Genetic diversity analysis and core germplasm construction of tea plants in Lu’an
Abstract Background Lu’an, as one of the two major tea-producing areas in Anhui Province, has a long history of tea planting and rich tea germplasm resources. However, the genetic diversity and population structure of local tea plants are still unclear. In order to better protect and utilize tea ger...
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BMC
2025-02-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-025-06216-3 |
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| author | Yan Wang Huanyun Peng Xiaoyan Tong Xiaoyuan Ding Cheng Song Teng Ma Haohao Wang Wang Wei Cunwu Chen Junyan Zhu Dong Liu |
| author_facet | Yan Wang Huanyun Peng Xiaoyan Tong Xiaoyuan Ding Cheng Song Teng Ma Haohao Wang Wang Wei Cunwu Chen Junyan Zhu Dong Liu |
| author_sort | Yan Wang |
| collection | DOAJ |
| description | Abstract Background Lu’an, as one of the two major tea-producing areas in Anhui Province, has a long history of tea planting and rich tea germplasm resources. However, the genetic diversity and population structure of local tea plants are still unclear. In order to better protect and utilize tea germplasm resources in Lu’an, 217 tea accessions from six geographical origins were used to assess genetic diversity of Lu’an tea plant germplasm through double digest restriction-site associated DNA sequencing (ddRAD-seq) technology. Results A total of 306,320 high quality single nucleotide polymorphism (SNP) markers were obtained. Population structure, phylogenetic relationships and principal component analysis (PCA) divided the entire population into three groups. The genetic diversity and population differentiation analysis showed that the mean observed heterozygosity (Ho) was 0.06 ∼ 0.17, average nucleotide diversity (Pi) was 0.13 ∼ 0.26, and pairwise fixation index (Fst) was 0.01 ∼ 0.15. In addition, a core tea germplasm set composed of 50 tea germplasm sets was established. Conclusion Our study demonstrated that the germplasm resources of the Lu’an tea plants exhibit significant genetic diversity. A core germplasm sets for the Lu’an tea plants has been established, which effectively represents the genetic diversity of the entire tea germplasm collection. This study provided the basis for genetic research, germplasm protection and breeding of tea plants in Lu’an. |
| format | Article |
| id | doaj-art-83d649c0833d48bdbb1c89c6827e3371 |
| institution | OA Journals |
| issn | 1471-2229 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj-art-83d649c0833d48bdbb1c89c6827e33712025-08-20T02:01:35ZengBMCBMC Plant Biology1471-22292025-02-0125111110.1186/s12870-025-06216-3Genetic diversity analysis and core germplasm construction of tea plants in Lu’anYan Wang0Huanyun Peng1Xiaoyan Tong2Xiaoyuan Ding3Cheng Song4Teng Ma5Haohao Wang6Wang Wei7Cunwu Chen8Junyan Zhu9Dong Liu10College of Biological and Pharmaceutical Engineering, West Anhui UniversityState Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural UniversityState Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural UniversityCollege of Biological and Pharmaceutical Engineering, West Anhui UniversityCollege of Biological and Pharmaceutical Engineering, West Anhui UniversityCollege of Biological and Pharmaceutical Engineering, West Anhui UniversityCollege of Biological and Pharmaceutical Engineering, West Anhui UniversityCollege of Biological and Pharmaceutical Engineering, West Anhui UniversityCollege of Biological and Pharmaceutical Engineering, West Anhui UniversityState Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural UniversityCollege of Biological and Pharmaceutical Engineering, West Anhui UniversityAbstract Background Lu’an, as one of the two major tea-producing areas in Anhui Province, has a long history of tea planting and rich tea germplasm resources. However, the genetic diversity and population structure of local tea plants are still unclear. In order to better protect and utilize tea germplasm resources in Lu’an, 217 tea accessions from six geographical origins were used to assess genetic diversity of Lu’an tea plant germplasm through double digest restriction-site associated DNA sequencing (ddRAD-seq) technology. Results A total of 306,320 high quality single nucleotide polymorphism (SNP) markers were obtained. Population structure, phylogenetic relationships and principal component analysis (PCA) divided the entire population into three groups. The genetic diversity and population differentiation analysis showed that the mean observed heterozygosity (Ho) was 0.06 ∼ 0.17, average nucleotide diversity (Pi) was 0.13 ∼ 0.26, and pairwise fixation index (Fst) was 0.01 ∼ 0.15. In addition, a core tea germplasm set composed of 50 tea germplasm sets was established. Conclusion Our study demonstrated that the germplasm resources of the Lu’an tea plants exhibit significant genetic diversity. A core germplasm sets for the Lu’an tea plants has been established, which effectively represents the genetic diversity of the entire tea germplasm collection. This study provided the basis for genetic research, germplasm protection and breeding of tea plants in Lu’an.https://doi.org/10.1186/s12870-025-06216-3Tea plantsddRAD-seqGenetic diversityPopulation structureCore collection |
| spellingShingle | Yan Wang Huanyun Peng Xiaoyan Tong Xiaoyuan Ding Cheng Song Teng Ma Haohao Wang Wang Wei Cunwu Chen Junyan Zhu Dong Liu Genetic diversity analysis and core germplasm construction of tea plants in Lu’an BMC Plant Biology Tea plants ddRAD-seq Genetic diversity Population structure Core collection |
| title | Genetic diversity analysis and core germplasm construction of tea plants in Lu’an |
| title_full | Genetic diversity analysis and core germplasm construction of tea plants in Lu’an |
| title_fullStr | Genetic diversity analysis and core germplasm construction of tea plants in Lu’an |
| title_full_unstemmed | Genetic diversity analysis and core germplasm construction of tea plants in Lu’an |
| title_short | Genetic diversity analysis and core germplasm construction of tea plants in Lu’an |
| title_sort | genetic diversity analysis and core germplasm construction of tea plants in lu an |
| topic | Tea plants ddRAD-seq Genetic diversity Population structure Core collection |
| url | https://doi.org/10.1186/s12870-025-06216-3 |
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