Analysis of the major carbon and nitrogen metabolic components in rice internodes based on near-infrared spectroscopy
The application of near-infrared spectroscopy has gained significant traction for crop sample component detection and rapid screening of breeding materials in recent years, while limited studies have been conducted on the high-throughput detection of the major carbon and nitrogen metabolic component...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2024-06-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2024.01.051 |
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| Summary: | The application of near-infrared spectroscopy has gained significant traction for crop sample component detection and rapid screening of breeding materials in recent years, while limited studies have been conducted on the high-throughput detection of the major carbon and nitrogen metabolic components in rice source-sink organs, particularly in rice internode samples. In this study, 576 representative internode samples obtained from the experiment were used as materials. On the basis of the chemical measured values of the samples, the near-infrared spectral information of the samples was collected, and the models of the major carbon and nitrogen metabolic components of the rice internodes, such as sucrose, starch and total nitrogen, were constructed via partial least square (PLS). The model validation results showed that the coefficients of determination of the sucrose, starch and total nitrogen contents of the validation sets in the PLS model of the rice internodes were 0.988, 0.977 and 0.957, respectively. The relative percent deviations were all greater than three, and the performance indexes were 85.20, 85.80 and 86.50, respectively, indicating that the predicted results of the established models were accurate and reliable. In addition, the PLS models of starch and nitrogen contents and C/N ratio of rice leaves and sheaths also exhibited high accuracy. In this study, the near-infrared spectroscopy model of the major carbon and nitrogen metabolic components of rice internodes was constructed, which provided technical support for the study of rice cultivation physiology and high-yield cultivation theory. |
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| ISSN: | 1008-9209 2097-5155 |