iTRAQ-Based Protein Profiling in CUMS Rats Provides Insights into Hippocampal Ribosome Lesion and Ras Protein Changes Underlying Synaptic Plasticity in Depression
Hippocampal atrophy is one of the key changes in the brain implicated in the biology of depression. However, the precise molecular mechanism remains poorly understood due to a lack of biomarkers. In this research, we used behavioral experiments to evaluate anxiety and anhedonia levels in depressed r...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2019/7492306 |
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| author | Jialing Zhang Zhinan Zhang Jiping Zhang Zheng Zhong Zengyu Yao Shanshan Qu Yong Huang |
| author_facet | Jialing Zhang Zhinan Zhang Jiping Zhang Zheng Zhong Zengyu Yao Shanshan Qu Yong Huang |
| author_sort | Jialing Zhang |
| collection | DOAJ |
| description | Hippocampal atrophy is one of the key changes in the brain implicated in the biology of depression. However, the precise molecular mechanism remains poorly understood due to a lack of biomarkers. In this research, we used behavioral experiments to evaluate anxiety and anhedonia levels in depressed rats using chronic unpredictable mild stress (CUMS) modeling. We also used isobaric tag for relative and absolute quantitation (iTRAQ) to identify the differentially expressed hippocampal proteins between depressed and normal rats. Bioinformatics analyses were also performed for a better understanding. The results showed that CUMS rats had higher anxiety and anhedonia levels than control rats, along with hippocampal lesions. Through iTRAQ and bioinformatics analyses, we found that ribosome proteins were significantly downregulated and Ras proteins exhibited a mixed change in the hippocampus of depressed rats. These findings suggest that the expression of hippocampal ribosome lesions and Ras proteins is significantly different in depressed rats than in control rats, providing new insights into the neurobiology of depression. |
| format | Article |
| id | doaj-art-83c7afd744f74732913f5177ebc96921 |
| institution | OA Journals |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-83c7afd744f74732913f5177ebc969212025-08-20T02:09:07ZengWileyNeural Plasticity2090-59041687-54432019-01-01201910.1155/2019/74923067492306iTRAQ-Based Protein Profiling in CUMS Rats Provides Insights into Hippocampal Ribosome Lesion and Ras Protein Changes Underlying Synaptic Plasticity in DepressionJialing Zhang0Zhinan Zhang1Jiping Zhang2Zheng Zhong3Zengyu Yao4Shanshan Qu5Yong Huang6School of Chinese Medicine, The University of Hong Kong, 999077, Hong KongSchool of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong Province, 510515, ChinaSchool of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong Province, 510515, ChinaNanfang Hospital, Southern Medical University, Guangzhou, Guangdong Province, 510515, ChinaSchool of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong Province, 510515, ChinaSchool of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong Province, 510515, ChinaSchool of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong Province, 510515, ChinaHippocampal atrophy is one of the key changes in the brain implicated in the biology of depression. However, the precise molecular mechanism remains poorly understood due to a lack of biomarkers. In this research, we used behavioral experiments to evaluate anxiety and anhedonia levels in depressed rats using chronic unpredictable mild stress (CUMS) modeling. We also used isobaric tag for relative and absolute quantitation (iTRAQ) to identify the differentially expressed hippocampal proteins between depressed and normal rats. Bioinformatics analyses were also performed for a better understanding. The results showed that CUMS rats had higher anxiety and anhedonia levels than control rats, along with hippocampal lesions. Through iTRAQ and bioinformatics analyses, we found that ribosome proteins were significantly downregulated and Ras proteins exhibited a mixed change in the hippocampus of depressed rats. These findings suggest that the expression of hippocampal ribosome lesions and Ras proteins is significantly different in depressed rats than in control rats, providing new insights into the neurobiology of depression.http://dx.doi.org/10.1155/2019/7492306 |
| spellingShingle | Jialing Zhang Zhinan Zhang Jiping Zhang Zheng Zhong Zengyu Yao Shanshan Qu Yong Huang iTRAQ-Based Protein Profiling in CUMS Rats Provides Insights into Hippocampal Ribosome Lesion and Ras Protein Changes Underlying Synaptic Plasticity in Depression Neural Plasticity |
| title | iTRAQ-Based Protein Profiling in CUMS Rats Provides Insights into Hippocampal Ribosome Lesion and Ras Protein Changes Underlying Synaptic Plasticity in Depression |
| title_full | iTRAQ-Based Protein Profiling in CUMS Rats Provides Insights into Hippocampal Ribosome Lesion and Ras Protein Changes Underlying Synaptic Plasticity in Depression |
| title_fullStr | iTRAQ-Based Protein Profiling in CUMS Rats Provides Insights into Hippocampal Ribosome Lesion and Ras Protein Changes Underlying Synaptic Plasticity in Depression |
| title_full_unstemmed | iTRAQ-Based Protein Profiling in CUMS Rats Provides Insights into Hippocampal Ribosome Lesion and Ras Protein Changes Underlying Synaptic Plasticity in Depression |
| title_short | iTRAQ-Based Protein Profiling in CUMS Rats Provides Insights into Hippocampal Ribosome Lesion and Ras Protein Changes Underlying Synaptic Plasticity in Depression |
| title_sort | itraq based protein profiling in cums rats provides insights into hippocampal ribosome lesion and ras protein changes underlying synaptic plasticity in depression |
| url | http://dx.doi.org/10.1155/2019/7492306 |
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