Olive oil protects against cardiac hypertrophy in D-galactose induced aging rats
Abstract Background Aged heart is defined via structural and mitochondrial dysfunction of the heart. However, there is still no potent compound to improve cardiac function abnormalities in aged individuals. Olive oil (OLO), as an oil with monounsaturated fatty acids, has diverse protective effects o...
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BMC
2024-11-01
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| Series: | BMC Cardiovascular Disorders |
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| Online Access: | https://doi.org/10.1186/s12872-024-04278-z |
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| author | Siamak Shahidi Khadijeh Ramezani-Aliakbari Abdolrahman Sarihi Ali Heshmati Elham Shiri Shiva Nosrati Sayedpayam Hashemi Mitra Bahrami Fatemeh Ramezani-Aliakbari |
| author_facet | Siamak Shahidi Khadijeh Ramezani-Aliakbari Abdolrahman Sarihi Ali Heshmati Elham Shiri Shiva Nosrati Sayedpayam Hashemi Mitra Bahrami Fatemeh Ramezani-Aliakbari |
| author_sort | Siamak Shahidi |
| collection | DOAJ |
| description | Abstract Background Aged heart is defined via structural and mitochondrial dysfunction of the heart. However, there is still no potent compound to improve cardiac function abnormalities in aged individuals. Olive oil (OLO), as an oil with monounsaturated fatty acids, has diverse protective effects on the cardiovascular system, including anti-inflammatory, anti-diabetic, and mitigating effects on blood pressure. In the present study, we evaluated the protective effects of OLO against aging-related cardiac dysfunction. Methods Male Wistar rats were randomly divided into three groups: Control, D-galactose-induced aging rats (D-GAL group), and aging rats treated with OLO (D-GAL + OLO group). Aging in rats was induced by intraperitoneal injection of D-GAL at 150 mg/kg dose for eight weeks and the D-GAL + OLO group was treated with oral OLO by gavage for eight weeks. The heart tissues were harvested to assay the oxidative stress, molecular parameters, and histological analysis. Results The D-GAL given rats indicated increased cardiomyocyte diameter as cardiac hypertrophy marker (21 ± 0.8, p < 0.001), an increased Malondialdehyde (MDA) level (27 ± 3, p < 0.001), a reduced Superoxide dismutase (SOD) (p < 0.001, 18.12 ± 1.3), and reduction in gene expression of Sirtuin 1 (SIRT1) (p < 0.05, 0.37 ± 0.06), Peroxisome proliferator-activated receptor-gamma coactivator (PGC)-1α (p < 0.001, 0.027 ± 0.04), and Transcription Factor A, Mitochondrial (TFAM) (p < 0.001, 0.023 ± 0.01), Bcl2 (p < 0.001, 0.04 ± 0.004) and an increase in gene expression of Bax (p < 0.001, 23.5 ± 5.4) in comparison with the control animals. Treatment with OLO improved cardiac hypertrophy (14 ± 0.4, p < 0.001), MDA (22 ± 2.5, p < 0.01), SOD (p < 0.001, 34.9 ± 2), SIRT1 (p < 0.05, 1.37 ± 0.46), PGC-1α (p < 0.001, 1.11 ± 0.1), TFAM (p < 0.01, 0.23 ± 0.02), Bcl2 (p < 0.05, 0.35 ± 0.05) and Bax genes (p < 0.01, 0.1 ± 0.03). Conclusions Overall, OLO protects the heart against D-GAL-induced aging via increasing antioxidant effects, and enhancing cardiac expression of SIRT1, PGC-1α, TFAM, Bcl2 and Bax genes. Graphical Abstract |
| format | Article |
| id | doaj-art-83ba7f47de344ba09b0a09cfe901ce3d |
| institution | OA Journals |
| issn | 1471-2261 |
| language | English |
| publishDate | 2024-11-01 |
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| series | BMC Cardiovascular Disorders |
