Vaccination of poultry against highly pathogenic avian influenza – Part 2. Surveillance and mitigation measures

Abstract Selecting appropriate diagnostic methods that take account of the type of vaccine used is important when implementing a vaccination programme against highly pathogenic avian influenza (HPAI). If vaccination is effective, a decreased viral load is expected in the samples used for diagnosis,...

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Main Authors: EFSA Panel on Animal Health and Animal Welfare (AHAW), European Union Reference Laboratory for Avian Influenza, Søren Saxmose Nielsen, Julio Alvarez, Dominique Joseph Bicout, Paolo Calistri, Elisabetta Canali, Julian Ashley Drewe, Bruno Garin‐Bastuji, Christian Gortázar, Mette S. Herskin, Virginie Michel, Miguel Ángel Miranda Chueca, Barbara Padalino, Helen Clare Roberts, Hans Spoolder, Karl Stahl, Antonio Velarde, Arvo Viltrop, Christoph Winckler, Alessio Bortolami, Claire Guinat, Timm Harder, Arjan Stegeman, Calogero Terregino, Barbara Lanfranchi, Ludovica Preite, Inma Aznar, Alessandro Broglia, Francesca Baldinelli, Jose Luis Gonzales Rojas
Format: Article
Language:English
Published: Wiley 2024-04-01
Series:EFSA Journal
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Online Access:https://doi.org/10.2903/j.efsa.2024.8755
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author EFSA Panel on Animal Health and Animal Welfare (AHAW)
European Union Reference Laboratory for Avian Influenza
Søren Saxmose Nielsen
Julio Alvarez
Dominique Joseph Bicout
Paolo Calistri
Elisabetta Canali
Julian Ashley Drewe
Bruno Garin‐Bastuji
Christian Gortázar
Mette S. Herskin
Virginie Michel
Miguel Ángel Miranda Chueca
Barbara Padalino
Helen Clare Roberts
Hans Spoolder
Karl Stahl
Antonio Velarde
Arvo Viltrop
Christoph Winckler
Alessio Bortolami
Claire Guinat
Timm Harder
Arjan Stegeman
Calogero Terregino
Barbara Lanfranchi
Ludovica Preite
Inma Aznar
Alessandro Broglia
Francesca Baldinelli
Jose Luis Gonzales Rojas
author_facet EFSA Panel on Animal Health and Animal Welfare (AHAW)
European Union Reference Laboratory for Avian Influenza
Søren Saxmose Nielsen
Julio Alvarez
Dominique Joseph Bicout
Paolo Calistri
Elisabetta Canali
Julian Ashley Drewe
Bruno Garin‐Bastuji
Christian Gortázar
Mette S. Herskin
Virginie Michel
Miguel Ángel Miranda Chueca
Barbara Padalino
Helen Clare Roberts
Hans Spoolder
Karl Stahl
Antonio Velarde
Arvo Viltrop
Christoph Winckler
Alessio Bortolami
Claire Guinat
Timm Harder
Arjan Stegeman
Calogero Terregino
Barbara Lanfranchi
Ludovica Preite
Inma Aznar
Alessandro Broglia
Francesca Baldinelli
Jose Luis Gonzales Rojas
author_sort EFSA Panel on Animal Health and Animal Welfare (AHAW)
collection DOAJ
description Abstract Selecting appropriate diagnostic methods that take account of the type of vaccine used is important when implementing a vaccination programme against highly pathogenic avian influenza (HPAI). If vaccination is effective, a decreased viral load is expected in the samples used for diagnosis, making molecular methods with high sensitivity the best choice. Although serological methods can be reasonably sensitive, they may produce results that are difficult to interpret. In addition to routine molecular monitoring, it is recommended to conduct viral isolation, genetic sequencing and phenotypic characterisation of any HPAI virus detected in vaccinated flocks to detect escape mutants early. Following emergency vaccination, various surveillance options based on virological testing of dead birds (‘bucket sampling’) at defined intervals were assessed to be effective for early detection of HPAIV and prove disease freedom in vaccinated populations. For ducks, virological or serological testing of live birds was assessed as an effective strategy. This surveillance could be also applied in the peri‐vaccination zone on vaccinated establishments, while maintaining passive surveillance in unvaccinated chicken layers and turkeys, and weekly bucket sampling in unvaccinated ducks. To demonstrate disease freedom with > 99% confidence and to detect HPAI virus sufficiently early following preventive vaccination, monthly virological testing of all dead birds up to 15 per flock, coupled with passive surveillance in both vaccinated and unvaccinated flocks, is recommended. Reducing the sampling intervals increases the sensitivity of early detection up to 100%. To enable the safe movement of vaccinated poultry during emergency vaccination, laboratory examinations in the 72 h prior to the movement can be considered as a risk mitigation measure, in addition to clinical inspection; sampling results from existing surveillance activities carried out in these 72 h could be used. In this Opinion, several schemes are recommended to enable the safe movement of vaccinated poultry following preventive vaccination.
