HPLC method with monolithic column for simultaneous determination of irbesartan and hydrochlorothiazide in tablets
A simple, sensitive and accurate HPLC method with high throughput has been developed and validated for the simultaneous determination of irbesartan (IRB) and hydrochlorothiazide (HCT) in combined pharmaceutical dosage forms. The proposed method employed, for the first time, a monolithic column in th...
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Sciendo
2014-06-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | http://www.degruyter.com/view/j/acph.2014.64.issue-2/acph-2014-0014/acph-2014-0014.xml?format=INT |
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| author | Alanazi Amer M. Abdelhameed Ali S. Khalil Nasr Y. Khan Azmat A. Darwish Ibrahim A. |
| author_facet | Alanazi Amer M. Abdelhameed Ali S. Khalil Nasr Y. Khan Azmat A. Darwish Ibrahim A. |
| author_sort | Alanazi Amer M. |
| collection | DOAJ |
| description | A simple, sensitive and accurate HPLC method with high throughput has been developed and validated for the simultaneous determination of irbesartan (IRB) and hydrochlorothiazide (HCT) in combined pharmaceutical dosage forms. The proposed method employed, for the first time, a monolithic column in the analysis. Optimal chromatographic separation of the analytes was achieved on Chromolith® Performance RP-18e column using a mobile phase consisting of phosphate buffer (pH 4)/acetonitrile (50:50, V/V) pumped isocratically at a flow rate of 1.0 mL min-1. The eluted analytes were monitored with a UV detector set at 270 nm. Under the optimum chromatographic conditions, linear relationship with a good correlation coefficient (R ≥ 0.9997) was found between the peak area and the corresponding concentrations of both IRB and HCT in the ranges of 10-200 and 1-20 ng mL-1. The limits of detection were 2.34 and 0.03 ng mL-1 for IRB and HCT, respectively. The intra- and inter-assay precisions were satisfactory as the RSD values did not exceed 3 %. The accuracy of the proposed method was > 97 %. The proposed method had high throughput as the analysis involved a simple procedure and a very short run- -time of < 3 min. The results demonstrated that the method is applicable in the quality control of combined pharmaceutical tablets containing IRB and HCT |
| format | Article |
| id | doaj-art-83d0371cb9314b97bbfb392995cf552b |
| institution | Kabale University |
| issn | 1330-0075 1846-9558 |
| language | English |
| publishDate | 2014-06-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-83d0371cb9314b97bbfb392995cf552b2025-02-02T00:04:16ZengSciendoActa Pharmaceutica1330-00751846-95582014-06-0164218719810.2478/acph-2014-0014acph-2014-0014HPLC method with monolithic column for simultaneous determination of irbesartan and hydrochlorothiazide in tabletsAlanazi Amer M.0Abdelhameed Ali S.1Khalil Nasr Y.2Khan Azmat A.3Darwish Ibrahim A.4Department of Pharmaceutical Chemistry College of Pharmacy, King Saud University P.O. Box 2457 Riyadh, 11451 Kingdom of Saudi ArabiaDepartment of Pharmaceutical Chemistry College of Pharmacy, King Saud University P.O. Box 2457 Riyadh, 11451 Kingdom of Saudi ArabiaDepartment of Pharmaceutical Chemistry College of Pharmacy, King Saud University P.O. Box 2457 Riyadh, 11451 Kingdom of Saudi ArabiaDepartment of Pharmaceutical Chemistry College of Pharmacy, King Saud University P.O. Box 2457 Riyadh, 11451 Kingdom of Saudi ArabiaDepartment of Pharmaceutical Chemistry College of Pharmacy, King Saud University P.O. Box 2457 Riyadh, 11451 Kingdom of Saudi ArabiaA simple, sensitive and accurate HPLC method with high throughput has been developed and validated for the simultaneous determination of irbesartan (IRB) and hydrochlorothiazide (HCT) in combined pharmaceutical dosage forms. The proposed method employed, for the first time, a monolithic column in the analysis. Optimal chromatographic separation of the analytes was achieved on Chromolith® Performance RP-18e column using a mobile phase consisting of phosphate buffer (pH 4)/acetonitrile (50:50, V/V) pumped isocratically at a flow rate of 1.0 mL min-1. The eluted analytes were monitored with a UV detector set at 270 nm. Under the optimum chromatographic conditions, linear relationship with a good correlation coefficient (R ≥ 0.9997) was found between the peak area and the corresponding concentrations of both IRB and HCT in the ranges of 10-200 and 1-20 ng mL-1. The limits of detection were 2.34 and 0.03 ng mL-1 for IRB and HCT, respectively. The intra- and inter-assay precisions were satisfactory as the RSD values did not exceed 3 %. The accuracy of the proposed method was > 97 %. The proposed method had high throughput as the analysis involved a simple procedure and a very short run- -time of < 3 min. The results demonstrated that the method is applicable in the quality control of combined pharmaceutical tablets containing IRB and HCThttp://www.degruyter.com/view/j/acph.2014.64.issue-2/acph-2014-0014/acph-2014-0014.xml?format=INTirbesartanhydrochlorothiazideHPLC-UVmonolithic column |
| spellingShingle | Alanazi Amer M. Abdelhameed Ali S. Khalil Nasr Y. Khan Azmat A. Darwish Ibrahim A. HPLC method with monolithic column for simultaneous determination of irbesartan and hydrochlorothiazide in tablets Acta Pharmaceutica irbesartan hydrochlorothiazide HPLC-UV monolithic column |
| title | HPLC method with monolithic column for simultaneous determination of irbesartan and hydrochlorothiazide in tablets |
| title_full | HPLC method with monolithic column for simultaneous determination of irbesartan and hydrochlorothiazide in tablets |
| title_fullStr | HPLC method with monolithic column for simultaneous determination of irbesartan and hydrochlorothiazide in tablets |
| title_full_unstemmed | HPLC method with monolithic column for simultaneous determination of irbesartan and hydrochlorothiazide in tablets |
| title_short | HPLC method with monolithic column for simultaneous determination of irbesartan and hydrochlorothiazide in tablets |
| title_sort | hplc method with monolithic column for simultaneous determination of irbesartan and hydrochlorothiazide in tablets |
| topic | irbesartan hydrochlorothiazide HPLC-UV monolithic column |
| url | http://www.degruyter.com/view/j/acph.2014.64.issue-2/acph-2014-0014/acph-2014-0014.xml?format=INT |
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