human premolars were sectioned. Based on the irrigating solution employed, the specimens were split into four groups (n = 10): Group 1 received 2.5% sodium hypochlorite (NaOCl), Group 2 received 17% ethylenediaminetetraacetic acid (EDTA), Group 3 received 2% chlorhexidine (CHX), and Group 4 received distilled water (control). Each specimen was submerged in its respective irrigant for five minutes. A Vickers microhardness tester was used to determine microhardness at 50 g force for 10 seconds both before and after irrigation.
Results:
Microhardness decreased significantly in Group 1 (NaOCl), with a mean drop of 18% (from 55 ± 2.3 VHN to 45 ± 1.9 VHN). The greatest reduction, 28%, was observed in Group 2 (EDTA) (from 53 ± 1.8 VHN to 38 ± 2.1 VHN). The microhardness of Group 3 (CHX) decreased by only 5% (from 54 ± 2.0 VHN to 51 ± 1.5 VHN). Microhardness showed no significant change in the control group (Group 4). Significant differences between the groups were found by statistical analysis (ANOVA, P < 0.05), with Group 2 exhibiting the greatest influence on dentin microhardness.
Conclusion:
Dentin microhardness was most significantly reduced by EDTA, followed by NaOCl, with CHX showing only a minor effect. These results imply that frequent or extended usage of EDTA and NaOCl may cause the dentin structure to deteriorate. |
| format | Article |
| id | doaj-art-83dcfb1d66044e07969294042413be39 |
| institution | DOAJ |
| issn | 0976-4879 0975-7406 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Pharmacy and Bioallied Sciences |
| spelling | doaj-art-83dcfb1d66044e07969294042413be392025-08-20T03:15:20ZengWolters Kluwer Medknow PublicationsJournal of Pharmacy and Bioallied Sciences0976-48790975-74062025-06-0117Suppl 2S1371S137310.4103/jpbs.jpbs_1653_24Effect of Different Irrigating Solutions on the Microhardness of DentinSaron Ramesh NairMustafa Hussein AlattasLuluah Khalid AlhagasJohn Kodumbilayiparambil AntoNasarudheen CholayilAbdul Sathar MalayilBackground: The selection of irrigating solutions during root canal therapy is essential for maintaining dentin structure and ensuring effective disinfection. However, various irrigants can alter the microhardness of dentin, which might have an impact on the treated tooth’s mechanical characteristics and long-term viability. Materials and Methods: To produce uniform dentin discs from the central portion of the root, forty human premolars were sectioned. Based on the irrigating solution employed, the specimens were split into four groups (n = 10): Group 1 received 2.5% sodium hypochlorite (NaOCl), Group 2 received 17% ethylenediaminetetraacetic acid (EDTA), Group 3 received 2% chlorhexidine (CHX), and Group 4 received distilled water (control). Each specimen was submerged in its respective irrigant for five minutes. A Vickers microhardness tester was used to determine microhardness at 50 g force for 10 seconds both before and after irrigation. Results: Microhardness decreased significantly in Group 1 (NaOCl), with a mean drop of 18% (from 55 ± 2.3 VHN to 45 ± 1.9 VHN). The greatest reduction, 28%, was observed in Group 2 (EDTA) (from 53 ± 1.8 VHN to 38 ± 2.1 VHN). The microhardness of Group 3 (CHX) decreased by only 5% (from 54 ± 2.0 VHN to 51 ± 1.5 VHN). Microhardness showed no significant change in the control group (Group 4). Significant differences between the groups were found by statistical analysis (ANOVA, P < 0.05), with Group 2 exhibiting the greatest influence on dentin microhardness. Conclusion: Dentin microhardness was most significantly reduced by EDTA, followed by NaOCl, with CHX showing only a minor effect. These results imply that frequent or extended usage of EDTA and NaOCl may cause the dentin structure to deteriorate.https://journals.lww.com/10.4103/jpbs.jpbs_1653_24chlorhexidinedentin microhardnessedtairrigating solutionsroot canal treatmentsodium hypochloritevickers hardness |
| spellingShingle | Saron Ramesh Nair Mustafa Hussein Alattas Luluah Khalid Alhagas John Kodumbilayiparambil Anto Nasarudheen Cholayil Abdul Sathar Malayil Effect of Different Irrigating Solutions on the Microhardness of Dentin Journal of Pharmacy and Bioallied Sciences chlorhexidine dentin microhardness edta irrigating solutions root canal treatment sodium hypochlorite vickers hardness |
| title | Effect of Different Irrigating Solutions on the Microhardness of Dentin |
| title_full | Effect of Different Irrigating Solutions on the Microhardness of Dentin |
| title_fullStr | Effect of Different Irrigating Solutions on the Microhardness of Dentin |
| title_full_unstemmed | Effect of Different Irrigating Solutions on the Microhardness of Dentin |
| title_short | Effect of Different Irrigating Solutions on the Microhardness of Dentin |
| title_sort | effect of different irrigating solutions on the microhardness of dentin |
| topic | chlorhexidine dentin microhardness edta irrigating solutions root canal treatment sodium hypochlorite vickers hardness |
| url | https://journals.lww.com/10.4103/jpbs.jpbs_1653_24 |
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