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spelling doaj-art-83ba55960b1f42e791f91a36ecd715502025-08-20T03:28:58ZengWileyEFSA Journal1831-47322024-04-01224n/an/a10.2903/j.efsa.2024.8755Vaccination of poultry against highly pathogenic avian influenza – Part 2. Surveillance and mitigation measuresEFSA Panel on Animal Health and Animal Welfare (AHAW)European Union Reference Laboratory for Avian InfluenzaSøren Saxmose NielsenJulio AlvarezDominique Joseph BicoutPaolo CalistriElisabetta CanaliJulian Ashley DreweBruno Garin‐BastujiChristian GortázarMette S. HerskinVirginie MichelMiguel Ángel Miranda ChuecaBarbara PadalinoHelen Clare RobertsHans SpoolderKarl StahlAntonio VelardeArvo ViltropChristoph WincklerAlessio BortolamiClaire GuinatTimm HarderArjan StegemanCalogero TerreginoBarbara LanfranchiLudovica PreiteInma AznarAlessandro BrogliaFrancesca BaldinelliJose Luis Gonzales RojasAbstract Selecting appropriate diagnostic methods that take account of the type of vaccine used is important when implementing a vaccination programme against highly pathogenic avian influenza (HPAI). If vaccination is effective, a decreased viral load is expected in the samples used for diagnosis, making molecular methods with high sensitivity the best choice. Although serological methods can be reasonably sensitive, they may produce results that are difficult to interpret. In addition to routine molecular monitoring, it is recommended to conduct viral isolation, genetic sequencing and phenotypic characterisation of any HPAI virus detected in vaccinated flocks to detect escape mutants early. Following emergency vaccination, various surveillance options based on virological testing of dead birds (‘bucket sampling’) at defined intervals were assessed to be effective for early detection of HPAIV and prove disease freedom in vaccinated populations. For ducks, virological or serological testing of live birds was assessed as an effective strategy. This surveillance could be also applied in the peri‐vaccination zone on vaccinated establishments, while maintaining passive surveillance in unvaccinated chicken layers and turkeys, and weekly bucket sampling in unvaccinated ducks. To demonstrate disease freedom with > 99% confidence and to detect HPAI virus sufficiently early following preventive vaccination, monthly virological testing of all dead birds up to 15 per flock, coupled with passive surveillance in both vaccinated and unvaccinated flocks, is recommended. Reducing the sampling intervals increases the sensitivity of early detection up to 100%. To enable the safe movement of vaccinated poultry during emergency vaccination, laboratory examinations in the 72 h prior to the movement can be considered as a risk mitigation measure, in addition to clinical inspection; sampling results from existing surveillance activities carried out in these 72 h could be used. In this Opinion, several schemes are recommended to enable the safe movement of vaccinated poultry following preventive vaccination.https://doi.org/10.2903/j.efsa.2024.8755avian influenza transmissionhighly pathogenic avian influenza (HPAI)poultrysurveillancesurveillance strategies
spellingShingle EFSA Panel on Animal Health and Animal Welfare (AHAW)
European Union Reference Laboratory for Avian Influenza
Søren Saxmose Nielsen
Julio Alvarez
Dominique Joseph Bicout
Paolo Calistri
Elisabetta Canali
Julian Ashley Drewe
Bruno Garin‐Bastuji
Christian Gortázar
Mette S. Herskin
Virginie Michel
Miguel Ángel Miranda Chueca
Barbara Padalino
Helen Clare Roberts
Hans Spoolder
Karl Stahl
Antonio Velarde
Arvo Viltrop
Christoph Winckler
Alessio Bortolami
Claire Guinat
Timm Harder
Arjan Stegeman
Calogero Terregino
Barbara Lanfranchi
Ludovica Preite
Inma Aznar
Alessandro Broglia
Francesca Baldinelli
Jose Luis Gonzales Rojas
Vaccination of poultry against highly pathogenic avian influenza – Part 2. Surveillance and mitigation measures
EFSA Journal
avian influenza transmission
highly pathogenic avian influenza (HPAI)
poultry
surveillance
surveillance strategies
title Vaccination of poultry against highly pathogenic avian influenza – Part 2. Surveillance and mitigation measures
title_full Vaccination of poultry against highly pathogenic avian influenza – Part 2. Surveillance and mitigation measures
title_fullStr Vaccination of poultry against highly pathogenic avian influenza – Part 2. Surveillance and mitigation measures
title_full_unstemmed Vaccination of poultry against highly pathogenic avian influenza – Part 2. Surveillance and mitigation measures
title_short Vaccination of poultry against highly pathogenic avian influenza – Part 2. Surveillance and mitigation measures
title_sort vaccination of poultry against highly pathogenic avian influenza part 2 surveillance and mitigation measures
topic avian influenza transmission
highly pathogenic avian influenza (HPAI)
poultry
surveillance
surveillance strategies
url https://doi.org/10.2903/j.efsa.2024.8755
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