| spelling | doaj-art-83ba7f47de344ba09b0a09cfe901ce3d2025-08-20T02:13:27ZengBMCBMC Cardiovascular Disorders1471-22612024-11-012411910.1186/s12872-024-04278-zOlive oil protects against cardiac hypertrophy in D-galactose induced aging ratsSiamak Shahidi0Khadijeh Ramezani-Aliakbari1Abdolrahman Sarihi2Ali Heshmati3Elham Shiri4Shiva Nosrati5Sayedpayam Hashemi6Mitra Bahrami7Fatemeh Ramezani-Aliakbari8Department of Physiology, School of medicine, Hamadan University of Medical SciencesDepartment of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina UniversityNeurophysiology Research Center, Hamadan University of Medical SciencesDepartment of Nutrition and Food Safety, School of Medicine, Nutrition Health Research Center, Hamadan University of Medical SciencesNeurophysiology Research Center, Hamadan University of Medical SciencesDepartment of Neuroscience, Hamadan University of Medical SciencesMedical School, Hamadan University of Medical SciencesDepartment of Islamic Studies, School of Medicine, Kermanshah University of Medical SciencesDepartment of Physiology, School of medicine, Hamadan University of Medical SciencesAbstract Background Aged heart is defined via structural and mitochondrial dysfunction of the heart. However, there is still no potent compound to improve cardiac function abnormalities in aged individuals. Olive oil (OLO), as an oil with monounsaturated fatty acids, has diverse protective effects on the cardiovascular system, including anti-inflammatory, anti-diabetic, and mitigating effects on blood pressure. In the present study, we evaluated the protective effects of OLO against aging-related cardiac dysfunction. Methods Male Wistar rats were randomly divided into three groups: Control, D-galactose-induced aging rats (D-GAL group), and aging rats treated with OLO (D-GAL + OLO group). Aging in rats was induced by intraperitoneal injection of D-GAL at 150 mg/kg dose for eight weeks and the D-GAL + OLO group was treated with oral OLO by gavage for eight weeks. The heart tissues were harvested to assay the oxidative stress, molecular parameters, and histological analysis. Results The D-GAL given rats indicated increased cardiomyocyte diameter as cardiac hypertrophy marker (21 ± 0.8, p < 0.001), an increased Malondialdehyde (MDA) level (27 ± 3, p < 0.001), a reduced Superoxide dismutase (SOD) (p < 0.001, 18.12 ± 1.3), and reduction in gene expression of Sirtuin 1 (SIRT1) (p < 0.05, 0.37 ± 0.06), Peroxisome proliferator-activated receptor-gamma coactivator (PGC)-1α (p < 0.001, 0.027 ± 0.04), and Transcription Factor A, Mitochondrial (TFAM) (p < 0.001, 0.023 ± 0.01), Bcl2 (p < 0.001, 0.04 ± 0.004) and an increase in gene expression of Bax (p < 0.001, 23.5 ± 5.4) in comparison with the control animals. Treatment with OLO improved cardiac hypertrophy (14 ± 0.4, p < 0.001), MDA (22 ± 2.5, p < 0.01), SOD (p < 0.001, 34.9 ± 2), SIRT1 (p < 0.05, 1.37 ± 0.46), PGC-1α (p < 0.001, 1.11 ± 0.1), TFAM (p < 0.01, 0.23 ± 0.02), Bcl2 (p < 0.05, 0.35 ± 0.05) and Bax genes (p < 0.01, 0.1 ± 0.03). Conclusions Overall, OLO protects the heart against D-GAL-induced aging via increasing antioxidant effects, and enhancing cardiac expression of SIRT1, PGC-1α, TFAM, Bcl2 and Bax genes. Graphical Abstracthttps://doi.org/10.1186/s12872-024-04278-zAgingAntioxidantsHeartMitochondriaOlive Oil |
| spellingShingle | Siamak Shahidi Khadijeh Ramezani-Aliakbari Abdolrahman Sarihi Ali Heshmati Elham Shiri Shiva Nosrati Sayedpayam Hashemi Mitra Bahrami Fatemeh Ramezani-Aliakbari Olive oil protects against cardiac hypertrophy in D-galactose induced aging rats BMC Cardiovascular Disorders Aging Antioxidants Heart Mitochondria Olive Oil |
| title | Olive oil protects against cardiac hypertrophy in D-galactose induced aging rats |
| title_full | Olive oil protects against cardiac hypertrophy in D-galactose induced aging rats |
| title_fullStr | Olive oil protects against cardiac hypertrophy in D-galactose induced aging rats |
| title_full_unstemmed | Olive oil protects against cardiac hypertrophy in D-galactose induced aging rats |
| title_short | Olive oil protects against cardiac hypertrophy in D-galactose induced aging rats |
| title_sort | olive oil protects against cardiac hypertrophy in d galactose induced aging rats |
| topic | Aging Antioxidants Heart Mitochondria Olive Oil |
| url | https://doi.org/10.1186/s12872-024-04278-z |
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